Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,132,297	T→C	intergenic (‑148/‑161)	ptsP ← / → frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,132,297	0	T	C	100.0%	118.4 / NA	34	intergenic (‑148/‑161)	ptsP/frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/13);  total (21/13)

GTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATG  >  NZ_CP009273/4132163‑4132431                                                                                                                                       |                                                                                                                                       gTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCgg                                                                                                                                    >  1:369752/1‑139 (MQ=255) gTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATG‑AATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGt                                                                                                                                   >  1:340004/1‑139 (MQ=255)        tttCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCg                                                                                                                             >  2:469327/1‑139 (MQ=255)          tCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGc                                                                                                                            <  2:433021/138‑1 (MQ=255)              tttACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGt                                                                                                                        >  1:704229/1‑138 (MQ=255)                 aCAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTAt                                                                                                                     >  2:773064/1‑138 (MQ=255)                        aaGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGa                                                                                                             >  2:2114/1‑139 (MQ=255)                               cACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCAtt                                                                                                      >  2:35567/1‑139 (MQ=255)                               cACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCAtt                                                                                                      >  2:321404/1‑139 (MQ=255)                                    cAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGt                                                                                                 <  2:721132/139‑1 (MQ=255)                                          ccTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCAttt                                                                                                     >  2:637616/1‑129 (MQ=255)                                          ccTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCAttt                                                                                                     <  1:637616/129‑1 (MQ=255)                                          ccTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGa                                                                                            >  2:272151/1‑138 (MQ=255)                                                aTGTGACAAATCTGCCAAAAGCTGGACAAATG‑AATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                                    <  2:340004/139‑1 (MQ=255)                                                 tgtgACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                                    <  1:35567/139‑1 (MQ=255)                                                             gCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTaaa                                                                        >  2:660169/1‑139 (MQ=255)                                                                                  atgtaaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                                    >  2:97095/1‑106 (MQ=255)                                                                                  atgtaaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                                    <  1:97095/106‑1 (MQ=255)                                                                                     taaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCt                                                <  1:321404/139‑1 (MQ=255)                                                                                       aCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATg                                                                                                                    <  2:38364/69‑1 (MQ=255)                                                                                       aCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATg                                                                                                                    >  1:38364/1‑69 (MQ=255)                                                                                       aCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTcc                                              >  2:352212/1‑139 (MQ=255)                                                                                              aTTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCg                                       <  1:2114/139‑1 (MQ=255)                                                                                               tttGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGa                                      <  2:352451/139‑1 (MQ=255)                                                                                                  gCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGAtt                                    <  2:235236/138‑1 (MQ=255)                                                                                                          tctcACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTa                                                                                                <  1:669628/70‑1 (MQ=255)                                                                                                          tctcACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTa                                                                                                >  2:669628/1‑70 (MQ=255)                                                                                                                 aGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTtcctcc                    <  2:397813/139‑1 (MQ=255)                                                                                                                   aCGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCAt                  >  1:818294/1‑139 (MQ=255)                                                                                                                     gCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATc                 >  2:386360/1‑138 (MQ=255)                                                                                                                              cGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATg        >  1:121550/1‑138 (MQ=255)                                                                                                                                   tGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATg  >  1:330373/1‑139 (MQ=255)                                                                                                                                   tGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATg  >  1:541788/1‑139 (MQ=255)                                                                                                                                   tGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATg  >  1:272359/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       GTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATG  >  NZ_CP009273/4132163‑4132431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑