| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,511,244 | T→C | E201G (GAA→GGA) | aroB ← | 3‑dehydroquinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,511,244 | 0 | T | C | 81.6% | 105.9 / 22.3 | 49 | E201G (GAA→GGA) | aroB | 3‑dehydroquinate synthase |
| Reads supporting (aligned to +/- strand): ref base T (4/5); new base C (21/19); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-02 | |||||||||||
CAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAG > NZ_CP009273/3511111‑3511364 | cAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTccagc < 2:270600/139‑1 (MQ=255) gATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATGCTCTCccagc > 1:243562/1‑137 (MQ=255) tAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTaaa > 1:23362/1‑138 (MQ=255) gCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACg > 2:568110/1‑139 (MQ=255) aCGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCa < 2:23362/139‑1 (MQ=255) aCGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCa < 1:568110/139‑1 (MQ=255) aCGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCa < 1:464099/139‑1 (MQ=255) aaCCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGt > 2:740589/1‑139 (MQ=255) cgcTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCa > 2:629250/1‑123 (MQ=255) cgcTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCa < 1:629250/123‑1 (MQ=255) cgcTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGc > 1:495000/1‑139 (MQ=255) ccTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGccc < 2:646422/139‑1 (MQ=255) aGTTCACAACAACGGCGAATACAGTACGCCTTTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACg < 2:243562/139‑1 (MQ=255) aGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCa > 2:724035/1‑93 (MQ=255) aGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCa < 1:724035/93‑1 (MQ=255) aGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACg > 1:237061/1‑139 (MQ=255) aacGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCAcg < 1:740589/139‑1 (MQ=255) cGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCAcg > 2:754063/1‑137 (MQ=255) cGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCAcg < 1:754063/137‑1 (MQ=255) cGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTaa < 1:796468/131‑1 (MQ=255) cGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTaa > 2:796468/1‑131 (MQ=255) gCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCggg > 1:805597/1‑139 (MQ=255) tACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAg < 1:500175/139‑1 (MQ=255) tACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCAcg < 1:297216/125‑1 (MQ=255) tACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCAcg > 2:297216/1‑125 (MQ=255) cATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACt > 2:549997/1‑116 (MQ=255) cATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACt < 1:549997/116‑1 (MQ=255) tGCCGGACCGTCCGGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTtga < 2:688297/86‑1 (MQ=255) tGCCGGACCGTCCGGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTtga > 1:688297/1‑86 (MQ=255) tGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAg > 2:285324/1‑139 (MQ=255) tGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAg > 2:770605/1‑139 (MQ=255) gACCGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTc > 2:120662/1‑136 (MQ=255) ccGTCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTc > 2:855837/1‑134 (MQ=255) tCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCa > 1:67225/1‑51 (MQ=255) tCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCa < 2:67225/51‑1 (MQ=255) tCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGa > 2:228479/1‑126 (MQ=255) tCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGa < 1:228479/126‑1 (MQ=255) tCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATcca > 2:756459/1‑139 (MQ=255) tCCAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATcca > 1:509159/1‑139 (MQ=255) ccAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATccac < 2:345515/139‑1 (MQ=255) cAGACGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATccac < 1:95112/138‑1 (MQ=255) aCGCAACAACGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATccaccacc < 1:120662/139‑1 (MQ=255) acaacGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATccacc < 2:865252/131‑1 (MQ=255) acaacGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATccacc > 1:865252/1‑131 (MQ=255) acaacGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTg > 1:142682/1‑138 (MQ=255) aacGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTTATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAg < 2:804022/139‑1 (MQ=255) aacGCATCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAg < 2:302881/139‑1 (MQ=255) aTCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATc > 2:758655/1‑120 (MQ=255) aTCCAGATTCTCTCCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATc < 1:758655/120‑1 (MQ=255) tctcCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAg < 2:253776/124‑1 (MQ=255) tctcCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAg > 1:253776/1‑124 (MQ=255) | CAGATTCAGTAAAGCACGTAACCCGGTTTCGCGCTCGTCGGCGGCGACAACTTCTGCCTTCAGTTCACAACAACGGCGAATACAGTACGCCATTGCCGGACCGTCCAGACGCAACAACGCATCCAGATTCTCTTCCAGCCAGTTAAAAAACGCACCGTCAAGAATAATGCCGTATTTGATGACTTCTGCCAGCCCCGACGCTAACTCACGCGGGGGAAGCGTTTTCAGACAGTCGAGATCCACCACCACTGAAG > NZ_CP009273/3511111‑3511364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |