Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,568,721	A→G	I145T (ATC→ACC)	mcrB ←	5‑methylcytosine‑specific restriction endonuclease subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,568,721	0	A	G	100.0%	136.2 / NA	36	I145T (ATC→ACC)	mcrB	5‑methylcytosine‑specific restriction endonuclease subunit McrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/15);  total (21/15)

TTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGTT  >  NZ_CP009273/4568588‑4568855                                                                                                                                      |                                                                                                                                       tttGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                   <  1:710840/139‑1 (MQ=255) tttGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                   <  2:259893/139‑1 (MQ=255)            tctcTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtgtt                                                                                                                                 <  1:234424/130‑1 (MQ=255)            tctcTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtgtt                                                                                                                                 >  2:234424/1‑130 (MQ=255)            tctcTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCa                                                                                                                         >  2:789674/1‑138 (MQ=255)                 aTTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAgg                                                                                                                   >  2:323849/1‑139 (MQ=255)                       gTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATc                                                                                                             >  1:823648/1‑139 (MQ=255)                       gTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATc                                                                                                             >  1:851369/1‑139 (MQ=255)                       gTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATc                                                                                                             >  2:293222/1‑139 (MQ=255)                            aGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGgt                                                                                                         <  2:38545/138‑1 (MQ=255)                                  aaaCAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATc                                                                                                   >  2:185854/1‑138 (MQ=255)                                   aaCAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCa                                                                                                  <  1:323849/138‑1 (MQ=255)                                        aTCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                   <  2:476685/100‑1 (MQ=255)                                        aTCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                   >  1:476685/1‑100 (MQ=255)                                              tAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCtgt                                                                                         >  2:712940/1‑136 (MQ=255)                                                          cAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGaa                                                                          <  1:293222/139‑1 (MQ=255)                                                          cAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGa                                                                           <  2:808064/138‑1 (MQ=255)                                                                 tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                   <  2:235109/75‑1 (MQ=255)                                                                 tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                   >  1:235109/1‑75 (MQ=255)                                                                 tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCg                                                                    >  1:646339/1‑138 (MQ=255)                                                                           tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTg                                                         >  1:121892/1‑139 (MQ=255)                                                                           tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTg                                                         >  2:375946/1‑139 (MQ=255)                                                                                           aaTAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg                                                                      >  1:359087/1‑110 (MQ=255)                                                                                           aaTAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg                                                                      <  2:359087/110‑1 (MQ=255)                                                                                                cacTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGAtata                                     >  1:658803/1‑138 (MQ=255)                                                                                                cacTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGAtata                                     >  1:360529/1‑138 (MQ=255)                                                                                                     ttGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTaa                                                                                                     >  1:439536/1‑69 (MQ=255)                                                                                                     ttGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTaa                                                                                                     <  2:439536/69‑1 (MQ=255)                                                                                                       gCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCGTAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg                                                                      <  1:309494/98‑1 (MQ=255)                                                                                                       gCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCGTAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg                                                                      >  2:309494/1‑98 (MQ=255)                                                                                                            aaTTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGc                        >  1:857944/1‑139 (MQ=255)                                                                                                                             taGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCTTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGa       <  1:279092/139‑1 (MQ=255)                                                                                                                             taGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGa       <  1:375946/139‑1 (MQ=255)                                                                                                                                  gTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGtt  >  2:715834/1‑139 (MQ=255)                                                                                                                                   ttGGTTATGTTGTCCAGCATAGAGGCAAATCGGgtgt                                                                                                       >  2:188574/1‑37 (MQ=255)                                                                                                                                   ttGGTTATGTTGTCCAGCATAGAGGCAAATCGGgtgt                                                                                                       <  1:188574/37‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGTT  >  NZ_CP009273/4568588‑4568855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑