Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,052,334	A→G	I190V (ATT→GTT)	typA →	ribosome‑dependent GTPase TypA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,052,334	0	A	G	100.0%	211.5 / NA	59	I190V (ATT→GTT)	typA	ribosome‑dependent GTPase TypA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/27);  total (32/27)

ATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGCCGAAC  >  NZ_CP009273/4052198‑4052468                                                                                                                                         |                                                                                                                                       aTTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGtt                                                                                                                                      >  2:283606/1‑139 (MQ=255) aTTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGtt                                                                                                                                      >  1:847653/1‑139 (MQ=255)   tCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCTCCTGGACCAGGCGGttgt                                                                                                                                    >  2:289245/1‑139 (MQ=255)                     tCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTTTTGACCACGTTCCTGCGcc                                                                                                                  >  2:760268/1‑139 (MQ=255)                     tCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGcc                                                                                                                  >  2:502598/1‑139 (MQ=255)                        aCGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGa                                                                                                               >  2:296339/1‑139 (MQ=255)                            gaccgacGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGtt                                                                                                           <  2:810751/139‑1 (MQ=255)                              ccgacGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGa                                                                                                         <  1:313143/139‑1 (MQ=255)                                    agcagcTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGa                                                                                                   <  1:695877/139‑1 (MQ=255)                                        gcTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGcc                                                                                                                  <  1:298594/120‑1 (MQ=255)                                        gcTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGcc                                                                                                                  >  2:298594/1‑120 (MQ=255)                                        gcTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGt                                                                                               <  1:158659/139‑1 (MQ=255)                                                 cccGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTc                                                                                         >  1:813551/1‑136 (MQ=255)                                                 cccGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTc                                                                                         >  2:707987/1‑136 (MQ=255)                                                      tCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCa                                                                                 <  1:92118/139‑1 (MQ=255)                                                         tttACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCCGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGAt                                                                              >  2:142263/1‑139 (MQ=255)                                                           tACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGAttt                                                                            >  2:392476/1‑139 (MQ=255)                                                              gCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTtctc                                                                         <  2:260008/139‑1 (MQ=255)                                                                  cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                                      <  2:454344/138‑1 (MQ=255)                                                                  cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCt                                                                     >  1:410570/1‑139 (MQ=255)                                                                  cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGATGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCt                                                                     >  1:314201/1‑139 (MQ=255)                                                                   tGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTc                                                                    >  1:398358/1‑139 (MQ=255)                                                                     cgcTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTc                                                                    <  2:813551/137‑1 (MQ=255)                                                                          aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTCCCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTa                                                               <  2:807702/137‑1 (MQ=255)                                                                          aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              <  2:439233/138‑1 (MQ=255)                                                                          aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              >  1:329970/1‑138 (MQ=255)                                                                          aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              <  2:398358/138‑1 (MQ=255)                                                                              gTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAg                                                         <  1:521527/139‑1 (MQ=255)                                                                              gTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAg                                                         <  2:410570/139‑1 (MQ=255)                                                                              gTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAg                                                         <  2:542901/139‑1 (MQ=255)                                                                              gTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGATGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAg                                                         <  2:314201/139‑1 (MQ=255)                                                                              gTATCGCGGGTCTGGACCACGAAGATATGGCGAAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAg                                                         <  1:289245/139‑1 (MQ=255)                                                                                   gcgGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATg                                                    <  1:392476/139‑1 (MQ=255)                                                                                    cgGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGt                                                   >  2:51335/1‑139 (MQ=255)                                                                                       gTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTgg                                                >  2:220341/1‑139 (MQ=255)                                                                                         cTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTTACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCg                                              >  2:821353/1‑139 (MQ=255)                                                                                         cTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCg                                              >  1:692435/1‑139 (MQ=255)                                                                                             aCCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTAt                                          <  2:847653/139‑1 (MQ=255)                                                                                                 cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  1:102022/1‑138 (MQ=255)                                                                                                 cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  2:800386/1‑138 (MQ=255)                                                                                                 cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  2:520836/1‑138 (MQ=255)                                                                                                 cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  1:44775/1‑138 (MQ=255)                                                                                                 cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGc                                      <  1:142263/139‑1 (MQ=255)                                                                                                 cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGCTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  2:7149/1‑138 (MQ=255)                                                                                                 cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGATGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  1:87514/1‑138 (MQ=255)                                                                                                  gAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCa                                     <  1:707987/139‑1 (MQ=255)                                                                                                           gCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCa                            <  1:220341/139‑1 (MQ=255)                                                                                                               aaGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCaa                        <  2:692435/139‑1 (MQ=255)                                                                                                                aGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAATAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAg                       <  1:488893/139‑1 (MQ=255)                                                                                                                  aCATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAgcg                     <  2:693987/139‑1 (MQ=255)                                                                                                                     tGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCgg                  >  1:163329/1‑139 (MQ=255)                                                                                                                           cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGt                                                                                               >  1:511647/1‑56 (MQ=255)                                                                                                                           cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGt                                                                                               <  2:511647/56‑1 (MQ=255)                                                                                                                           cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGt            >  1:187879/1‑139 (MQ=255)                                                                                                                           cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGt            >  1:799560/1‑139 (MQ=255)                                                                                                                            gCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGt            >  1:542577/1‑138 (MQ=255)                                                                                                                             cTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTAt                                          >  2:773267/1‑107 (MQ=255)                                                                                                                             cTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTAt                                          <  1:773267/107‑1 (MQ=255)                                                                                                                                     ggCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGCCGAAc  >  1:140066/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       ATTCGATCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGCCGAAC  >  NZ_CP009273/4052198‑4052468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑