| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 509,698 | A→C | T84P (ACG→CCG) | cueR → | Cu(I)‑responsive transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 509,698 | 0 | A | C | 95.1% | 132.3 / ‑1.2 | 41 | T84P (ACG→CCG) | cueR | Cu(I)‑responsive transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (21/18); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01 | |||||||||||
CACGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGC > NZ_CP009273/509565‑509832 | cacGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTg > 1:294404/1‑139 (MQ=255) agcagcaTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGgag > 2:176804/1‑137 (MQ=255) gcagcaTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAg < 1:300384/139‑1 (MQ=255) ctcAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGCTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCg > 2:776663/1‑138 (MQ=255) tGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAAc > 1:291528/1‑138 (MQ=255) gACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGa > 2:284574/1‑139 (MQ=255) gACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGa > 2:634988/1‑139 (MQ=255) cgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgagg < 1:674641/139‑1 (MQ=255) cgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgagg < 1:79645/139‑1 (MQ=255) cgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgag < 1:630814/138‑1 (MQ=255) cgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgag < 2:294404/138‑1 (MQ=255) ccAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGAACCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgaggag > 2:184276/1‑139 (MQ=255) ggCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTg < 2:84338/139‑1 (MQ=255) gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTg > 1:674957/1‑129 (MQ=255) gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATgc > 1:683981/1‑139 (MQ=255) gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAAGCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTg < 2:674957/129‑1 (MQ=255) gAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTgg > 1:327278/1‑139 (MQ=255) agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACg < 1:523904/92‑1 (MQ=255) agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACg > 2:523904/1‑92 (MQ=255) agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAgctgc > 1:214009/1‑130 (MQ=255) agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAgctgc < 2:214009/130‑1 (MQ=255) agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTgg > 1:138813/1‑139 (MQ=255) cggcgAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCg < 2:291528/139‑1 (MQ=255) gCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcc < 2:502197/139‑1 (MQ=255) tGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTg < 1:105070/90‑1 (MQ=255) tGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTg > 2:105070/1‑90 (MQ=255) gTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcctgcc < 2:683981/139‑1 (MQ=255) tttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATg < 1:346222/139‑1 (MQ=255) tttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGc < 1:634988/135‑1 (MQ=255) cGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAgc > 2:163606/1‑139 (MQ=255) cccGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGcc < 1:721143/139‑1 (MQ=255) cGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACg < 1:692898/61‑1 (MQ=255) cGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACg > 2:692898/1‑61 (MQ=255) cGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGa > 2:857204/1‑139 (MQ=255) caGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGAt > 1:682059/1‑138 (MQ=255) aGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTa < 1:857204/139‑1 (MQ=255) cgcCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATc > 2:94276/1‑139 (MQ=255) ccGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCg > 1:227856/1‑138 (MQ=255) gcgcCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGgc > 2:436006/1‑139 (MQ=255) cgcCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcctgcc < 2:374831/91‑1 (MQ=255) cgcCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcctgcc > 1:374831/1‑91 (MQ=255) | CACGCAGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGC > NZ_CP009273/509565‑509832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |