Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,395,911	T→C	intergenic (+109/‑96)	purA → / → nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,395,911	0	T	C	100.0%	150.6 / NA	42	intergenic (+109/‑96)	purA/nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/16);  total (26/16)

ATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCG  >  NZ_CP009273/4395776‑4396045                                                                                                                                        |                                                                                                                                       aTTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTtccct                                                                                                                                     <  2:791938/139‑1 (MQ=255)     tGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACCTTGCTGTGTCGGGTAAGCCCTTACGCTATCCGACCCCGTGTTTAATCCTCGCTTTttcccttccc                                                                                                                                 >  2:233422/1‑139 (MQ=255)     tGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTttcccttccc                                                                                                                                 >  2:389310/1‑139 (MQ=255)        gcgACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTGTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGa                                                                                                                              <  2:552297/139‑1 (MQ=255)            cccGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACt                                                                                                                           >  1:248432/1‑138 (MQ=255)                   gACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGaaataa                                                                                                                    <  2:425394/138‑1 (MQ=255)                            ttCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGaca                                                                                                          <  1:233422/139‑1 (MQ=255)                                 gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCt                                                                                                     <  2:142445/139‑1 (MQ=255)                                  tACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCtt                                                                                                    <  2:748720/139‑1 (MQ=255)                                      ccTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                                                                <  1:852360/139‑1 (MQ=255)                                          gCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCt                                                                                              <  1:86632/137‑1 (MQ=255)                                                   ttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcat                                                                                    <  1:755200/138‑1 (MQ=255)                                                   ttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcat                                                                                    <  2:48384/138‑1 (MQ=255)                                                        tgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcat                                                                                    <  2:139394/133‑1 (MQ=255)                                                        tgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcat                                                                                    >  1:139394/1‑133 (MQ=255)                                                                        tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTccc                                                              >  2:2327/1‑139 (MQ=255)                                                                             cATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                         <  1:521100/139‑1 (MQ=255)                                                                              aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTc                                                        >  1:669453/1‑139 (MQ=255)                                                                                tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGttt                                                                                         <  1:643228/104‑1 (MQ=255)                                                                                tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                      >  2:578068/1‑139 (MQ=255)                                                                                tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                      >  1:561112/1‑139 (MQ=255)                                                                                tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                      >  1:500194/1‑139 (MQ=255)                                                                                tGCTGTGTCGGGTAAGCCATTACGCTATCCGACAAAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGttt                                                                                         >  2:643228/1‑104 (MQ=255)                                                                                  cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtt                                                    >  1:322804/1‑139 (MQ=255)                                                                                         gggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                              <  1:86740/138‑1 (MQ=255)                                                                                          ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGtt                                            >  1:571048/1‑139 (MQ=255)                                                                                                    tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGt                                  >  1:807726/1‑139 (MQ=255)                                                                                                    tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGt                                  >  2:152108/1‑139 (MQ=255)                                                                                                           tCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                                                                >  1:775487/1‑70 (MQ=255)                                                                                                           tCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                                                                <  2:775487/70‑1 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAg                                                                                                               <  2:462291/45‑1 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAg                                                                                                               >  1:462291/1‑45 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  1:792438/1‑139 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  2:592108/1‑139 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  2:776057/1‑139 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  2:594031/1‑139 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  2:156447/1‑139 (MQ=255)                                                                                                                     tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  1:554821/1‑139 (MQ=255)                                                                                                                             ccTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggat          >  1:744871/1‑138 (MQ=255)                                                                                                                               tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  2:818360/1‑139 (MQ=255)                                                                                                                               tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  2:582688/1‑139 (MQ=255)                                                                                                                               tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  1:358734/1‑139 (MQ=255)                                                                                                                               tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  1:289881/1‑139 (MQ=255)                                                                                                                               tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  1:157326/1‑139 (MQ=255)                                                                                                                                  cTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTg    <  1:2327/139‑1 (MQ=255)                                                                                                                                     tttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg  <  2:571048/138‑1 (MQ=255)                                                                                                                                     tttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg  <  2:157326/138‑1 (MQ=255)                                                                                                                                     tttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg  <  2:669453/138‑1 (MQ=255)                                                                                                                                     tttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg  <  1:292980/138‑1 (MQ=255)                                                                                                                                     tttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg  <  1:272190/138‑1 (MQ=255)                                                                                                                                     tttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg  <  2:792438/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCG  >  NZ_CP009273/4395776‑4396045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑