Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,374,268	T→C	S205P (TCC→CCC)	yjeM →	glutamate/gamma‑aminobutyrate family transporter YjeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,374,268	0	T	C	100.0%	153.0 / NA	42	S205P (TCC→CCC)	yjeM	glutamate/gamma‑aminobutyrate family transporter YjeM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/15);  total (27/15)

GTGGGCGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGTCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCGCT  >  NZ_CP009273/4374139‑4374399                                                                                                                                  |                                                                                                                                    gTGGGCGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTgg                                                                                                                            >  2:714035/1‑139 (MQ=255)      cGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATg                                                                                                                       >  1:718617/1‑139 (MQ=255)         tATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTa                                                                                                                    >  2:237442/1‑139 (MQ=255)         tATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTa                                                                                                                    >  2:383926/1‑139 (MQ=255)           ttGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTAt                                                                                                                   <  2:281102/138‑1 (MQ=255)           ttGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTAt                                                                                                                   <  1:45215/138‑1 (MQ=255)                   aTGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTgg                                                                                                          >  2:157398/1‑139 (MQ=255)                              tttAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCAtt                                                                                               <  1:819607/139‑1 (MQ=255)                                        cTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATg                                                                                     >  1:577956/1‑139 (MQ=255)                                           ttAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                                    <  2:591263/137‑1 (MQ=255)                                           ttAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCg                                                                                  >  2:480876/1‑139 (MQ=255)                                                 aGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCAttt                                                                                              >  2:125085/1‑121 (MQ=255)                                                 aGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCAttt                                                                                              <  1:125085/121‑1 (MQ=255)                                                       aCTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCgg                                                                      >  2:329962/1‑139 (MQ=255)                                                             ttGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCTAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGGGGTATTTGCCATTTTTGCCTATGGCGGAGTTGAAGCGGTtggtg                                                                >  1:428093/1‑139 (MQ=255)                                                                ttattaAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGtggtc                                                             >  1:348810/1‑139 (MQ=255)                                                                  attaAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTgg                                                                                                                            <  2:269708/74‑1 (MQ=255)                                                                  attaAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTgg                                                                                                                            >  1:269708/1‑74 (MQ=255)                                                                     aaaTGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGtggtctggtc                                                        >  1:684597/1‑139 (MQ=255)                                                                          gtggGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATa                                                    >  1:759271/1‑138 (MQ=255)                                                                             ggCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAAc                                                >  2:320475/1‑139 (MQ=255)                                                                               cATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACg                                               >  1:289128/1‑138 (MQ=255)                                                                                aTTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGa                                             <  1:237442/139‑1 (MQ=255)                                                                                aTTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGa                                             <  1:383926/139‑1 (MQ=255)                                                                                          ggATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGa                                    >  2:630476/1‑138 (MQ=255)                                                                                                     tCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGc                        >  1:585754/1‑139 (MQ=255)                                                                                                     tCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGc                        >  2:384492/1‑139 (MQ=255)                                                                                                     tCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGc                        >  1:780796/1‑139 (MQ=255)                                                                                                       cTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGcc                       >  2:307392/1‑138 (MQ=255)                                                                                                       cTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGcc                       >  2:430991/1‑138 (MQ=255)                                                                                                        ttGCATCACCGAACCCGGGTTATCAGCCCGGTCTgg                                                                                                                            >  1:75959/1‑36 (MQ=255)                                                                                                        ttGCATCACCGAACCCGGGTTATCAGCCCGGTCTgg                                                                                                                            <  2:75959/36‑1 (MQ=255)                                                                                                          gCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCaa                     <  2:780796/137‑1 (MQ=255)                                                                                                                ccGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTg             >  1:46396/1‑139 (MQ=255)                                                                                                                 cGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGt            >  1:508155/1‑139 (MQ=255)                                                                                                                       cGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGc      <  2:584616/139‑1 (MQ=255)                                                                                                                         ggTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCg    <  1:72877/139‑1 (MQ=255)                                                                                                                         ggTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCg    <  2:577956/139‑1 (MQ=255)                                                                                                                          gTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCGc   <  2:866657/139‑1 (MQ=255)                                                                                                                          gTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCGc   >  2:755621/1‑139 (MQ=255)                                                                                                                          gTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCGc   >  2:547066/1‑139 (MQ=255)                                                                                                                           ttATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCGCt  <  1:480876/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    GTGGGCGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGTCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCAAAGGTATTGTTTTTGCCGCT  >  NZ_CP009273/4374139‑4374399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑