Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,359,587 G→T R215S (CGT→AGT)  yjeH ← L‑methionine/branched‑chain amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,359,5870GT97.1% 273.4 / ‑1.0 69R215S (CGT→AGT) yjeHL‑methionine/branched‑chain amino acid transporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (28/39);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

TTTTTGGAAGCGATGCTGCCGCCGCCATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAG  >  NZ_CP009273/4359458‑4359721
                                                                                                                                 |                                                                                                                                      
tttttGGAAGCGATGCTGCCGCCGCCATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGAtt                                                                                                                               >  1:808185/1‑139 (MQ=255)
  tttGGAAGCGATGCTGCCGCCGCCATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGct                                                                                                                                      >  1:626463/1‑130 (MQ=255)
  tttGGAAGCGATGCTGCCGCCGCCATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGct                                                                                                                                      <  2:626463/130‑1 (MQ=255)
        aGCGATGCTGCCGCCGCCATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAAtt                                                                                                                       <  1:800617/139‑1 (MQ=255)
                  ccgccgccATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCg                                                                                                               <  1:59137/137‑1 (MQ=255)
                       gccATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATggg                                                                                                        >  2:674494/1‑139 (MQ=255)
                       gccATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATggg                                                                                                        <  1:522868/139‑1 (MQ=255)
                           ttttttCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCaaa                                                                                                    <  2:23608/139‑1 (MQ=255)
                           ttttttCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCaaa                                                                                                    <  2:486868/139‑1 (MQ=255)
                           ttttttCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCaaa                                                                                                    <  2:57303/139‑1 (MQ=255)
                           ttttttCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCaaa                                                                                                    <  2:808185/139‑1 (MQ=255)
                                         gCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGa                                                                                      <  2:589608/139‑1 (MQ=255)
                                                 gtgtAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGTCGAGATGGGCAAATGCCTCCAGACCGACAAAacac                                                                              <  2:432057/139‑1 (MQ=255)
                                                    tAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAg                                                                           <  1:4611/139‑1 (MQ=255)
                                                    tAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAg                                                                           <  2:808335/139‑1 (MQ=255)
                                                      aGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGa                                                                          <  2:822613/138‑1 (MQ=255)
                                                      aGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGa                                                                          <  2:90363/138‑1 (MQ=255)
                                                            cTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCAc                                                                   >  2:237069/1‑139 (MQ=255)
                                                            cTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCAc                                                                   >  2:643069/1‑139 (MQ=255)
                                                                 gTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATa                                                              <  2:121972/139‑1 (MQ=255)
                                                                    cAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACg                                                           >  2:391051/1‑139 (MQ=255)
                                                                    cAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACg                                                           <  2:77441/139‑1 (MQ=255)
                                                                       ccccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGa                                                                          >  1:486391/1‑121 (MQ=255)
                                                                       ccccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGa                                                                          <  2:486391/121‑1 (MQ=255)
                                                                       ccccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACgcag                                                        >  1:853197/1‑139 (MQ=255)
                                                                       ccccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATCCCGAGGCGAGATGGGCAAATGCCTCCAGAcc                                                                                        <  1:99884/107‑1 (MQ=255)
                                                                       ccccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATCCCGAGGCGAGATGGGCAAATGCCTCCAGAcc                                                                                        >  2:99884/1‑107 (MQ=255)
                                                                         ccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCgag                                                                                                             <  2:434178/84‑1 (MQ=255)
                                                                         ccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCgag                                                                                                             >  1:434178/1‑84 (MQ=255)
                                                                         ccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACaa                                                                                   <  1:177759/110‑1 (MQ=255)
                                                                         ccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACaa                                                                                   >  2:177759/1‑110 (MQ=255)
                                                                         ccAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACgcagc                                                       >  2:860468/1‑138 (MQ=255)
                                                                            gTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAgg                                                                                                                 <  2:462490/77‑1 (MQ=255)
                                                                            gTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAgg                                                                                                                 >  1:462490/1‑77 (MQ=255)
                                                                            gTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCaaa                                                    >  1:425122/1‑138 (MQ=255)
                                                                                 cTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAAccc                                              >  2:124840/1‑139 (MQ=255)
                                                                                  tAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAacac                                                                              >  2:231979/1‑106 (MQ=255)
                                                                                  tAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAacac                                                                              <  1:231979/106‑1 (MQ=255)
                                                                                    aTCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGt                                           <  2:425122/139‑1 (MQ=255)
                                                                                       cTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAg                                        <  1:674494/139‑1 (MQ=255)
                                                                                         gCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCgaga                                                                                                            >  1:759398/1‑69 (MQ=255)
                                                                                         gCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCgaga                                                                                                            <  2:759398/69‑1 (MQ=255)
                                                                                         gCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGtt                                      >  2:226117/1‑139 (MQ=255)
                                                                                         gCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGtt                                      >  2:379078/1‑139 (MQ=255)
                                                                                          ccAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGtt                                      <  1:643069/138‑1 (MQ=255)
                                                                                          ccAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGt                                       <  1:571026/137‑1 (MQ=255)
                                                                                          ccAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTc                                     >  1:31936/1‑139 (MQ=255)
                                                                                              cagcagACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGa                                   >  2:325023/1‑137 (MQ=255)
                                                                                              cagcagACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGa                                   <  1:325023/137‑1 (MQ=255)
                                                                                                   gACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTAcc                            <  2:411427/139‑1 (MQ=255)
                                                                                                   gACCAATCATCAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTAcc                            <  1:633585/139‑1 (MQ=255)
                                                                                                        atcatcAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCaaa                                                                                                    <  1:60428/62‑1 (MQ=255)
                                                                                                        atcatcAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCaaa                                                                                                    >  2:60428/1‑62 (MQ=255)
                                                                                                        atcatcAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAacac                                                                              <  2:17338/84‑1 (MQ=255)
                                                                                                        atcatcAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAacac                                                                              >  1:17338/1‑84 (MQ=255)
                                                                                                        atcatcAAAGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTg                       >  1:248873/1‑139 (MQ=255)
                                                                                                                aGCACGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAg               <  2:31936/139‑1 (MQ=255)
                                                                                                                   aCGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACaa                                                                                   <  1:835775/68‑1 (MQ=255)
                                                                                                                   aCGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACaa                                                                                   >  2:835775/1‑68 (MQ=255)
                                                                                                                   aCGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAgggg            <  1:379078/139‑1 (MQ=255)
                                                                                                                   aCGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAgggg            <  1:226117/139‑1 (MQ=255)
                                                                                                                    cGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGa                                                                          <  1:590248/76‑1 (MQ=255)
                                                                                                                    cGAGGAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGa                                                                          >  2:590248/1‑76 (MQ=255)
                                                                                                                       ggAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAgggg            >  1:669951/1‑135 (MQ=255)
                                                                                                                       ggAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAgggg            <  2:669951/135‑1 (MQ=255)
                                                                                                                       ggAAAATCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGatat        >  1:362696/1‑139 (MQ=255)
                                                                                                                          aaaTCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCg     <  1:124840/139‑1 (MQ=255)
                                                                                                                           aaTCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGc    <  1:860468/139‑1 (MQ=255)
                                                                                                                             tCACTCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAg  >  1:344537/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                      
TTTTTGGAAGCGATGCTGCCGCCGCCATTTTTTCACCATAGGCGTCGAAGTGTAAGACGACTACCGTACAGCCCCAGTAGACTAATCCTGCCAGCAGCAGACCAATCATCAAAGCACGAGGAAAATCACGCTCTGGATTTTTAAATTCCGAGGCGAGATGGGCAAATGCCTCCAGACCGACAAAACACCAGAACATCACTGATAACGCAGCAAATAACCCGGTAAGTTCGATATTACCAGGTGCCGGAAAGGGGATATTCGCAG  >  NZ_CP009273/4359458‑4359721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑