Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,866,099 T→C D87G (GAC→GGC)  dgoD ← galactonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,866,0990TC95.7% 155.6 / ‑0.5 46D87G (GAC→GGC) dgoDgalactonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (18/26);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

TCGATAACATCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGTCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGATCCTGACCAATCAAATAGTCACCCAGCTCGTGAACT  >  NZ_CP009273/3865970‑3866227
                                                                                                                                 |                                                                                                                                
tCGATAACATCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCgg                                                                                                                         >  2:142237/1‑139 (MQ=255)
tCGATAACATCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCgg                                                                                                                         >  1:783515/1‑139 (MQ=255)
   aTAACATCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGa                                                                                                                      <  1:422721/139‑1 (MQ=255)
        aTCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGgcg                                                                                                                 <  2:813824/139‑1 (MQ=255)
        aTCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGgcg                                                                                                                 <  1:519493/139‑1 (MQ=255)
        aTCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGgcg                                                                                                                 <  1:554917/139‑1 (MQ=255)
             ccgGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCtcat                                                                                                            <  1:74434/139‑1 (MQ=255)
                            caacccaacTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGAccgcc                                                                                             <  1:74041/139‑1 (MQ=255)
                             aacccaacTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGAccgccg                                                                                            <  2:783515/139‑1 (MQ=255)
                               cccaacTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACcgccgc                                                                                           >  1:657775/1‑138 (MQ=255)
                                   acTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCtcatc                                                                                                           >  2:849403/1‑118 (MQ=255)
                                   acTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCtcatc                                                                                                           <  1:849403/118‑1 (MQ=255)
                                   acTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGa                                                                                         >  1:206467/1‑136 (MQ=255)
                                      gTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAAt                                                                                   >  1:841225/1‑139 (MQ=255)
                                         ggCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCg                                                                                >  2:283466/1‑139 (MQ=255)
                                         ggCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCg                                                                                <  1:142237/139‑1 (MQ=255)
                                          gCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCgg                                                                               >  2:386519/1‑139 (MQ=255)
                                              tAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGgcg                                                                                                                 >  1:175803/1‑101 (MQ=255)
                                              tAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGgcg                                                                                                                 <  2:175803/101‑1 (MQ=255)
                                              tAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCtcat                                                                                                            <  1:168127/106‑1 (MQ=255)
                                              tAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCtcat                                                                                                            >  2:168127/1‑106 (MQ=255)
                                              tAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGgcg                                                                             <  1:156876/137‑1 (MQ=255)
                                              tAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGgcg                                                                             <  1:283466/137‑1 (MQ=255)
                                              tAACTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGgcg                                                                             <  2:542417/137‑1 (MQ=255)
                                                    tGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCAC‑TTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACAt                                                                     >  2:66469/1‑138 (MQ=255)
                                                        gcgAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACt                                                                 <  1:220507/139‑1 (MQ=255)
                                                            aCCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGcc                                                             <  2:841225/139‑1 (MQ=255)
                                                             ccAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGAGGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCa                                                            <  2:348922/139‑1 (MQ=255)
                                                                ggCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCAt                                                           >  1:659336/1‑137 (MQ=255)
                                                                     cccATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGTCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCAt                                                    >  2:771671/1‑139 (MQ=255)
                                                                     cccATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGTCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCAt                                                    >  1:467509/1‑139 (MQ=255)
                                                                      ccATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGAccgc                                                                                              >  2:711287/1‑96 (MQ=255)
                                                                      ccATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGAccgc                                                                                              <  1:711287/96‑1 (MQ=255)
                                                                           aGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATgc                                              <  2:729973/139‑1 (MQ=255)
                                                                               gCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGa                                                                                                                      <  1:689189/63‑1 (MQ=255)
                                                                               gCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGa                                                                                                                      >  2:689189/1‑63 (MQ=255)
                                                                                ccAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGAt                                         <  2:657775/139‑1 (MQ=255)
                                                                                ccAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCGGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGAt                                         <  1:617353/139‑1 (MQ=255)
                                                                                    aCCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGAt                                     >  1:595996/1‑139 (MQ=255)
                                                                                          gcATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGATCCTGAc                               <  2:633974/139‑1 (MQ=255)
                                                                                          gcATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGATCCTGAc                               <  2:100922/139‑1 (MQ=255)
                                                                                                aGCAC‑TTACCTTTGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGATCCTGACCAATCa                         <  1:66469/138‑1 (MQ=255)
                                                                                                             tGATATCCCATAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGATCCTGACCAATCAAATAGTCACCCAg            >  1:248321/1‑139 (MQ=255)
                                                                                                                       tAACGCCTGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGATCCTGACCAATCAAATAGTCACCCAGCTCGTGAACt  >  2:105966/1‑139 (MQ=255)
                                                                                                                              tGGCCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGgcg                                                                             >  1:759413/1‑57 (MQ=255)
                                                                                                                              tGGCCAATCCCGGCGATGGCGCTCATCAGGATCGAACCGCCGCGATAGAATCCGgcg                                                                             <  2:759413/57‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                
TCGATAACATCCGCCGGACGATCGCCGCCAACCCAACTGTAGGCTTTAATTTTGTCGCGAACCAGGCCGCCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCATAACGCCTGGTCAATCCCGGCGATGGCGCTCATCAGGATCGGACCGCCGCGATAGAATCCGGCGCGATACATCACTTGCCATAAGTCATTGATGCGCGATGGATCCTGACCAATCAAATAGTCACCCAGCTCGTGAACT  >  NZ_CP009273/3865970‑3866227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑