Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,223,121	A→G	V14A (GTA→GCA)	lysC ←	lysine‑sensitive aspartokinase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,223,121	0	A	G	100.0%	202.9 / NA	54	V14A (GTA→GCA)	lysC	lysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/34);  total (20/34)

GTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTT  >  NZ_CP009273/4222993‑4223252                                                                                                                                 |                                                                                                                                    gTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTAcc                                                                                                                           >  2:505469/1‑139 (MQ=255)    ccAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGc                                                                                                                         <  1:404061/138‑1 (MQ=255)             cAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTg                                                                                                                 >  2:331071/1‑137 (MQ=255)              aGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCTAAATCAGCTGCGCTGGTACCGCCAAATTTGga                                                                                                               <  1:743160/138‑1 (MQ=255)                cTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGac                                                                                                            <  2:639262/139‑1 (MQ=255)                cTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGac                                                                                                            <  2:334961/139‑1 (MQ=255)                    aGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGacaac                                                                                                         >  2:666050/1‑138 (MQ=255)                          cagcagATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAACTGCGCTGGTACCGCCAAATTTGGAGACAACAAttt                                                                                                    >  1:99848/1‑137 (MQ=255)                            gcagATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGa                                                                                                <  2:689225/139‑1 (MQ=255)                            gcagATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGa                                                                                                <  1:505469/139‑1 (MQ=255)                                aTTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATa                                                                                            <  1:820948/139‑1 (MQ=255)                                     tGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGc                                                                                                                         >  1:450099/1‑105 (MQ=255)                                     tGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGc                                                                                                                         <  2:450099/105‑1 (MQ=255)                                       aTACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTAcc                                                                                      <  2:524994/138‑1 (MQ=255)                                       aTACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCt                                                                                     >  1:449628/1‑139 (MQ=255)                                       aTACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCt                                                                                     >  2:528171/1‑139 (MQ=255)                                         aCCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCg                                                                                   >  1:318217/1‑139 (MQ=255)                                          ccAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGa                                                                                                >  1:603554/1‑125 (MQ=255)                                          ccAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGa                                                                                                <  2:603554/125‑1 (MQ=255)                                             gcagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTc                                                                               <  2:50586/139‑1 (MQ=255)                                             gcagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTc                                                                               <  2:99848/139‑1 (MQ=255)                                              cagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTAc                                                                                       >  1:298727/1‑130 (MQ=255)                                              cagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTAc                                                                                       <  2:298727/130‑1 (MQ=255)                                               agAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAg                                                                             <  1:666050/139‑1 (MQ=255)                                                   gCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGa                                                                         >  1:851393/1‑139 (MQ=255)                                                     cGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATc                                                                       <  1:807046/139‑1 (MQ=255)                                                     cGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATc                                                                       <  2:804889/139‑1 (MQ=255)                                                                     cGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTgg                                                        >  2:849647/1‑138 (MQ=255)                                                                      gCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGca                                                      <  1:799126/139‑1 (MQ=255)                                                                      gCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGca                                                      <  1:117206/139‑1 (MQ=255)                                                                          gTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAg                                                  >  1:517179/1‑139 (MQ=255)                                                                                 tCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAagag                                           <  2:77249/139‑1 (MQ=255)                                                                                 tCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAagag                                           <  2:353732/139‑1 (MQ=255)                                                                                      aaGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGaa                                      <  1:636894/139‑1 (MQ=255)                                                                                        gCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGa                                    <  1:330347/139‑1 (MQ=255)                                                                                                  aGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcg                          <  2:318217/139‑1 (MQ=255)                                                                                                  aGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcg                          <  1:714463/139‑1 (MQ=255)                                                                                                  aGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcg                          <  1:528171/139‑1 (MQ=255)                                                                                                   gcgcTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcg                          >  2:177925/1‑138 (MQ=255)                                                                                                        gCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGc                    >  2:186521/1‑139 (MQ=255)                                                                                                         cGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGa                                                                                                <  1:388790/62‑1 (MQ=255)                                                                                                         cGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGa                                                                                                >  2:388790/1‑62 (MQ=255)                                                                                                         cGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAagag                                           <  2:777808/115‑1 (MQ=255)                                                                                                         cGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAagag                                           >  1:777808/1‑115 (MQ=255)                                                                                                         cGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGc                    >  1:628573/1‑138 (MQ=255)                                                                                                               aTGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACtt             <  1:80179/139‑1 (MQ=255)                                                                                                                 ggCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCa                                                                                                  >  2:739460/1‑52 (MQ=255)                                                                                                                 ggCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCa                                                                                                  <  1:739460/52‑1 (MQ=255)                                                                                                                    gTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGcta        <  2:323067/139‑1 (MQ=255)                                                                                                                      cAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGctac       >  1:128913/1‑138 (MQ=255)                                                                                                                          aTCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACGAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTAttt  <  2:445158/139‑1 (MQ=255)                                                                                                                          aTCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTAttt  <  1:467366/139‑1 (MQ=255)                                                                                                                          aTCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTAttt  <  2:559424/139‑1 (MQ=255)                                                                                                                          aTCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACCTCGCTACTAttt  <  1:331071/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    GTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTT  >  NZ_CP009273/4222993‑4223252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑