| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,309,246 | T→C | I60V (ATT→GTT) | phnJ ← | alpha‑D‑ribose 1‑methylphosphonate 5‑phosphate C‑P‑lyase PhnJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,309,246 | 0 | T | C | 78.8% | 101.2 / 27.7 | 52 | I60V (ATT→GTT) | phnJ | alpha‑D‑ribose 1‑methylphosphonate 5‑phosphate C‑P‑lyase PhnJ |
| Reads supporting (aligned to +/- strand): ref base T (7/4); new base C (24/17); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-04 | |||||||||||
ATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCG > NZ_CP009273/4309113‑4309372 | aTAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGc < 2:757593/139‑1 (MQ=255) cGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCg < 2:389817/139‑1 (MQ=255) tCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCgg > 2:741365/1‑138 (MQ=255) gCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGt > 1:89938/1‑137 (MQ=255) gCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGt > 2:722212/1‑137 (MQ=255) ccGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCgg < 1:57138/138‑1 (MQ=255) ccGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGt > 1:124570/1‑139 (MQ=255) ccGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGt > 1:682144/1‑139 (MQ=255) ccGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGt > 2:421573/1‑139 (MQ=255) tttACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGc > 1:131694/1‑106 (MQ=255) tttACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGc < 2:131694/106‑1 (MQ=255) ggTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTAcgg > 2:644614/1‑138 (MQ=255) tGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCAtctc > 1:342540/1‑139 (MQ=255) gTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTa < 1:514460/117‑1 (MQ=255) gTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTa > 2:514460/1‑117 (MQ=255) gTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGcgcccg > 1:686516/1‑139 (MQ=255) tCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACgg < 2:724714/139‑1 (MQ=255) gACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCa < 2:686516/139‑1 (MQ=255) ttGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAAc < 2:682144/139‑1 (MQ=255) gtCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGc > 2:697432/1‑53 (MQ=255) gtCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGc < 1:697432/53‑1 (MQ=255) gtCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTa < 1:608027/90‑1 (MQ=255) gtCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTa > 2:608027/1‑90 (MQ=255) gtCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATc > 2:325475/1‑139 (MQ=255) cATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCg < 1:660570/131‑1 (MQ=255) cATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCg > 2:660570/1‑131 (MQ=255) aTCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTc > 1:84429/1‑77 (MQ=255) aTCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTc < 2:84429/77‑1 (MQ=255) tCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCa < 1:576589/139‑1 (MQ=255) cgcgCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCAcc < 2:390515/139‑1 (MQ=255) tCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGc > 1:405740/1‑101 (MQ=255) tCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGc < 2:405740/101‑1 (MQ=255) tCACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACC‑‑‑GATCGCCACCGCTTTTAAGATGGcgcg > 2:684290/1‑139 (MQ=255) cACCTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGgcg > 1:256792/1‑139 (MQ=255) ccTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTc > 1:106134/1‑57 (MQ=255) ccTTCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTc < 2:106134/57‑1 (MQ=255) ttCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCgcggc > 2:864120/1‑139 (MQ=255) ttCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACgg > 2:336360/1‑95 (MQ=255) ttCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACgg < 1:336360/95‑1 (MQ=255) ttCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCgcggc > 2:289539/1‑139 (MQ=255) ttCAGCACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGCCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCgcggc > 1:793212/1‑139 (MQ=255) cACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCAc < 2:193143/114‑1 (MQ=255) cACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCAc > 1:193143/1‑114 (MQ=255) cACGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGAt > 2:706631/1‑138 (MQ=255) aCGTCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCa < 2:89938/139‑1 (MQ=255) tCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGgc > 2:330163/1‑125 (MQ=255) tCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGgc < 1:330163/125‑1 (MQ=255) tCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGc > 1:775210/1‑139 (MQ=255) tCGCTTTCGCCAACCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGc > 1:32542/1‑139 (MQ=255) cGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCAc > 2:650536/1‑109 (MQ=255) cGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCAc < 1:650536/109‑1 (MQ=255) cGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCg > 1:237151/1‑139 (MQ=255) | ATAACCGTCGCATCGTCCGTACGTTCCGTGGTGTTTACCCCGGTCACGCGCTTGAAGAAGTTGCGAATCGACACGGCGTTGGTGGTGTCATCCGCGCCCTGGTCAATCACCTTCAGCACGTCGCTTTCGCCAATCACGCTGGCGGTAAGCTGAATGCCGCCGGTTCCCCAGCCGTACGGCATCGGCATCTCGCGCCCGCCAAACGGCACCTGATAACCGGGGATCGCCACCGCTTTTAAGATGGCGCGGCGGATCATGCG > NZ_CP009273/4309113‑4309372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |