Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	487,218	A→C	K117N (AAA→AAC)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	487,218	0	A	C	100.0%	121.6 / NA	35	K117N (AAA→AAC)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/18);  total (17/18)

CGGTAGCTCTGGGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGA  >  NZ_CP009273/487085‑487346                                                                                                                                      |                                                                                                                                 cGGTAGCTCTGGGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCggg                                                                                                                             <  2:284487/139‑1 (MQ=255)                tgggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTAAGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCtggt                                                                                                              >  2:543675/1‑139 (MQ=255)                 gggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCtggtg                                                                                                             >  1:17481/1‑139 (MQ=255)                      ttGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGga                                                                                                        <  1:425002/139‑1 (MQ=255)                      ttGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTATGGTGGTGga                                                                                                        >  2:510255/1‑139 (MQ=255)                        ggCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGgac                                                                                                       >  2:612728/1‑138 (MQ=255)                                   ggTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGc                                                                                            >  1:440470/1‑138 (MQ=255)                                       cGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcg                                                                                       >  1:543774/1‑139 (MQ=255)                                         tAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTG‑‑ATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                   <  1:523993/139‑1 (MQ=255)                                           aaCCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                   <  2:650752/139‑1 (MQ=255)                                                ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACa                                                                                                                        >  1:357515/1‑97 (MQ=255)                                                ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACa                                                                                                                        <  2:357515/97‑1 (MQ=255)                                                ggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTa                                                                              >  2:280556/1‑139 (MQ=255)                                                         ccGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                       <  2:659778/137‑1 (MQ=255)                                                                 aaCCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTaaa                                                             >  2:82166/1‑139 (MQ=255)                                                                               cTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCt                                               <  2:688277/139‑1 (MQ=255)                                                                                   cGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGggt                                           <  1:185337/139‑1 (MQ=255)                                                                                          gAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGaa                                     <  2:320951/138‑1 (MQ=255)                                                                                             tACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACaa                                                                                                                       <  2:375053/53‑1 (MQ=255)                                                                                             tACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACaa                                                                                                                       >  1:375053/1‑53 (MQ=255)                                                                                                ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCt                               >  1:597644/1‑138 (MQ=255)                                                                                                    ccGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGc                          >  1:300841/1‑139 (MQ=255)                                                                                                          aGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                    <  2:17481/139‑1 (MQ=255)                                                                                                          aGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                    <  2:129018/139‑1 (MQ=255)                                                                                                          aGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                    <  2:543774/139‑1 (MQ=255)                                                                                                          aGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                    <  2:836553/139‑1 (MQ=255)                                                                                                          aGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                    <  2:78417/139‑1 (MQ=255)                                                                                                            cTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCa                  <  1:851279/139‑1 (MQ=255)                                                                                                                 gaTCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCAtt                <  1:808741/136‑1 (MQ=255)                                                                                                                 gaTCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCAtt                >  2:808741/1‑136 (MQ=255)                                                                                                                      aCGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGAGGTGGACGATCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCt        >  1:99671/1‑139 (MQ=255)                                                                                                                      aCGTTGATGCCATCAACCCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGGGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCt        >  2:711681/1‑139 (MQ=255)                                                                                                                        gTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGt      <  2:28178/139‑1 (MQ=255)                                                                                                                            aTGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGa  >  1:487344/1‑139 (MQ=255)                                                                                                                            aTGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGa  >  1:661544/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                 CGGTAGCTCTGGGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGA  >  NZ_CP009273/487085‑487346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑