| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 361,532 | A→C | L71R (CTG→CGG) | lacI ← | DNA‑binding transcriptional repressor LacI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 361,532 | 0 | A | C | 90.4% | 159.5 / 9.8 | 52 | L71R (CTG→CGG) | lacI | DNA‑binding transcriptional repressor LacI |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (25/22); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01 | |||||||||||
AAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCC > NZ_CP009273/361402‑361666 | aagaTTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAg < 1:126938/139‑1 (MQ=255) tgCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCaa > 1:482063/1‑139 (MQ=255) cACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACg > 1:299624/1‑139 (MQ=255) gccgcTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCaa < 1:305678/139‑1 (MQ=255) gccgcTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCaa > 1:80074/1‑139 (MQ=255) cTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAg > 2:651756/1‑139 (MQ=255) gCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGTGCGTGCAGGGCCCGATTGGAGGCGGCAACGCCAATCAGCAACGACt > 1:856305/1‑139 (MQ=255) aCGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGACCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGttgcc > 1:337883/1‑136 (MQ=255) gcTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGcc < 2:212809/139‑1 (MQ=255) cTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGc > 1:197829/1‑137 (MQ=255) cTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGc > 2:805510/1‑137 (MQ=255) ttCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCaa < 1:752900/110‑1 (MQ=255) ttCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCaa > 2:752900/1‑110 (MQ=255) aTCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCa < 2:795293/139‑1 (MQ=255) tCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAc > 1:66070/1‑139 (MQ=255) tCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAc > 1:636834/1‑139 (MQ=255) accaccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCa < 1:504809/96‑1 (MQ=255) accaccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCa > 2:504809/1‑96 (MQ=255) ccgccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGtt < 1:161668/136‑1 (MQ=255) caccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTg > 1:396974/1‑139 (MQ=255) accacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTgg < 1:117279/139‑1 (MQ=255) accacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTgg < 1:87403/139‑1 (MQ=255) gCACCCAGTTGATCGGCGCGCGATTTAATCGCCGCGACACTTTGCGACGGCGCGTGCAGGGCCCGACTGGCGGTGGCAACGCCAATCAGCAACGACTGCTGGCCCGCCAGTTGTTGTGCCACCCGGTTGGGACTGTact > 2:806212/1‑137 (MQ=255) gCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAt > 2:64846/1‑139 (MQ=255) aGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGcc > 2:764733/1‑102 (MQ=255) aGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGcc < 1:764733/102‑1 (MQ=255) cGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCAt > 1:856713/1‑139 (MQ=255) cGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGAAATTCAGCTCCGCCAt > 2:833530/1‑139 (MQ=255) cgcgAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGGCTGTTTGCCCGcc < 2:27651/92‑1 (MQ=255) cgcgAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGcc > 1:27651/1‑92 (MQ=255) cgcgAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATcgc > 1:841477/1‑139 (MQ=255) cgAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATcgccg > 2:647983/1‑139 (MQ=255) agaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCt > 2:641484/1‑139 (MQ=255) gaTTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCtt < 1:348405/139‑1 (MQ=255) aaTCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACt > 1:31983/1‑139 (MQ=255) ccgcGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGc < 2:388766/59‑1 (MQ=255) ccgcGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGc > 1:388766/1‑59 (MQ=255) ccgcGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTc > 1:338426/1‑139 (MQ=255) cAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGt < 2:651281/139‑1 (MQ=255) cGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCa < 2:841477/139‑1 (MQ=255) cgcgTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcg < 1:478710/73‑1 (MQ=255) cgcgTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcg > 2:478710/1‑73 (MQ=255) gTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGtgg < 1:641484/139‑1 (MQ=255) gTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGtgg < 2:338426/139‑1 (MQ=255) tGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGtggc < 2:724674/139‑1 (MQ=255) tGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGtggc < 2:358847/139‑1 (MQ=255) ggCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGaaa > 1:187116/1‑128 (MQ=255) ggCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGaaa < 2:187116/128‑1 (MQ=255) ggCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGcc < 1:651756/139‑1 (MQ=255) ggCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGcc > 1:550466/1‑139 (MQ=255) gCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcgc > 2:752390/1‑64 (MQ=255) gCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcgc < 1:752390/64‑1 (MQ=255) | AAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCC > NZ_CP009273/361402‑361666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |