Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,437,182	A→G	I92T (ATC→ACC)	secY ←	preprotein translocase subunit SecY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,437,182	0	A	G	100.0%	175.6 / NA	49	I92T (ATC→ACC)	secY	preprotein translocase subunit SecY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/25);  total (24/25)

GGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGT  >  NZ_CP009273/3437046‑3437313                                                                                                                                         |                                                                                                                                    ggTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTa                                                                                                                                   <  1:126793/139‑1 (MQ=255)      cAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGAt                                                                                                                              <  1:801958/139‑1 (MQ=255)          aCCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGAc                                                                                                                          >  1:642702/1‑139 (MQ=255)           ccGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACg                                                                                                                         <  1:79147/139‑1 (MQ=255)             gATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGcc                                                                                                                       >  1:446449/1‑139 (MQ=255)             gATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGcc                                                                                                                       >  2:40766/1‑139 (MQ=255)             gATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGcc                                                                                                                       >  1:810388/1‑139 (MQ=255)             gATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGcc                                                                                                                       >  2:631986/1‑139 (MQ=255)                   cTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAata                                                                                                                 >  2:481786/1‑139 (MQ=255)                       aaTATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACg                                                                                                             <  1:38384/139‑1 (MQ=255)                            tGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCAt                                                                                                         >  1:149756/1‑138 (MQ=255)                            tGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCAt                                                                                                         >  1:668786/1‑138 (MQ=255)                             gCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATg                                                                                                        <  1:234991/138‑1 (MQ=255)                             gCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATg                                                                                                        <  1:741647/138‑1 (MQ=255)                                 gCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATccc                                                                                                   >  2:747647/1‑139 (MQ=255)                                  cACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATccc                                                                                                   >  1:833822/1‑138 (MQ=255)                                       gagTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGc                                                                                             <  2:471473/139‑1 (MQ=255)                                        agTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGc                                                                                             >  2:179366/1‑138 (MQ=255)                                        agTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCa                                                                                            <  1:40766/139‑1 (MQ=255)                                             cGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGa                                                                                        >  1:622204/1‑138 (MQ=255)                                               tAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGCACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGACCAACGa                                                                                        >  2:793116/1‑136 (MQ=255)                                               tAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGa                                                                                        >  1:542292/1‑136 (MQ=255)                                                 gCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGaa                                                                                   <  2:122486/139‑1 (MQ=255)                                                    ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                                <  1:530987/139‑1 (MQ=255)                                                    ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                                >  2:440942/1‑139 (MQ=255)                                                    ggTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCa                                                                                >  1:7937/1‑139 (MQ=255)                                                              cTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGagag                                                                      <  2:7937/139‑1 (MQ=255)                                                                  tCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcac                                                                   >  1:207912/1‑138 (MQ=255)                                                                      acgacgACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcac                                                                   <  1:735628/134‑1 (MQ=255)                                                                      acgacgACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcac                                                                   <  2:149756/134‑1 (MQ=255)                                                                       cgacgaCCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGcaccac                                                                >  1:478596/1‑136 (MQ=255)                                                                          cgacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAgaga                                                          <  2:207912/139‑1 (MQ=255)                                                                           gacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCg                                                                                                                      <  1:204963/78‑1 (MQ=255)                                                                           gacCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCg                                                                                                                      >  2:204963/1‑78 (MQ=255)                                                                            acCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGaa                                                         >  2:678759/1‑138 (MQ=255)                                                                                  cTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTa                                                  <  1:440942/139‑1 (MQ=255)                                                                                     cccTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa                                                <  2:446449/138‑1 (MQ=255)                                                                                             tcttAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACAtctc                                          <  1:92716/136‑1 (MQ=255)                                                                                             tcttAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAat                                       >  2:560615/1‑139 (MQ=255)                                                                                                       tGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCt                             >  1:540314/1‑139 (MQ=255)                                                                                                       tGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCt                             >  2:271766/1‑139 (MQ=255)                                                                                                         ccAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCt                             <  2:21538/137‑1 (MQ=255)                                                                                                         ccAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCt                             <  1:560615/137‑1 (MQ=255)                                                                                                         ccAACGTTGGGTGAACCACCGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCt                             <  1:678759/137‑1 (MQ=255)                                                                                                                         caccGTCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGt           <  1:457062/139‑1 (MQ=255)                                                                                                                              tCAGCAGCTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGc      <  1:10991/139‑1 (MQ=255)                                                                                                                                  cagcTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGCTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGt  <  1:747647/139‑1 (MQ=255)                                                                                                                                     cTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa                                                <  2:121149/90‑1 (MQ=255)                                                                                                                                     cTGGGTAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTaaa                                                >  1:121149/1‑90 (MQ=255)                                                                                                                                         |                                                                                                                                    GGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGGTGTACTGGCTGATCTTACGACGACCAGACTCCCCTTCTTTCTTAATTTCTGCCAACGTTGGGTGAACCACCGTCAGCAGCTGGATAATGATCGACGCCGAAATATACGGCATGATCCCCAGAGCAAAGATAGAAGCACGGCTGAGAGCACCACCAGAGAACATGTTAAACATCTCAATGATGGTGCCTCGCTGTTGCTCAAGCAGTTTGGCAAGT  >  NZ_CP009273/3437046‑3437313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑