Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,556,234 T→C E277G (GAA→GGA)  yjiN ← DUF445 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,556,2340TC95.9% 143.9 / ‑0.4 49E277G (GAA→GGA) yjiNDUF445 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (19/28);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

CACGAGAATCTTCACTGTTGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTTCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAACCAACTCCGCGCTATG  >  NZ_CP009273/4556103‑4556367
                                                                                                                                   |                                                                                                                                     
cacGAGAATCTTCACTGTTGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATc                                                                                                                                >  1:144917/1‑139 (MQ=255)
cacGAGAATCTTCACTGTTGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATc                                                                                                                                >  2:162393/1‑139 (MQ=255)
            cACTGTTGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCa                                                                                                                        <  1:820272/135‑1 (MQ=255)
                 ttGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGa                                                                                                               >  2:178499/1‑139 (MQ=255)
                  tGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCa                                                                                                                        <  2:350000/129‑1 (MQ=255)
                  tGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCa                                                                                                                        >  1:350000/1‑129 (MQ=255)
                   gATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGAt                                                                                                              <  1:362845/138‑1 (MQ=255)
                    atatCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATc                                                                                                             <  2:324702/138‑1 (MQ=255)
                       tCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGAt                                                                                                              >  2:478677/1‑134 (MQ=255)
                       tCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGAt                                                                                                              <  1:478677/134‑1 (MQ=255)
                          aCTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGa                                                                                                      <  2:130152/139‑1 (MQ=255)
                                      tCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGAt                                                                                          >  1:203191/1‑139 (MQ=255)
                                        ccGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATc                                                                                         <  1:784197/138‑1 (MQ=255)
                                         cGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCaa                                                                                       <  1:162393/139‑1 (MQ=255)
                                                 cccccACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATgg                                                                               <  2:780963/139‑1 (MQ=255)
                                                 cccccACAATTCACGGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCGTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATgg                                                                               <  1:307515/139‑1 (MQ=255)
                                                          ttCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGgc                                                                                               >  2:653834/1‑114 (MQ=255)
                                                          ttCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGgc                                                                                               <  1:653834/114‑1 (MQ=255)
                                                            cACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGAt                                                                     <  1:120655/138‑1 (MQ=255)
                                                             aCTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCa                                                                                                                        <  2:491173/86‑1 (MQ=255)
                                                             aCTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCa                                                                                                                        >  1:491173/1‑86 (MQ=255)
                                                                 agaTAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATgcgc                                                                 <  1:719408/137‑1 (MQ=255)
                                                                 agaTAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCa                                                                <  1:83672/138‑1 (MQ=255)
                                                                 agaTAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCa                                                                <  2:203191/138‑1 (MQ=255)
                                                                    tAGCGATTTAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGAt                                                            <  1:670963/139‑1 (MQ=255)
                                                                    tAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGAt                                                            <  1:467301/139‑1 (MQ=255)
                                                                       cGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCCCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATcgc                                                         >  2:209131/1‑139 (MQ=255)
                                                                         aTTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGa                                                       >  1:504832/1‑139 (MQ=255)
                                                                         aTTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCA‑TTCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGAc                                                      >  2:805559/1‑139 (MQ=255)
                                                                          ttAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGAc                                                      <  2:504832/139‑1 (MQ=255)
                                                                           tAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCa                                                                                                                        >  1:716102/1‑72 (MQ=255)
                                                                           tAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCa                                                                                                                        <  2:716102/72‑1 (MQ=255)
                                                                            aaaaGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACt                                                     >  2:433113/1‑138 (MQ=255)
                                                                                    cATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGgcg                                                                                              >  1:259783/1‑89 (MQ=255)
                                                                                    cATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGgcg                                                                                              <  2:259783/89‑1 (MQ=255)
                                                                                    cATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATatcat                                             >  1:200942/1‑138 (MQ=255)
                                                                                     aTCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATatcatc                                            <  1:433113/138‑1 (MQ=255)
                                                                                          cttTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCaa                                          <  1:714965/135‑1 (MQ=255)
                                                                                                     cTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATc                                                                                                             >  2:474034/1‑57 (MQ=255)
                                                                                                     cTTTTTACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATc                                                                                                             <  1:474034/57‑1 (MQ=255)
                                                                                                       ttttACGGCATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACcgcg                         >  1:492138/1‑139 (MQ=255)
                                                                                                              gCATCGGCTCGCGCTGCCA‑TTCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAAc                 <  1:805559/139‑1 (MQ=255)
                                                                                                               cATCGGCTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAAc                 >  1:503546/1‑139 (MQ=255)
                                                                                                                     cTCGCGCTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAACCAACTc           <  1:714764/139‑1 (MQ=255)
                                                                                                                         cgcTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAACCAACTCcgcg       <  1:754697/139‑1 (MQ=255)
                                                                                                                          gcTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGa                                                       <  1:533083/90‑1 (MQ=255)
                                                                                                                          gcTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGa                                                       >  2:533083/1‑90 (MQ=255)
                                                                                                                          gcTGCCATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAACCAACTCCgcgc      <  1:178499/139‑1 (MQ=255)
                                                                                                                              ccATTCCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAACCAACTCCGCGCTATg  >  2:786691/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CACGAGAATCTTCACTGTTGATATCCACTTTCAGCCATTCCCGTAAATCCCCCCACAATTCACTGAGATAGCGATTAAAAGCTTCATCTTCTTTCAGATAGCTTTTTACGGCATCGGCTCGCGCTGCCATTTCCGGATCGTTTTTCAGCTTGTCGATCAGGGCGAAGGTGGCGCGATCAAACGCATGGCGGATCTGATGCGCACGATCGCGACTAATATCATCAAGCAAAGAATTCACCGCGTCAGAAACCAACTCCGCGCTATG  >  NZ_CP009273/4556103‑4556367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑