Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,589,221	A→G	G181G (GGT→GGC)	livH ←	high‑affinity branched‑chain amino acid ABC transporter permease LivH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,589,221	0	A	G	100.0%	219.9 / NA	63	G181G (GGT→GGC)	livH	high‑affinity branched‑chain amino acid ABC transporter permease LivH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/39);  total (24/39)

ACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAA  >  NZ_CP009273/3589092‑3589354                                                                                                                                  |                                                                                                                                      aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                              >  2:42764/1‑139 (MQ=255) aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                              >  1:786212/1‑139 (MQ=255) aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                              >  1:464494/1‑139 (MQ=255) aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGGCAGCGCAATCACCCGGTCGGTGTTAATGACAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                              >  1:780289/1‑139 (MQ=255) aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGGCAGCGCAAGCACCCGGTCGGTGTTAATGCAAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                              >  2:92256/1‑139 (MQ=255)      gcagcaCACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGaa                                                                                                                           >  2:70405/1‑137 (MQ=255)       cagcaCACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAg                                                                                                                        <  1:124798/139‑1 (MQ=255)               ccGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGa                                                                                                                 >  2:185867/1‑138 (MQ=255)                    aCCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGcc                                                                                                                                     >  1:733742/1‑113 (MQ=255)                    aCCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGcc                                                                                                                                     <  2:733742/113‑1 (MQ=255)                      cgccgcCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTc                                                                                                         <  1:42764/139‑1 (MQ=255)                          gccATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAgcg                                                                                                     <  1:534632/139‑1 (MQ=255)                                gccgcGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGc                                                                                                <  2:419299/138‑1 (MQ=255)                                     gcCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCg                                                                                          <  1:546518/139‑1 (MQ=255)                                       cAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAg                                                                                        <  1:185867/139‑1 (MQ=255)                                          tCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGaa                                                                                     <  1:632237/139‑1 (MQ=255)                                               aaGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTaa                                                                                <  1:470317/139‑1 (MQ=255)                                               aaGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTaa                                                                                <  1:70405/139‑1 (MQ=255)                                                   tCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAAt                                                                            >  2:483760/1‑139 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGaa                                                                                     >  2:602839/1‑128 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGaa                                                                                     <  1:602839/128‑1 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTaa                                                                                <  1:211995/133‑1 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTaa                                                                                <  1:244775/133‑1 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTaa                                                                                >  2:244775/1‑133 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTaa                                                                                >  2:211995/1‑133 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACa                                                                              >  2:341833/1‑135 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACa                                                                              <  1:341833/135‑1 (MQ=255)                                                     aGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATc                                                                           >  1:299970/1‑138 (MQ=255)                                                            tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                   <  1:34490/139‑1 (MQ=255)                                                            tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                   <  1:412563/139‑1 (MQ=255)                                                                   gtcggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGc                                                            <  2:692611/139‑1 (MQ=255)                                                                   gtcggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGc                                                            <  1:483760/139‑1 (MQ=255)                                                                     cggtGTTAATGCCAAGCAGACTCGCCATGTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  2:747201/139‑1 (MQ=255)                                                                      ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACCCGCGCGGCCCAGGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAACCCAGATCCCCGCCTGCAt                                                          <  2:380457/138‑1 (MQ=255)                                                                      ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  1:550160/138‑1 (MQ=255)                                                                      ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  2:299970/138‑1 (MQ=255)                                                                      ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  1:5267/138‑1 (MQ=255)                                                                      ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  1:865602/138‑1 (MQ=255)                                                                      ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  1:630571/138‑1 (MQ=255)                                                                      ggtGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  1:118840/138‑1 (MQ=255)                                                                      ggtGTTAATCCCAAGCATACCCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  2:485118/138‑1 (MQ=255)                                                                        tgtTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCa                                                                                               <  1:609921/99‑1 (MQ=255)                                                                        tgtTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCa                                                                                               >  2:609921/1‑99 (MQ=255)                                                                          ttAATGCCAAGCAGACCCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAtggtg                                                      <  2:722894/138‑1 (MQ=255)                                                                              tGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                   <  1:642965/121‑1 (MQ=255)                                                                              tGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                                   >  2:642965/1‑121 (MQ=255)                                                                                ccAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATag                                               <  2:200262/139‑1 (MQ=255)                                                                                  aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCg                                                                                          >  1:509818/1‑94 (MQ=255)                                                                                  aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCg                                                                                          <  2:509818/94‑1 (MQ=255)                                                                                  aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  2:97434/126‑1 (MQ=255)                                                                                  aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          >  1:97434/1‑126 (MQ=255)                                                                                  aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATagag                                             >  2:528753/1‑139 (MQ=255)                                                                                  aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATagag                                             >  1:690583/1‑139 (MQ=255)                                                                                        aCTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCa                                          >  2:559444/1‑136 (MQ=255)                                                                                                            ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAgg                                                                                       <  2:497577/71‑1 (MQ=255)                                                                                                            ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAgg                                                                                       >  1:497577/1‑71 (MQ=255)                                                                                                            ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          <  2:56091/100‑1 (MQ=255)                                                                                                            ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                          >  1:56091/1‑100 (MQ=255)                                                                                                            ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcc                       <  1:36119/135‑1 (MQ=255)                                                                                                             cgcgCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCccac                   <  1:719150/138‑1 (MQ=255)                                                                                                                   ggCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTc                                                                                                         >  1:297990/1‑46 (MQ=255)                                                                                                                   ggCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTc                                                                                                         <  2:297990/46‑1 (MQ=255)                                                                                                                             gcgcgGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTaa  <  1:559444/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      ACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACTGACCGTTAA  >  NZ_CP009273/3589092‑3589354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑