Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 453,695 G→A A272T (GCG→ACG)  clpX → ATP‑dependent protease ATP‑binding subunit ClpX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273453,6950GA100.0% 117.5 / NA 32A272T (GCG→ACG) clpXATP‑dependent protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/11);  total (21/11)

GGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAAT  >  NZ_CP009273/453561‑453826
                                                                                                                                      |                                                                                                                                   
ggCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGGTTTGCCGGGCTGGGTAAAAGGATTTCCCACCGGGTAGAAAACGGCGCCGGCATTGGTTTTTGcacg                                                                                                                                   >  1:85143/1‑137 (MQ=255)
                      aTTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTGCCCGGCTGGGTAAAAAGATTTTCCACCGCGGAAAAACCGGCCCCGGAAATGGTTTTGGCACGACGGGAAAAACGGAGTCCGACa                                                                                                             >  1:221621/1‑137 (MQ=255)
                         cTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAaagc                                                                                                         >  1:81859/1‑138 (MQ=255)
                                   gATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTTCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCCTTGGTTTTTGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGc                                                                                               >  1:21526/1‑138 (MQ=255)
                                          ctAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCCTTGGGTTTGGCACGACGGGAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAgctgct                                                                                       >  1:130479/1‑139 (MQ=255)
                                              gATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGgcg                                                                                   <  2:781829/139‑1 (MQ=255)
                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGATTTTCCACCGTGTAGAAAACGGCTCCCGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGGGCTGCTGGGGCAgg                                                                               >  2:448309/1‑139 (MQ=255)
                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCCCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAAGCCGACAAAGCAAGCGAAGGCGGGCTGCTGGGGCAgg                                                                               >  1:431272/1‑139 (MQ=255)
                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAATGATTTCCCACCGGGTAGAAAACGGCTCCGGCGTTGGTTTTGGCACGGCGGTAAAAGCGAAGTCCGACAAAACAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                               >  2:741672/1‑139 (MQ=255)
                                                  cTGTTTATTTGTGGCGGTGCGTTTGCCGGGCTGGGTAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGGACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAgg                                                                               >  2:218771/1‑139 (MQ=255)
                                                   tGTTTATTTGTGGCGGTGCGGTTGCCGGTCTGGATAAAGTGATTTTCCACCGTGTAGAAACCGGCTCCGGCATTGGGTTTGGCACGACGGGAAAAGCGAAGGCCGACAAAGCAAGCGAAGGGGAGCTGCTGGCGCAAGt                                                                              >  2:402268/1‑139 (MQ=255)
                                                   tGTTTATTTGTGGCGGTGCGGTTGCCGGTCTGGATAAAGTGATTTTCCACCGTGTAGAAAACGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGt                                                                              >  2:291828/1‑139 (MQ=255)
                                                             tgGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGa                                                                     <  2:248029/138‑1 (MQ=255)
                                                               gCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAg                                                                   <  2:794058/138‑1 (MQ=255)
                                                                                cTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTc                                                 >  2:593720/1‑139 (MQ=255)
                                                                                   gATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCt                                                <  1:291828/137‑1 (MQ=255)
                                                                                   gATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTa                                              <  1:218547/139‑1 (MQ=255)
                                                                                        aGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCt                                         >  2:179911/1‑139 (MQ=255)
                                                                                             tttCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGCCGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGtt                                    >  2:62618/1‑139 (MQ=255)
                                                                                             tttCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGtt                                    >  1:75742/1‑139 (MQ=255)
                                                                                                    ccGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAgctg                                                                                         <  1:297262/79‑1 (MQ=255)
                                                                                                    ccGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAgctg                                                                                         >  2:297262/1‑79 (MQ=255)
                                                                                                      gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            >  1:731513/1‑106 (MQ=255)
                                                                                                      gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            <  2:731513/106‑1 (MQ=255)
                                                                                                      gtgtAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGgtcg                           >  2:136628/1‑139 (MQ=255)
                                                                                                         tAGAAACCGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCt                        >  1:131507/1‑139 (MQ=255)
                                                                                                                cGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTAt                                 <  2:29668/123‑1 (MQ=255)
                                                                                                                cGGCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTAt                                 >  1:29668/1‑123 (MQ=255)
                                                                                                                 ggCTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTg                >  1:694301/1‑139 (MQ=255)
                                                                                                                   cTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            <  2:445834/93‑1 (MQ=255)
                                                                                                                   cTCCGGCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGAt                                                            >  1:445834/1‑93 (MQ=255)
                                                                                                                        gCATTGGTTTTGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAAc         <  1:218771/139‑1 (MQ=255)
                                                                                                                               ttttGGCACGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtgaat  <  2:75742/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGGTAAAAGCGAAGTCCGACAAAGCAAGCGAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAAT  >  NZ_CP009273/453561‑453826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑