| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,350,177 | A→G | intergenic (‑329/+36) | cadB ← / ← cadC | cadaverine/lysine antiporter/lysine decarboxylation/transport transcriptional activator CadC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,350,177 | 0 | A | G | 97.6% | 150.9 / ‑3.7 | 41 | intergenic (‑329/+36) | cadB/cadC | cadaverine/lysine antiporter/lysine decarboxylation/transport transcriptional activator CadC |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (20/20); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TAAACATTTAATGTTTACAATGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGG > NZ_CP009273/4350054‑4350297 | tAAACATTTAATGTTTACAATGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGc < 1:784119/139‑1 (MQ=255) aaCATTTAATGTTTACAATGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCa < 1:33490/138‑1 (MQ=255) aTTTAATGTTTACAATGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATaaa < 2:697620/139‑1 (MQ=255) aaTGTTTACAATGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTAAACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCaa > 2:550564/1‑139 (MQ=255) tACAATGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCg > 2:571776/1‑137 (MQ=255) tGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTAc > 2:856071/1‑139 (MQ=255) tGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTAtt > 1:268982/1‑139 (MQ=255) gTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTg < 2:645356/139‑1 (MQ=255) gACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTg > 2:214628/1‑137 (MQ=255) gTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAAttt > 2:496341/1‑139 (MQ=255) tAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGt > 2:784292/1‑139 (MQ=255) aaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTc < 1:856071/138‑1 (MQ=255) aaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCg < 2:419774/139‑1 (MQ=255) tGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATa < 1:214628/138‑1 (MQ=255) tGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATa < 2:268982/138‑1 (MQ=255) tGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATa < 1:363214/138‑1 (MQ=255) ataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTa < 1:571776/139‑1 (MQ=255) ataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTa < 2:313750/139‑1 (MQ=255) tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTcc < 1:74793/70‑1 (MQ=255) tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTcc > 2:74793/1‑70 (MQ=255) cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGa < 1:299732/98‑1 (MQ=255) cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGa > 2:299732/1‑98 (MQ=255) cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg < 1:198461/139‑1 (MQ=255) cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg < 2:508162/139‑1 (MQ=255) gCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTAc > 2:661088/1‑79 (MQ=255) gCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTAc < 1:661088/79‑1 (MQ=255) aCATATTATACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATAcc < 2:424029/139‑1 (MQ=255) ttatACCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAtttt < 1:582057/139‑1 (MQ=255) tataCCAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAtttt > 2:582057/1‑138 (MQ=255) cAACAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATc > 1:684698/1‑138 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGaa > 1:798435/1‑107 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGaa < 2:798435/107‑1 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt > 2:542563/1‑114 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGt < 1:542563/114‑1 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg > 2:340427/1‑139 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg > 1:810517/1‑139 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg > 1:584553/1‑139 (MQ=255) aCAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg > 1:575076/1‑139 (MQ=255) cAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg > 1:341628/1‑138 (MQ=255) cAGGAACATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg > 1:672289/1‑138 (MQ=255) aCATACAAAAACTCAACAGCAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAgg > 2:240138/1‑139 (MQ=255) | TAAACATTTAATGTTTACAATGGATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGG > NZ_CP009273/4350054‑4350297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |