Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,446,256	A→C	G10G (GGT→GGG)	rplC ←	50S ribosomal protein L3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,446,256	0	A	C	100.0%	178.8 / NA	50	G10G (GGT→GGG)	rplC	50S ribosomal protein L3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/25);  total (25/25)

GGTGGTCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATACCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGTTTTCT  >  NZ_CP009273/3446130‑3446388                                                                                                                               |                                                                                                                                     ggtggtCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTa                                                                                                                          <  1:110733/139‑1 (MQ=255) ggtggtCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTa                                                                                                                          <  2:151187/139‑1 (MQ=255)             aaTAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACtttttt                                                                                                                           >  2:778095/1‑126 (MQ=255)             aaTAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACtttttt                                                                                                                           <  1:778095/126‑1 (MQ=255)                  cACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCAt                                                                                                         >  2:619578/1‑139 (MQ=255)                  cACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCAt                                                                                                         >  1:411143/1‑139 (MQ=255)                       tAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGttt                                                                                                    >  2:196216/1‑139 (MQ=255)                       tAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGttt                                                                                                    >  2:170211/1‑139 (MQ=255)                       tAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGttt                                                                                                    >  2:484109/1‑139 (MQ=255)                         gCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   <  2:384596/138‑1 (MQ=255)                         gCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   <  1:706234/138‑1 (MQ=255)                             tCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACct                                                                                              <  1:170211/139‑1 (MQ=255)                               gTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACctct                                                                                            >  1:290939/1‑139 (MQ=255)                                       ggTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCt                                                                                    >  1:218265/1‑139 (MQ=255)                                        gTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATccc                                                                                                                                    >  1:144379/1‑90 (MQ=255)                                        gTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATccc                                                                                                                                    <  2:144379/90‑1 (MQ=255)                                        gTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCt                                                                                    >  1:293496/1‑138 (MQ=255)                                         tCTTTAACCTGAGTAACGCTGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCa                                                                                  <  2:125473/139‑1 (MQ=255)                                               aCCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAc                                                                             <  2:844026/138‑1 (MQ=255)                                                  tGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   >  2:246868/1‑113 (MQ=255)                                                  tGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTc                                                                                                   <  1:246868/113‑1 (MQ=255)                                                        aCGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAAc                                                                    <  1:619578/138‑1 (MQ=255)                                                        aCGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAAc                                                                    <  1:687641/138‑1 (MQ=255)                                                        aCGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACGTTTTGACCGACTAAGCCAATCATTGTTTCAACCCCTCAATCGCTCAATGACCTGATTAAc                                                                    <  2:264495/138‑1 (MQ=255)                                                         cgcgGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAt                                                                        <  2:357618/133‑1 (MQ=255)                                                         cgcgGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAt                                                                        >  1:357618/1‑133 (MQ=255)                                                         cgcgGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAAcc                                                                   >  1:802952/1‑138 (MQ=255)                                                         cgcgGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAAcc                                                                   >  1:12268/1‑138 (MQ=255)                                                            ggTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAgg                                                               <  2:203292/139‑1 (MQ=255)                                                                tGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGa                                                                              <  1:11890/120‑1 (MQ=255)                                                                tGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGa                                                                              >  2:11890/1‑120 (MQ=255)                                                                tGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAgg                                                               >  1:1197/1‑135 (MQ=255)                                                                tGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAgg                                                               <  2:1197/135‑1 (MQ=255)                                                                tGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGa                                                           >  1:520990/1‑139 (MQ=255)                                                                 gCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGa                                                           <  2:12268/138‑1 (MQ=255)                                                                     cAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       <  2:242979/138‑1 (MQ=255)                                                                     cAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       <  2:658142/138‑1 (MQ=255)                                                                     cAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       <  2:290939/138‑1 (MQ=255)                                                                                       cTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGcc                                    <  2:293496/139‑1 (MQ=255)                                                                                            aTAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       <  1:805249/115‑1 (MQ=255)                                                                                            aTAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTg                                                       >  2:805249/1‑115 (MQ=255)                                                                                            aTAGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATc                               >  2:792206/1‑139 (MQ=255)                                                                                              aGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATc                               >  1:194046/1‑137 (MQ=255)                                                                                              aGAAACGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATc                               >  2:617817/1‑137 (MQ=255)                                                                                                 aaCGCCGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGAc                          >  2:257607/1‑139 (MQ=255)                                                                                                     ccGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAt                                                                        <  2:61326/89‑1 (MQ=255)                                                                                                     ccGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGAt                                                                        >  1:61326/1‑89 (MQ=255)                                                                                                     ccGTCTTCTGTGAAGATACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCAt                      <  2:520990/139‑1 (MQ=255)                                                                                                                    aTACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAc                                                                             <  1:142561/69‑1 (MQ=255)                                                                                                                    aTACGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGAc                                                                             >  2:142561/1‑69 (MQ=255)                                                                                                                       cGGGTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGtttt    >  1:37843/1‑139 (MQ=255)                                                                                                                         ggTCATCCCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGTTTtct  >  2:356145/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                     GGTGGTCACCTGAATAGCACGGTAGCCATCGTTAGCCAGGTCTTTAACCTGAGTAACGCGGTTTGCTTCAACTTCGATTACGGTTACTGGGATAGAAACGCCGTCTTCTGTGAAGATACGGGTCATACCCACTTTTTTACCGACTAAACCAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGATTAACCCAGGCTGATCTGCACGTCTACACCGGCAGCCAGATCCAGACGCATCAGAGCATCAACGGTTTTCT  >  NZ_CP009273/3446130‑3446388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑