Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,466,373 T→A S374T (TCC→ACC)  BW25113_RS22080 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,466,3730TA76.9% 55.4 / 12.8 26S374T (TCC→ACC) BW25113_RS22080hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/1);  new base A (8/12);  total (13/13)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAA  >  NZ_CP009273/4466238‑4466507
                                                                                                                                       |                                                                                                                                      
gATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTTTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGATTAACCTTACCCCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATcc                                                                                                                                      <  1:575597/138‑1 (MQ=255)
 aTAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAAccc                                                                                                                                     <  2:754455/138‑1 (MQ=255)
  tAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCa                                                                                                                                   <  2:829867/139‑1 (MQ=255)
                gTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAatgatg                                                                                                                      >  1:82037/1‑138 (MQ=255)
                       tGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAatga                                                                                                                        >  2:208951/1‑129 (MQ=255)
                       tGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAatga                                                                                                                        <  1:208951/129‑1 (MQ=255)
                                              aCCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAg                                                                                       >  1:266377/1‑139 (MQ=255)
                                                       atatTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCa                                                                                 <  2:485223/136‑1 (MQ=255)
                                                             ttATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTtga                                                                        >  1:404970/1‑139 (MQ=255)
                                                             ttATTGATTCCTTAGATGACGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTtga                                                                        >  2:416156/1‑139 (MQ=255)
                                                               aTTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTGAAAAACCCCATATATTAATGATGTAAGAATAc                                                                                                             <  1:753488/100‑1 (MQ=255)
                                                               aTTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATAc                                                                                                             >  2:753488/1‑100 (MQ=255)
                                                               aTTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTtgat                                                                       >  1:467332/1‑138 (MQ=255)
                                                                     tCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACt                                                                 >  1:403888/1‑138 (MQ=255)
                                                                     tCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACt                                                                 >  2:813463/1‑138 (MQ=255)
                                                                           gatgatGCAAAGGTTAACCTTACACCGTTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGa                                                          <  2:24084/139‑1 (MQ=255)
                                                                                                acacCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGta                                      <  1:813463/138‑1 (MQ=255)
                                                                                                      gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAAt                                >  2:44730/1‑138 (MQ=255)
                                                                                                      gTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATAtt                                                                                                                             <  2:6418/45‑1 (MQ=255)
                                                                                                      gTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATAtt                                                                                                                             >  1:6418/1‑45 (MQ=255)
                                                                                                      gTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATaa                                                       >  2:565916/1‑115 (MQ=255)
                                                                                                      gTCATCGATTCGATTCTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATaa                                                       <  1:565916/115‑1 (MQ=255)
                                                                                                                 gattcTGGAGACTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCt                     <  2:82037/138‑1 (MQ=255)
                                                                                                                          gaCTTTTTCAAAAACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGAt           <  1:770279/139‑1 (MQ=255)
                                                                                                                             tttttCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAg        >  2:169451/1‑139 (MQ=255)
                                                                                                                                   aaaaACCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTaaa  <  2:467332/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAA  >  NZ_CP009273/4466238‑4466507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: