| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,371,321 | T→C | E272G (GAA→GGA) | frdA ← | fumarate reductase (quinol) flavoprotein subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,371,321 | 0 | T | C | 87.1% | 162.2 / 17.6 | 62 | E272G (GAA→GGA) | frdA | fumarate reductase (quinol) flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base C (29/25); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCAT > NZ_CP009273/4371191‑4371457 | gagaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGccc < 1:70799/139‑1 (MQ=255)gagaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGccc < 1:862168/139‑1 (MQ=255)gagaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGcc < 1:433221/138‑1 (MQ=255) agaTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCa > 1:56464/1‑139 (MQ=255) aTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATg > 1:267979/1‑139 (MQ=255) ccACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTa < 2:3070/139‑1 (MQ=255) aTCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATc > 1:254073/1‑139 (MQ=255) gCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTg < 1:836718/138‑1 (MQ=255) cgcgGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGa < 2:254073/139‑1 (MQ=255) cgcgGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGAt < 1:533472/139‑1 (MQ=255) cgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACgg > 2:723659/1‑139 (MQ=255) tGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACgg > 2:751629/1‑137 (MQ=255) gtgtTGCCTTTACGCCATTCGTGCCAGAAGGCTTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtttt > 1:747249/1‑139 (MQ=255) gtgtTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtttt > 2:821491/1‑139 (MQ=255) gCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgtt < 2:530514/138‑1 (MQ=255) gCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgtt < 1:654155/138‑1 (MQ=255) gCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgtt > 1:702389/1‑138 (MQ=255) cTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAc < 2:747249/139‑1 (MQ=255) tACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAcc < 2:702389/137‑1 (MQ=255) aCGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAg > 1:726070/1‑138 (MQ=255) ttCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCg > 1:499969/1‑139 (MQ=255) ttCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCg > 2:809850/1‑139 (MQ=255) ttCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCg > 2:424057/1‑139 (MQ=255) cTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCaa > 2:317116/1‑139 (MQ=255) gaCTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGCCCAGAATACCGCCTTCACCGCGGCAACCTTCg < 1:751629/139‑1 (MQ=255) gaCTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCg < 1:403833/139‑1 (MQ=255) cTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGt > 1:385750/1‑139 (MQ=255) tttGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgtt < 2:546467/102‑1 (MQ=255) tttGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgtt > 1:546467/1‑102 (MQ=255) tttGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGt > 1:232844/1‑138 (MQ=255) tGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgtt > 1:740104/1‑100 (MQ=255) tGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgtt < 2:740104/100‑1 (MQ=255) tGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcat > 2:335719/1‑139 (MQ=255) tGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcat > 1:169559/1‑139 (MQ=255) gTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcat < 2:148979/138‑1 (MQ=255) tCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCtt > 2:374631/1‑73 (MQ=255) tCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCtt < 1:374631/73‑1 (MQ=255) tCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcat > 2:622113/1‑137 (MQ=255) gcgTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAg < 1:821491/138‑1 (MQ=255) gcgTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAgg < 2:385750/139‑1 (MQ=255) tGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAc > 2:669976/1‑96 (MQ=255) tGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAc < 1:669976/96‑1 (MQ=255) gACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATAc < 1:809850/138‑1 (MQ=255) ttCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCt > 2:558461/1‑139 (MQ=255) ttCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCt > 1:683387/1‑139 (MQ=255) atatatTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTgg > 1:393011/1‑137 (MQ=255) atatatTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTgg > 1:607408/1‑137 (MQ=255) atatatTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCa < 2:56464/139‑1 (MQ=255) tatatTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcat < 1:17529/118‑1 (MQ=255) tatatTTGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcat > 2:17529/1‑118 (MQ=255) ttGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAc < 2:352257/77‑1 (MQ=255) ttGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAc > 1:352257/1‑77 (MQ=255) ttGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg > 1:431496/1‑139 (MQ=255) ttGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg > 2:858167/1‑139 (MQ=255) tGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg < 2:169559/138‑1 (MQ=255) tGTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCgg < 1:6705/138‑1 (MQ=255) gTTTTTCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGtt > 2:355596/1‑139 (MQ=255) tttCGGCT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTggg > 1:77118/1‑139 (MQ=255) tCGGCT‑CGCCCGGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGt < 2:77232/138‑1 (MQ=255) cT‑CGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATAc < 1:622113/139‑1 (MQ=255) ccAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAAc < 2:77118/139‑1 (MQ=255) ggAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAAtt < 1:558461/139‑1 (MQ=255) tttCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCAt > 2:217202/1‑139 (MQ=255) | GAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCT‑CGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCAT > NZ_CP009273/4371191‑4371457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |