Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,235,276 T→C N358D (AAC→GAC)  malE ← maltose/maltodextrin ABC transporter substrate‑binding protein MalE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,235,2760TC94.7% 118.6 / ‑0.1 38N358D (AAC→GAC) malEmaltose/maltodextrin ABC transporter substrate‑binding protein MalE
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (21/15);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

ACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGACCTTCATC  >  NZ_CP009273/4235151‑4235411
                                                                                                                             |                                                                                                                                       
acaGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTc                                                                                                                             <  1:125766/138‑1 (MQ=255)
  aGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCAcc                                                                                                                          >  2:132886/1‑139 (MQ=255)
       tACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCt                                                                                                                     >  1:137526/1‑139 (MQ=255)
             gTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCg                                                                                                                <  1:200762/138‑1 (MQ=255)
              tGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGtt                                                                                                              >  1:145275/1‑139 (MQ=255)
              tGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGtt                                                                                                              <  2:145275/139‑1 (MQ=255)
              tGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGtt                                                                                                              >  2:321097/1‑139 (MQ=255)
                       tCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCAtggt                                                                                                     >  2:46688/1‑139 (MQ=255)
                           cgcgTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTggcg                                                                                                 <  2:286309/139‑1 (MQ=255)
                                     aGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTg                                                                                       <  1:321097/139‑1 (MQ=255)
                                          ttCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCt                                                                                  >  2:468389/1‑139 (MQ=255)
                                            cATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCt                                                                                  <  2:427023/137‑1 (MQ=255)
                                                    gTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCACCAAct                                                                        >  1:230384/1‑139 (MQ=255)
                                                              cGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                                               >  2:153843/1‑138 (MQ=255)
                                                               gCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                                               >  1:159056/1‑137 (MQ=255)
                                                                       cgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                                     <  1:509393/139‑1 (MQ=255)
                                                                                   cAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAcc                                         >  2:652944/1‑139 (MQ=255)
                                                                                     gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCa                                       <  2:432091/139‑1 (MQ=255)
                                                                                      tACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                                     <  1:663177/108‑1 (MQ=255)
                                                                                      tACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                                     >  2:663177/1‑108 (MQ=255)
                                                                                               cATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc                             >  1:646143/1‑139 (MQ=255)
                                                                                                aTACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgcg                                                   >  1:372042/1‑116 (MQ=255)
                                                                                                aTACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgcg                                                   <  2:372042/116‑1 (MQ=255)
                                                                                                  aCCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCg                                                                                                                >  2:253651/1‑53 (MQ=255)
                                                                                                  aCCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCg                                                                                                                <  1:253651/53‑1 (MQ=255)
                                                                                                  aCCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                                     <  2:440516/112‑1 (MQ=255)
                                                                                                  aCCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                                     >  1:440516/1‑112 (MQ=255)
                                                                                                         aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc                   >  2:316714/1‑139 (MQ=255)
                                                                                                         aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc                   >  2:450232/1‑139 (MQ=255)
                                                                                                         aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc                   <  1:760517/139‑1 (MQ=255)
                                                                                                         aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc                   >  2:527307/1‑139 (MQ=255)
                                                                                                           cGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGc                 >  2:505511/1‑139 (MQ=255)
                                                                                                              aCATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                                  <  1:840063/87‑1 (MQ=255)
                                                                                                              aCATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                                  >  2:840063/1‑87 (MQ=255)
                                                                                                                     cGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGAc         <  2:646143/137‑1 (MQ=255)
                                                                                                                          tGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGTTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGACCTtcatc  <  2:483377/139‑1 (MQ=255)
                                                                                                                          tGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGACCTtcat   >  1:174455/1‑138 (MQ=255)
                                                                                                                          tGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGACCTtcat   >  2:700202/1‑138 (MQ=255)
                                                                                                                             |                                                                                                                                       
ACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGACCTTCATC  >  NZ_CP009273/4235151‑4235411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑