Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,321,803	T→C	H495H (CAT→CAC)	proP →	glycine betaine/L‑proline transporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,321,803	0	T	C	100.0%	133.9 / NA	38	H495H (CAT→CAC)	proP	glycine betaine/L‑proline transporter ProP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

GCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGT  >  NZ_CP009273/4321672‑4321915                                                                                                                                    |                                                                                                                 gcggcgTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGaa                                                                                                           >  1:279441/1‑139 (MQ=255) gcggcgTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGaa                                                                                                           >  2:3801/1‑139 (MQ=255) gcggcgTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGaa                                                                                                           >  1:780130/1‑139 (MQ=255) gcggcgTCAGATATACAGGAAGCGAAGGAAATCCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGaa                                                                                                           >  1:741477/1‑139 (MQ=255)                   gAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGaaa                                                                                         >  1:699707/1‑139 (MQ=255)                     aGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACg                                                                                       >  2:402737/1‑139 (MQ=255)                                     gtcgAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGAc                                                                       <  2:475621/139‑1 (MQ=255)                                      tcgAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACg                                                                      <  2:543804/139‑1 (MQ=255)                                             ttACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCt                                                                >  2:719584/1‑138 (MQ=255)                                               aCGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTAt                                                             >  1:823748/1‑139 (MQ=255)                                                cGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTAt                                                             <  2:741477/138‑1 (MQ=255)                                                  ataataTCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAg                                                          <  2:823748/139‑1 (MQ=255)                                                      tatCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGAc                                                                       <  1:32290/122‑1 (MQ=255)                                                      tatCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGAc                                                                       >  2:32290/1‑122 (MQ=255)                                                        tCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACg                                                                      >  1:26835/1‑121 (MQ=255)                                                        tCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACg                                                                      <  2:26835/121‑1 (MQ=255)                                                        tCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTAt                                                             <  2:40316/130‑1 (MQ=255)                                                        tCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTAt                                                             >  1:40316/1‑130 (MQ=255)                                                        tCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAAt                                                    >  1:360891/1‑139 (MQ=255)                                                          gAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg                                                  <  2:780130/139‑1 (MQ=255)                                                          gAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg                                                  <  1:3801/139‑1 (MQ=255)                                                          gAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg                                                  <  2:279441/139‑1 (MQ=255)                                                           aGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg                                                  >  2:81528/1‑138 (MQ=255)                                                             cAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAAc                                               <  1:81528/139‑1 (MQ=255)                                                                              tGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACtt                                <  1:163136/137‑1 (MQ=255)                                                                              tGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACtt                                >  2:163136/1‑137 (MQ=255)                                                                                  cACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAAtctc                          >  1:382029/1‑139 (MQ=255)                                                                                   aCGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATctct                         <  2:234490/139‑1 (MQ=255)                                                                                    cGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATctct                         >  2:678721/1‑138 (MQ=255)                                                                                     gagaTTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGa                       <  1:333451/139‑1 (MQ=255)                                                                                     gagaTTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGa                       >  1:654989/1‑139 (MQ=255)                                                                                          tGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGAc                                                                       >  2:320796/1‑86 (MQ=255)                                                                                          tGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGAc                                                                       <  1:320796/86‑1 (MQ=255)                                                                                          tGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGAcgcg                   >  1:564951/1‑138 (MQ=255)                                                                                            ccGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCa                 <  1:402737/138‑1 (MQ=255)                                                                                                tttGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATActc            <  1:848845/139‑1 (MQ=255)                                                                                                        cGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAg    <  2:583041/139‑1 (MQ=255)                                                                                                          aaaCGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGt  <  2:210849/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                 GCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGT  >  NZ_CP009273/4321672‑4321915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑