Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,666,352 A→T T193T (ACA→ACT rcdB → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,666,3520AT81.6% 132.0 / 21.3 51T193T (ACA→ACTrcdBLysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base T (23/17);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGAT  >  NZ_CP009273/3666218‑3666486
                                                                                                                                      |                                                                                                                                      
ccGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCg                                                                                                                                     >  1:439679/1‑138 (MQ=255)
  ggAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGATTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCAc                                                                                                                                  >  2:60519/1‑139 (MQ=255)
    aaGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCt                                                                                                                                <  1:633134/139‑1 (MQ=255)
    aaGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCt                                                                                                                                <  2:283709/139‑1 (MQ=255)
    aaGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCt                                                                                                                                <  2:227202/139‑1 (MQ=255)
    aaGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCt                                                                                                                                <  2:665960/139‑1 (MQ=255)
          gCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGgtg                                                                                                                           >  1:681025/1‑138 (MQ=255)
                       tCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGt                                                                                                             >  1:740011/1‑139 (MQ=255)
                       tCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGt                                                                                                             >  1:287193/1‑139 (MQ=255)
                       tCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGt                                                                                                             >  2:181911/1‑139 (MQ=255)
                                   aCTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                 <  1:181911/139‑1 (MQ=255)
                                   aCTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                 <  2:578919/139‑1 (MQ=255)
                                                 aCTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGt                                                                                   >  2:733709/1‑139 (MQ=255)
                                                     tgCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                                                                               <  2:287193/139‑1 (MQ=255)
                                                      gCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGt                                                                              >  2:511790/1‑139 (MQ=255)
                                                       ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                                                                             >  1:548483/1‑139 (MQ=255)
                                                       ccAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTg                                                                             >  1:199429/1‑139 (MQ=255)
                                                           tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGtt                                                                          >  2:91386/1‑138 (MQ=255)
                                                           tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTg                                                                                                   <  1:412556/113‑1 (MQ=255)
                                                           tCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTg                                                                                                   >  2:412556/1‑113 (MQ=255)
                                                            ccGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGttt                                                                         >  2:426213/1‑138 (MQ=255)
                                                               cACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                 >  1:364001/1‑111 (MQ=255)
                                                               cACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                 <  2:364001/111‑1 (MQ=255)
                                                               cACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                                                                               >  2:502386/1‑129 (MQ=255)
                                                               cACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAg                                                                               <  1:502386/129‑1 (MQ=255)
                                                               cACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGt                                                                     >  2:319922/1‑139 (MQ=255)
                                                                aCTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTc                                                                    <  2:253051/139‑1 (MQ=255)
                                                                            gcTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGtt                                                        <  1:661560/139‑1 (MQ=255)
                                                                             cTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTg                                                       <  1:91386/139‑1 (MQ=255)
                                                                              tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                 >  1:137857/1‑96 (MQ=255)
                                                                              tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGcc                                                                                                 <  2:137857/96‑1 (MQ=255)
                                                                              tttGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGa                                                      >  2:266787/1‑139 (MQ=255)
                                                                                tGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGa                                                      <  2:246838/137‑1 (MQ=255)
                                                                                            aaGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGc                                        >  1:803382/1‑139 (MQ=255)
                                                                                               ccccGATGATCTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGaa                                     <  2:740011/139‑1 (MQ=255)
                                                                                                         cTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTAc                                                                                                                        <  1:115813/46‑1 (MQ=255)
                                                                                                         cTGACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTAc                                                                                                                        >  2:115813/1‑46 (MQ=255)
                                                                                                           gACTTCACACGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCAcc                         >  2:214056/1‑139 (MQ=255)
                                                                                                            aCTTCACACGCAATAGTGCGTTATACCCCGCCCCTGGGTGTACATCCGTTAGGTTTTGAGTTGGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCGGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCAccg                        <  2:225035/139‑1 (MQ=255)
                                                                                                                  cacGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTc                  >  2:796793/1‑139 (MQ=255)
                                                                                                                   acGCAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCt                 >  2:250645/1‑139 (MQ=255)
                                                                                                                      cAATATGGCGTTATACCCCGCTCCTGGGTGTACATCCGTTAGGTTTGGAGTTTGCCAGCGTTAATGGCGTCCATTGGTTTAAGTCTGGCGGCATTTTGACGGAAAACAGTAGCAAAAACTATCTCACCGCCGGTCTTGc              <  1:60519/139‑1 (MQ=255)
                                                                                                                      cAATAGTGGTTTATACTCCGCCCCGGGGTTTACACCCTTTAGGTTTTGAGGTTGCCAGCGTTAAGGGCGTCCAGGGGTTTAAGTCTGGGGGCTTGTTGACGGTAAACAGTAGCAAAAACTATCTCACCGCCGGTCTTGc              <  2:548483/139‑1 (MQ=255)
                                                                                                                      cAATAGTGCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGc              <  1:266787/139‑1 (MQ=255)
                                                                                                                      cAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCGGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGc              <  1:511790/139‑1 (MQ=255)
                                                                                                                            tGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCt        <  1:319922/139‑1 (MQ=255)
                                                                                                                             gCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCa                                                                                >  2:655340/1‑66 (MQ=255)
                                                                                                                             gCGTTATACTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCa                                                                                <  1:655340/66‑1 (MQ=255)
                                                                                                                                 tataCTCCGCACCTGGGTGTACATCCGTTAGGTTATGAGGTTGCCAGCGTTATTGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa   >  1:826592/1‑139 (MQ=255)
                                                                                                                                 tataCACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGa   >  1:365072/1‑139 (MQ=255)
                                                                                                                                   taCTCCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGat  >  2:187738/1‑138 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CCGGAAGATGGCGTTATCGCCCGTCCCCTCGGCAAACTGACCATGGTCAACTGTGCCAGTCCGCACTATCTGACGCGCTTTGGTTATCCGCAAAGCCCCGATGATCTGACTTCACACGCAATAGTGCGTTATACACCGCACCTGGGTGTACATCCGTTAGGTTTTGAGGTTGCCAGCGTTAATGGCGTCCAGTGGTTTAAGTCTGGCGGCATGTTGACGGTAAACAGTAGCGAAAACTATCTCACCGCCGGTCTTGCCGGTCTGGGGAT  >  NZ_CP009273/3666218‑3666486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: