Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,479,155	A→G	V8A (GTC→GCC)	yhfA ←	OsmC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,479,155	0	A	G	100.0%	175.4 / NA	51	V8A (GTC→GCC)	yhfA	OsmC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

AACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGACACA  >  NZ_CP009273/3479019‑3479284                                                                                                                                         |                                                                                                                                  aaCCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGcc                                                                                                                                 <  1:459698/139‑1 (MQ=255)      caTCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACtt                                                                                                                            >  2:513452/1‑139 (MQ=255)       aTCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACtt                                                                                                                            >  1:643519/1‑138 (MQ=255)           aTGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTc                                                                                                                       >  2:457865/1‑139 (MQ=255)                    cAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTg                                                                                                                <  1:133025/137‑1 (MQ=255)                      accaccCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtt                                                                                                            <  2:605371/139‑1 (MQ=255)                          cccGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                                                                        <  1:123902/139‑1 (MQ=255)                          cccGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                                                                        >  2:531061/1‑139 (MQ=255)                          cccGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                                                                        >  1:511689/1‑139 (MQ=255)                              ccgccATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTAc                                                                                                    >  1:667718/1‑139 (MQ=255)                                           cATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCaa                                                                                       >  2:497611/1‑139 (MQ=255)                                             tttCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAAtt                                                                                     >  2:70677/1‑139 (MQ=255)                                             tttCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAAtt                                                                                     >  2:536176/1‑139 (MQ=255)                                              ttCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAAttt                                                                                    >  1:799859/1‑139 (MQ=255)                                                  aTCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCt                                                                                <  1:736301/139‑1 (MQ=255)                                                   tCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCt                                                                                >  1:764978/1‑138 (MQ=255)                                                       aCTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACag                                                                           <  2:492740/139‑1 (MQ=255)                                                           ggTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTa                                                                       >  2:295190/1‑139 (MQ=255)                                                           ggTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTa                                                                       >  1:120835/1‑139 (MQ=255)                                                                tttATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtt                                                                                                            <  1:508627/97‑1 (MQ=255)                                                                tttATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtt                                                                                                            >  2:508627/1‑97 (MQ=255)                                                                 ttATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTa                                                                 >  2:529180/1‑139 (MQ=255)                                                                   aTCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtt                                                                                                            >  1:210069/1‑94 (MQ=255)                                                                   aTCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtt                                                                                                            <  2:210069/94‑1 (MQ=255)                                                                       ccTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAAc                                                            >  1:761816/1‑138 (MQ=255)                                                                         tGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCa                                                          >  1:504867/1‑138 (MQ=255)                                                                               gCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATcgc                                                    <  2:267717/138‑1 (MQ=255)                                                                               gCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATcgc                                                    <  1:64195/138‑1 (MQ=255)                                                                                ccGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATcgcg                                                   >  2:507074/1‑138 (MQ=255)                                                                                       ttAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGaa                                           <  2:667718/139‑1 (MQ=255)                                                                                        tAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                          <  1:749016/75‑1 (MQ=255)                                                                                        tAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                          >  2:749016/1‑75 (MQ=255)                                                                                         aaaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAg                                          <  1:25576/138‑1 (MQ=255)                                                                                         aaaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAg                                          <  1:531061/138‑1 (MQ=255)                                                                                         aaaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAg                                          <  1:529180/138‑1 (MQ=255)                                                                                         aaaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAg                                          <  1:756077/138‑1 (MQ=255)                                                                                               tgatgaCCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                                                                        <  2:224832/70‑1 (MQ=255)                                                                                               tgatgaCCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                                                                        >  1:224832/1‑70 (MQ=255)                                                                                                 atgaCCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTGCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGAc                                     <  2:503913/135‑1 (MQ=255)                                                                                                  tgaCGAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTggg                                <  2:336819/139‑1 (MQ=255)                                                                                                              gATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                          <  2:273476/53‑1 (MQ=255)                                                                                                              gATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                          >  1:273476/1‑53 (MQ=255)                                                                                                                ttCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCga                  <  1:536176/139‑1 (MQ=255)                                                                                                                   gCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCgaga                <  1:497611/138‑1 (MQ=255)                                                                                                                          aaaGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGga        <  2:630242/139‑1 (MQ=255)                                                                                                                              ttAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGaca    >  1:348530/1‑139 (MQ=255)                                                                                                                              ttAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGATGAAGCGAGACACCAGGAGaca    >  2:818305/1‑139 (MQ=255)                                                                                                                                aaCCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGacac   <  2:132542/138‑1 (MQ=255)                                                                                                                                 aCCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCACCGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGAcaca  >  1:864488/1‑138 (MQ=255)                                                                                                                                 aCCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGAcaca  <  2:504867/138‑1 (MQ=255)                                                                                                                                 aCCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGAcaca  >  1:502845/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                  AACCACATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGACACA  >  NZ_CP009273/3479019‑3479284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑