Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,526,544	C→T	A123V (GCT→GTT)	pckA →	phosphoenolpyruvate carboxykinase (ATP)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,526,544	0	C	T	100.0%	173.1 / NA	45	A123V (GCT→GTT)	pckA	phosphoenolpyruvate carboxykinase (ATP)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/18);  total (27/18)

CTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTA  >  NZ_CP009273/3526409‑3526678                                                                                                                                        |                                                                                                                                       cTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGtttt                                                                                                                                     >  2:232253/1‑139 (MQ=255) cTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGGGACCAAGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGtttt                                                                                                                                     >  1:471519/1‑139 (MQ=255)   cgcgATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCt                                                                                                                                   >  1:180217/1‑139 (MQ=255)    gcgATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCtg                                                                                                                                  <  2:745268/139‑1 (MQ=255)      gATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCtgtg                                                                                                                                >  2:385971/1‑139 (MQ=255)       aTACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGTAAACCTGGCAGCATCTGAAAGGCCGGGTGACCAGGCAGGTTTCCGGCAAACGTCAGTTCGTTGTGGACGTTTTATgggg                                                                                                                               >  2:462103/1‑139 (MQ=255)          cTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTgtggtg                                                                                                                             <  2:823709/138‑1 (MQ=255)            ttCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCg                                                                                                                           >  1:603409/1‑138 (MQ=255)                 gtggGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCg                                                                                                                     <  2:575018/139‑1 (MQ=255)                    ggCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCGGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGa                                                                                                                   >  1:591147/1‑138 (MQ=255)                    ggCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGa                                                                                                                   >  1:660660/1‑138 (MQ=255)                               gcaaaggTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTtc                                                                                                       >  2:710277/1‑139 (MQ=255)                                 aaaggTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTtccg                                                                                                     <  2:699976/139‑1 (MQ=255)                                   aggTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTtccgtc                                                                                                   >  2:234831/1‑139 (MQ=255)                                        aGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGttt                                                                                              >  1:714137/1‑139 (MQ=255)                                               caacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCAcc                                                                                       >  1:24764/1‑139 (MQ=255)                                               caacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCAcc                                                                                       >  1:724365/1‑139 (MQ=255)                                                  caaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGaa                                                                                    <  2:180217/139‑1 (MQ=255)                                                     aCCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTg                                                                                 >  2:527776/1‑139 (MQ=255)                                                       ctctctctCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGc                                                                               >  1:357791/1‑139 (MQ=255)                                                         ctctctcCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCt                                                                             >  1:123591/1‑139 (MQ=255)                                                                 ggAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTccgtccgt                                                                                                >  2:339432/1‑112 (MQ=255)                                                                 ggAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTccgtccgt                                                                                                <  1:339432/112‑1 (MQ=255)                                                                 ggAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGaa                                                                                    >  2:241064/1‑124 (MQ=255)                                                                 ggAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGaa                                                                                    <  1:241064/124‑1 (MQ=255)                                                                          gcagcaTCTGAAAGGCCTGGTGACCAGGCGGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTc                                                            <  2:591147/139‑1 (MQ=255)                                                                             gcaTCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCaaa                                                         <  1:784630/139‑1 (MQ=255)                                                                             gcaTCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCaaa                                                         <  2:72013/139‑1 (MQ=255)                                                                                tCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCaaaaa                                                       >  2:815855/1‑138 (MQ=255)                                                                                         ccTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTAtt                                             <  2:607133/139‑1 (MQ=255)                                                                                           tGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCg                                           >  2:208761/1‑139 (MQ=255)                                                                                            ggTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGc                                          >  1:199994/1‑139 (MQ=255)                                                                                                  cAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGGGc                                    >  2:362837/1‑139 (MQ=255)                                                                                                     gCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAg                                     <  1:830352/135‑1 (MQ=255)                                                                                                     gCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAg                                     >  2:830352/1‑135 (MQ=255)                                                                                                      cAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATg                                <  1:511771/139‑1 (MQ=255)                                                                                                              cGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTg                        >  1:100650/1‑139 (MQ=255)                                                                                                                    aCGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAg                                     <  2:480156/120‑1 (MQ=255)                                                                                                                    aCGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAg                                     >  1:480156/1‑120 (MQ=255)                                                                                                                           ttCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGAt                                 >  1:752101/1‑117 (MQ=255)                                                                                                                           ttCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGAt                                 <  2:752101/117‑1 (MQ=255)                                                                                                                           ttCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAAc           <  2:518625/139‑1 (MQ=255)                                                                                                                                tggcGACGTTTTCTGTGATGCGAACCCGCATAATCGTCTTTCCGTCCGTCTCATCACCGAAATGGACTAGCAAGCGCATTTTGTAAAAAACATGTTTATTCACCCGAGCGATGAAGAACTGGCAGGTTTcaaaccaaac      >  2:768266/4‑139 (MQ=255)                                                                                                                                  tCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACt     <  1:234831/138‑1 (MQ=255)                                                                                                                                   cGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACttt   >  2:651564/1‑139 (MQ=255)                                                                                                                                   cGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACtt    >  2:562762/1‑138 (MQ=255)                                                                                                                                    gACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTa  <  1:446596/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CTCGCGATACTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTA  >  NZ_CP009273/3526409‑3526678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑