| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,963,488 | T→C | intergenic (+51/‑5) | wzzE → / → wecB | ECA polysaccharide chain length modulation protein/UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,963,488 | 0 | T | C | 100.0% | 210.5 / NA | 58 | intergenic (+51/‑5) | wzzE/wecB | ECA polysaccharide chain length modulation protein/UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/28); total (30/28) | |||||||||||
TGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCG > NZ_CP009273/3963356‑3963622 | tGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGAtgt > 1:496900/1‑139 (MQ=255) gCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGAtgt > 1:709523/1‑138 (MQ=255) ccTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGAtgtg < 1:742897/138‑1 (MQ=255) cTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGt < 2:145512/138‑1 (MQ=255) atgatTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTactg < 2:251048/139‑1 (MQ=255) tgatTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGTTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTactga < 1:384795/139‑1 (MQ=255) cATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTAtt > 2:809985/1‑133 (MQ=255) cATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTAtt < 1:809985/133‑1 (MQ=255) cATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTAc > 1:86704/1‑139 (MQ=255) tGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTAcgcg > 1:83965/1‑139 (MQ=255) tGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTAcgcg > 1:36675/1‑139 (MQ=255) ggggCTGAGCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa < 2:150359/139‑1 (MQ=255) ggtcTGATCGGGGCGGGTGACGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa < 2:507680/136‑1 (MQ=255) gggTTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa < 2:516220/139‑1 (MQ=255) gggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa < 1:379990/139‑1 (MQ=255) cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGc > 1:369539/1‑138 (MQ=255) gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTactg > 2:289440/1‑110 (MQ=255) gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTactg < 1:289440/110‑1 (MQ=255) gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCAt > 1:104043/1‑139 (MQ=255) gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCAt > 1:750773/1‑139 (MQ=255) aTCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCAt > 1:196894/1‑138 (MQ=255) ggCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGAt > 2:832937/1‑139 (MQ=255) cTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATgg < 1:513366/139‑1 (MQ=255) cTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATgg < 2:196894/139‑1 (MQ=255) ggTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGgcg < 2:756727/139‑1 (MQ=255) gtttCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGgcgc < 2:215035/136‑1 (MQ=255) tgtCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa < 1:277066/121‑1 (MQ=255) tgtCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa > 2:277066/1‑121 (MQ=255) tCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt > 1:822855/1‑139 (MQ=255) tCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt < 2:822855/139‑1 (MQ=255) gCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTg > 2:180357/1‑139 (MQ=255) gCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTg > 1:289859/1‑139 (MQ=255) aaCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCa > 1:746799/1‑139 (MQ=255) aCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCAt > 1:692259/1‑139 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGa < 1:177536/46‑1 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGa > 2:177536/1‑46 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTg > 2:382926/1‑116 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTg < 1:382926/116‑1 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGc > 1:90135/1‑139 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGc > 2:706802/1‑139 (MQ=255) gcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCt > 2:683821/1‑138 (MQ=255) ggTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTaaa < 2:531426/139‑1 (MQ=255) gTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa > 1:739311/1‑78 (MQ=255) gTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa < 2:739311/78‑1 (MQ=255) gTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAg > 2:60292/1‑139 (MQ=255) aGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGttt > 1:852678/1‑139 (MQ=255) gcgcAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTg < 2:79982/139‑1 (MQ=255) cgcAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGtt > 2:665397/1‑93 (MQ=255) cgcAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGtt < 1:665397/93‑1 (MQ=255) cgcAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCGTTTTTTTGAGGCTAAAGTTTGc > 2:740172/1‑139 (MQ=255) aaGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCa < 1:60292/139‑1 (MQ=255) aaGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCa < 2:709523/139‑1 (MQ=255) gcTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTgcg < 2:104043/139‑1 (MQ=255) cTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTactgac > 2:552608/1‑41 (MQ=255) cTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTactgac < 1:552608/41‑1 (MQ=255) cTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTgcg > 1:405113/1‑138 (MQ=255) agagAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCt < 2:852678/139‑1 (MQ=255) agaACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCg < 2:134341/139‑1 (MQ=255) | TGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCG > NZ_CP009273/3963356‑3963622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |