Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,428,964	T→C	E39G (GAG→GGG)	ytfJ ←	YtfJ family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,428,964	0	T	C	100.0%	210.8 / NA	60	E39G (GAG→GGG)	ytfJ	YtfJ family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/32);  total (28/32)

ATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGATA  >  NZ_CP009273/4428829‑4429099                                                                                                                                        |                                                                                                                                        aTCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGc                                                                                                                                      >  1:791328/1‑139 (MQ=255)        cAATCATCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCGCTTTTCCCACTAACTGGGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCCCCAACCCGCCCCGa                                                                                                                                 <  2:462623/137‑1 (MQ=255)          aTCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCgg                                                                                                                             <  2:678957/139‑1 (MQ=255)                    cgTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAAt                                                                                                                   >  1:142530/1‑139 (MQ=255)                                     gagGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                                                                  <  1:72812/139‑1 (MQ=255)                                        gTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCgg                                                                                               >  2:109929/1‑139 (MQ=255)                                          gCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGtt                                                                                             >  2:569546/1‑139 (MQ=255)                                           cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATcggcgg                                                                                                             <  1:236798/122‑1 (MQ=255)                                           cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATcggcgg                                                                                                             >  2:236798/1‑122 (MQ=255)                                           cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGttt                                                                                            >  1:47620/1‑139 (MQ=255)                                           cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGtt                                                                                             <  1:657258/138‑1 (MQ=255)                                               cAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGaa                                                                                        <  2:396323/139‑1 (MQ=255)                                                aGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAAc                                                                                       >  1:143522/1‑139 (MQ=255)                                                 gCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACt                                                                                      >  2:357670/1‑139 (MQ=255)                                                  cAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTg                                                                                     <  2:299341/139‑1 (MQ=255)                                                    atatGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAt                                                                                   <  2:142530/139‑1 (MQ=255)                                                         ttGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCg                                                                              <  1:528019/139‑1 (MQ=255)                                                          tGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCg                                                                              >  1:600969/1‑138 (MQ=255)                                                            cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCgg                                                                             >  1:283125/1‑137 (MQ=255)                                                               tACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGc                                                                         >  1:181704/1‑138 (MQ=255)                                                               tACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGc                                                                         >  1:559783/1‑138 (MQ=255)                                                                 cTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcat                                                                      >  1:651187/1‑139 (MQ=255)                                                                 cTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGCCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcat                                                                      >  1:155942/1‑139 (MQ=255)                                                                 cTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATC‑TTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                                                                     >  2:2571/1‑139 (MQ=255)                                                                    gCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatcat                                                                   >  2:255604/1‑139 (MQ=255)                                                                    gCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatca                                                                    <  1:594565/138‑1 (MQ=255)                                                                    gCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatc                                                                     <  2:792621/137‑1 (MQ=255)                                                                       cTTTTCCCACTACCTGCGCGCGTTTCCAGTTTTGGTAGCTAACTGGGTCTTTACCAACCACC‑ACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGc                                                               <  2:275871/139‑1 (MQ=255)                                                                         tttCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGc                                                               >  1:439095/1‑138 (MQ=255)                                                                          ttCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGc                                                               <  2:159396/137‑1 (MQ=255)                                                                            cccACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAAca                                                           <  2:600969/139‑1 (MQ=255)                                                                             ccACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAAca                                                           <  2:598299/138‑1 (MQ=255)                                                                                  aaCTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGAt                                                                                                                                <  2:220360/64‑1 (MQ=255)                                                                                  aaCTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGAt                                                                                                                                >  1:220360/1‑64 (MQ=255)                                                                                    cTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAgcaggcag                                                   >  2:67258/1‑139 (MQ=255)                                                                                      gcgcgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGt                                                 <  2:141865/139‑1 (MQ=255)                                                                                      gcgcgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGt                                                 <  2:181704/139‑1 (MQ=255)                                                                                         cgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAg                                              <  2:817108/139‑1 (MQ=255)                                                                                                   ggTTTTGTATCTAATCTGATTTTGACCACCCCCCAACCCGCCCCGATCGGTAATGCCAACCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTTCCAGAATc                                    <  2:143522/139‑1 (MQ=255)                                                                                                        tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               >  2:25327/1‑139 (MQ=255)                                                                                                        tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               >  2:394573/1‑139 (MQ=255)                                                                                                        tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               >  2:755708/1‑139 (MQ=255)                                                                                                        tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               >  2:530509/1‑139 (MQ=255)                                                                                                         gTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               <  1:755708/138‑1 (MQ=255)                                                                                                         gTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               <  2:283125/138‑1 (MQ=255)                                                                                                          tAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTa                             <  2:791328/139‑1 (MQ=255)                                                                                                          tAGCTAAACTGATCTTTACCAAGCCCCACCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTa                             <  2:352066/139‑1 (MQ=255)                                                                                                                aaCTGGTCTTTATCAACCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCCCTGACCGGTTTCGAACTGATGTTCGGATGCCGTCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTc                        <  2:682982/138‑1 (MQ=255)                                                                                                                    gATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATc                                    >  2:18910/1‑122 (MQ=255)                                                                                                                    gATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATc                                    <  1:18910/122‑1 (MQ=255)                                                                                                                     aTCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAAt                                     >  2:200349/1‑120 (MQ=255)                                                                                                                     aTCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAAt                                     <  1:200349/120‑1 (MQ=255)                                                                                                                          tATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGttt               <  2:47620/137‑1 (MQ=255)                                                                                                                               aGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCcttc          <  2:315617/137‑1 (MQ=255)                                                                                                                               aGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCcttc          >  1:315617/1‑137 (MQ=255)                                                                                                                                 caccacCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTa                             <  1:93201/116‑1 (MQ=255)                                                                                                                                 cACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTa                             >  2:93201/1‑116 (MQ=255)                                                                                                                                  aCCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTAt      >  1:621210/1‑138 (MQ=255)                                                                                                                                  aCCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATg     <  2:621210/139‑1 (MQ=255)                                                                                                                                     aaCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGata  <  1:67258/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGATA  >  NZ_CP009273/4428829‑4429099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑