Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,853,103 A→G F125S (TTC→TCC)  yidK ← solute:sodium symporter family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,853,1030AG96.9% 235.2 / ‑1.0 65F125S (TTC→TCC) yidKsolute:sodium symporter family transporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (33/30);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

AGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGA  >  NZ_CP009273/3852968‑3853233
                                                                                                                                       |                                                                                                                                  
aGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAg                                                                                                                                  >  1:839193/1‑138 (MQ=255)
  cAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAg                                                                                                                               <  2:338834/139‑1 (MQ=255)
      aTTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGt                                                                                                                           <  2:452412/139‑1 (MQ=255)
          ccAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCgat                                                                                                                       >  1:294048/1‑139 (MQ=255)
                     aTAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATAcgcg                                                                                                            >  2:511035/1‑139 (MQ=255)
                          cGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGtt                                                                                                       <  2:554071/139‑1 (MQ=255)
                            cACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGt                                                                                                                           <  2:222743/117‑1 (MQ=255)
                            cACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGt                                                                                                                           >  1:222743/1‑117 (MQ=255)
                            cACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGttta                                                                                                     >  2:133096/1‑138 (MQ=255)
                              ccGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTAt                                                                                                   >  1:324887/1‑139 (MQ=255)
                                 tGAGAAATCTGTAGCGATTCCCCGACGTGAAAGAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCa                                                                                                 >  1:514690/1‑138 (MQ=255)
                                 tGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCg                                                                                                                         >  2:451254/1‑114 (MQ=255)
                                 tGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCg                                                                                                                         <  1:451254/114‑1 (MQ=255)
                                 tGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCa                                                                                                 >  1:690191/1‑138 (MQ=255)
                                      aaTCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGt                                                                                           <  2:690191/139‑1 (MQ=255)
                                           gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                      >  2:216513/1‑139 (MQ=255)
                                           gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                      >  2:642750/1‑139 (MQ=255)
                                              gCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                      >  2:563164/1‑136 (MQ=255)
                                              gCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCa                                                                                    <  2:558724/138‑1 (MQ=255)
                                               cGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGa                                                                                  <  1:340261/139‑1 (MQ=255)
                                                 aTTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGaaa                                                                                <  1:642750/139‑1 (MQ=255)
                                                 aTTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGaa                                                                                 >  1:14105/1‑138 (MQ=255)
                                                                    gCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGcgtcg                                                                                                         <  1:472304/95‑1 (MQ=255)
                                                                    gCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGcgtcg                                                                                                         >  2:472304/1‑95 (MQ=255)
                                                                    gCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATg                                                             <  2:294048/139‑1 (MQ=255)
                                                                    gCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATg                                                             >  2:512401/1‑139 (MQ=255)
                                                                        ttGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATACCGTTCCTCCAGAAAATCGGTGATGGTGCCAATGcc                                                           <  2:45580/137‑1 (MQ=255)
                                                                        ttGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           <  2:79197/137‑1 (MQ=255)
                                                                        ttGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           <  1:810218/137‑1 (MQ=255)
                                                                        ttGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           <  1:277440/137‑1 (MQ=255)
                                                                            gcgcCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           >  2:360646/1‑133 (MQ=255)
                                                                            gcgcCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           <  1:360646/133‑1 (MQ=255)
                                                                             cgcCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCtt                                                     >  2:44283/1‑138 (MQ=255)
                                                                                     cgcCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcg                                            <  2:127913/139‑1 (MQ=255)
                                                                                     cgcCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcg                                            <  1:229886/139‑1 (MQ=255)
                                                                                     cgcCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcg                                            <  1:44283/139‑1 (MQ=255)
                                                                                           aGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTaa                                      <  2:14105/139‑1 (MQ=255)
                                                                                                 gaACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGAt                                <  1:404492/139‑1 (MQ=255)
                                                                                                    cAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAg                             >  1:307998/1‑139 (MQ=255)
                                                                                                    cAAACGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGataa                                                                                                                  >  1:750308/1‑54 (MQ=255)
                                                                                                    cAAACGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGataa                                                                                                                  <  2:750308/54‑1 (MQ=255)
                                                                                                     aaTCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGaaact                                                                              >  1:184754/1‑87 (MQ=255)
                                                                                                     aaTCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAAt                                                                              <  2:184754/89‑1 (MQ=255)
                                                                                                     aaTCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgc                            >  2:819170/1‑139 (MQ=255)
                                                                                                     aaTCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgc                            >  1:399046/1‑139 (MQ=255)
                                                                                                     aaTCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgc                            >  1:784686/1‑139 (MQ=255)
                                                                                                     aaTCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgc                            >  1:429758/1‑139 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAg                                                                                                                                  >  1:663123/1‑35 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAg                                                                                                                                  <  2:663123/35‑1 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGt                                                                                                                           <  2:321261/42‑1 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGt                                                                                                                           >  1:321261/1‑42 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           >  1:647549/1‑106 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           <  2:647549/106‑1 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcgc                          >  1:588077/1‑139 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcgc                          >  1:136392/1‑139 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcgc                          >  2:565039/1‑139 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           <  1:195673/106‑1 (MQ=255)
                                                                                                       tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                           >  2:195673/1‑106 (MQ=255)
                                                                                                          gCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcg                           >  1:303994/1‑135 (MQ=255)
                                                                                                          gCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAgcg                           <  2:303994/135‑1 (MQ=255)
                                                                                                                        ccGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCa         <  2:514690/139‑1 (MQ=255)
                                                                                                                          ggTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCgggg                                                                         >  1:280165/1‑73 (MQ=255)
                                                                                                                          ggTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCgggg                                                                         <  2:280165/73‑1 (MQ=255)
                                                                                                                            tGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGc     >  2:21861/1‑139 (MQ=255)
                                                                                                                               cAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCACCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGa  <  2:278927/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
AGCAAAATTACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGA  >  NZ_CP009273/3852968‑3853233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑