Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,262,930	A→T	D290E (GAT→GAA)	uvrA ←	excinuclease ABC subunit UvrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,262,930	0	A	T	100.0%	174.1 / NA	45	D290E (GAT→GAA)	uvrA	excinuclease ABC subunit UvrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/23);  total (22/23)

ACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAG  >  NZ_CP009273/4262805‑4263059                                                                                                                              |                                                                                                                                  aCGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCt                                                                                                                      >  1:257615/1‑139 (MQ=255)  cGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTg                                                                                                                     <  1:479169/139‑1 (MQ=255)   gTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGt                                                                                                                    <  2:484599/139‑1 (MQ=255)                  aTCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGcc                                                                                                               >  2:527783/1‑129 (MQ=255)                  aTCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGcc                                                                                                               <  1:527783/129‑1 (MQ=255)                  aTCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCg                                                                                                     >  1:151480/1‑139 (MQ=255)                  aTCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCg                                                                                                     >  2:535424/1‑139 (MQ=255)                  aTCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCg                                                                                                     >  1:830491/1‑139 (MQ=255)                  aTCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCg                                                                                                     >  1:773227/1‑139 (MQ=255)                    cTGACAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCg                                                                                                     >  1:9930/1‑137 (MQ=255)                       ccAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAata                                                                                                                           <  2:866351/112‑1 (MQ=255)                       ccAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAata                                                                                                                           >  1:866351/1‑112 (MQ=255)                       ccAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGc                                                                                                    <  1:294902/135‑1 (MQ=255)                       ccAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAgg                                                                                                 <  1:252896/138‑1 (MQ=255)                          gCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCgg                                                                                             >  2:376173/1‑139 (MQ=255)                                                 taGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTaaa                                                                     >  2:232465/1‑139 (MQ=255)                                                    aaGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGa                                                                  <  1:804842/139‑1 (MQ=255)                                                         gcggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGaaaa                                                               >  2:321338/1‑137 (MQ=255)                                                          cggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAgg                                                                                                 <  2:426068/103‑1 (MQ=255)                                                          cggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAgg                                                                                                 >  1:426068/1‑103 (MQ=255)                                                                        aCGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGtt                                              <  2:847242/139‑1 (MQ=255)                                                                           gATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCAc                                           >  2:731073/1‑139 (MQ=255)                                                                            aTCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCGGTCGCAGGTCGGGGAGG‑CCGCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg                                          <  2:357621/139‑1 (MQ=255)                                                                            aTCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg                                          <  2:773227/139‑1 (MQ=255)                                                                               gCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa                                        <  1:540817/138‑1 (MQ=255)                                                                                 tccaccaGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGTTTCGAAACAGTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa                                        <  2:54009/135‑1 (MQ=255)                                                                                     ccagccagCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCg                                                                                                                                <  1:842555/45‑1 (MQ=255)                                                                                     ccagccagCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCg                                                                                                                                >  2:842555/1‑45 (MQ=255)                                                                                        gccagcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTGGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGt                                                           <  2:50366/110‑1 (MQ=255)                                                                                        gccagcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGt                                                           >  1:50366/1‑110 (MQ=255)                                                                                        gccagcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGcc                              >  2:56032/1‑139 (MQ=255)                                                                                                  gTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTggg                    <  1:747531/139‑1 (MQ=255)                                                                                                   ttCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGtt                                              <  1:504743/112‑1 (MQ=255)                                                                                                   ttCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGtt                                              >  2:504743/1‑112 (MQ=255)                                                                                                          ttCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCAAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGaa            <  1:1852/139‑1 (MQ=255)                                                                                                             tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTcc                                                  >  1:249935/1‑98 (MQ=255)                                                                                                             tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTcc                                                  <  2:249935/98‑1 (MQ=255)                                                                                                             tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGtt         >  2:657332/1‑139 (MQ=255)                                                                                                               gATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTgg       >  1:118001/1‑139 (MQ=255)                                                                                                                 tCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCg     <  2:151480/139‑1 (MQ=255)                                                                                                                    cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt                                                    <  2:298620/89‑1 (MQ=255)                                                                                                                    cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt                                                    >  1:298620/1‑89 (MQ=255)                                                                                                                    cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGgag  <  1:710882/139‑1 (MQ=255)                                                                                                                       gATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg                                          >  2:763237/1‑96 (MQ=255)                                                                                                                       gATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg                                          <  1:763237/96‑1 (MQ=255)                                                                                                                              |                                                                                                                                  ACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAG  >  NZ_CP009273/4262805‑4263059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑