Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,449,957 A→G D75D (GAT→GAC)  nrdG ← anaerobic ribonucleoside‑triphosphate reductase‑activating protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,449,9570AG93.8% 142.0 / 0.8 48D75D (GAT→GAC) nrdGanaerobic ribonucleoside‑triphosphate reductase‑activating protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (21/24);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TCAACAACCTGCATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGG  >  NZ_CP009273/4449820‑4450092
                                                                                                                                         |                                                                                                                                       
tcaaCAACCTGCATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGAt                                                                                                                                        <  1:684272/139‑1 (MQ=255)
  aacaacCTGCATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTcg                                                                                                                                      >  2:168104/1‑139 (MQ=255)
       ccTGCATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCgccgcc                                                                                                                                 >  2:441832/1‑139 (MQ=255)
        cTGCATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGccgccg                                                                                                                                <  1:474026/139‑1 (MQ=255)
           cATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCgg                                                                                                                               <  2:714500/137‑1 (MQ=255)
           cATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGga                                                                                                                              <  1:464013/138‑1 (MQ=255)
                 cgcAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGaa                                                                                                                       <  2:580676/139‑1 (MQ=255)
                 cgcAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGaa                                                                                                                       <  1:409546/139‑1 (MQ=255)
                      cGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATAcc                                                                                                                  >  1:637421/1‑139 (MQ=255)
                               cgtcgAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGtt                                                                                                          <  1:844442/138‑1 (MQ=255)
                                gtcgAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                                                                                         >  2:400126/1‑138 (MQ=255)
                                gtcgAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                                                                                         >  1:481654/1‑138 (MQ=255)
                                gtcgAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                                                                                         >  1:703794/1‑138 (MQ=255)
                                gtcgAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                                                                                         >  1:111502/1‑138 (MQ=255)
                                gtcgAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGttt                                                                                                         >  1:520831/1‑138 (MQ=255)
                                   gAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATa                                                                                                     <  2:111502/139‑1 (MQ=255)
                                          taGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAgtgt                                                                                              >  2:288239/1‑139 (MQ=255)
                                               tGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCAtt                                                                                          >  2:9075/1‑138 (MQ=255)
                                                gTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCAtt                                                                                          >  1:75623/1‑137 (MQ=255)
                                                 tCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAg                                                                                       <  2:828082/139‑1 (MQ=255)
                                                   cacaCCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGAt                                                                                     <  1:627331/139‑1 (MQ=255)
                                                   cacaCCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGAt                                                                                     <  1:740882/139‑1 (MQ=255)
                                                        ccAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGtt                                                                                 >  2:812093/1‑138 (MQ=255)
                                                           gATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGAt                                                                                     <  2:552958/131‑1 (MQ=255)
                                                           gATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGAt                                                                                     >  1:552958/1‑131 (MQ=255)
                                                           gATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAAt                                                                              >  1:496146/1‑138 (MQ=255)
                                                             tGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGAt                                                                           <  1:580719/139‑1 (MQ=255)
                                                               tCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATc                                                                          <  1:734617/138‑1 (MQ=255)
                                                               tCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATc                                                                          <  1:345176/138‑1 (MQ=255)
                                                               tCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATc                                                                          <  1:588059/138‑1 (MQ=255)
                                                                      ccGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATAc                                                                                                    >  1:357728/1‑105 (MQ=255)
                                                                      ccGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATAc                                                                                                    <  2:357728/105‑1 (MQ=255)
                                                                        ggACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCa                                                                >  1:509662/1‑139 (MQ=255)
                                                                         gACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCAt                                                               >  1:35937/1‑139 (MQ=255)
                                                                         gACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACACTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCa                                                                >  1:367126/1‑138 (MQ=255)
                                                                         gACACTCGGCGCGGATGCGTTGTACCAGTGTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCa                                                                <  2:367126/138‑1 (MQ=255)
                                                                                              gTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGAc                                           <  1:46883/138‑1 (MQ=255)
                                                                                                 ccAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCgg                                        >  1:486479/1‑138 (MQ=255)
                                                                                                     tttCAGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAAtt                                    >  1:572483/1‑138 (MQ=255)
                                                                                                         aGAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTAccc                               >  2:604490/1‑139 (MQ=255)
                                                                                                          gAATATCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCg                              <  2:75623/139‑1 (MQ=255)
                                                                                                            atatCCGGCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCTTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGcc                            <  1:604490/139‑1 (MQ=255)
                                                                                                                  ggCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGc                      <  1:168104/139‑1 (MQ=255)
                                                                                                                  ggCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGAAAATCCCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGc                      <  2:338297/139‑1 (MQ=255)
                                                                                                                   gCACGTTTTGCGGATGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCt                     <  2:520831/139‑1 (MQ=255)
                                                                                                                                 tGCAGCGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAAc       >  2:311150/1‑139 (MQ=255)
                                                                                                                                      cGGGTCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCgggg  <  1:400126/139‑1 (MQ=255)
                                                                                                                                      cGGATCGCCGCCGGAGAGGGAAATCCCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCgggg  <  1:180602/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TCAACAACCTGCATTTGCGCAGCGTTGAGTTCGTCGAGTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGG  >  NZ_CP009273/4449820‑4450092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑