| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 524,350 | T→C | F85F (TTT→TTC) | BW25113_RS24960 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 524,350 | 0 | T | C | 100.0% | 100.1 / NA | 29 | F85F (TTT→TTC) | BW25113_RS24960 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/16); total (13/16) | |||||||||||
ACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTA > NZ_CP009273/524218‑524473 | aCATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTaa > 2:852524/1‑139 (MQ=255) cATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAatg < 1:401893/139‑1 (MQ=255) tACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGtt > 1:241134/1‑138 (MQ=255) gAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGa > 1:628698/1‑138 (MQ=255) aaaaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATCAAATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGa > 2:115237/1‑138 (MQ=255) aaaaTGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGaa > 1:315466/1‑138 (MQ=255) tgGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGa < 1:708070/139‑1 (MQ=255) gAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACa < 1:250607/139‑1 (MQ=255) tttttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg < 1:138090/139‑1 (MQ=255) tttttACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTg < 1:592941/139‑1 (MQ=255) cATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGcc < 2:315466/139‑1 (MQ=255) atC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAg < 1:110976/78‑1 (MQ=255) atC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAg > 2:110976/1‑78 (MQ=255) atC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGAtt > 1:673886/1‑135 (MQ=255) atC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATc > 2:14545/1‑136 (MQ=255) taCACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGt > 2:325058/1‑85 (MQ=255) taCACAAATGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGt < 1:325058/85‑1 (MQ=255) aTGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtg < 1:14545/139‑1 (MQ=255) tGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtg < 2:26733/138‑1 (MQ=255) tGTAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGCAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAgtg > 1:26733/1‑138 (MQ=255) tAGAAGATGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTgtgg > 2:348635/1‑139 (MQ=255) agaTGATTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTa < 1:802290/139‑1 (MQ=255) gctgatTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGt > 1:335815/3‑47 (MQ=255) gatgatTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGt < 2:335815/47‑1 (MQ=255) gatgatTATTTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTa > 1:334195/1‑138 (MQ=255) attattTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAatat < 1:852524/139‑1 (MQ=255) attattTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAatat < 2:334195/139‑1 (MQ=255) attattTTTTTCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAata < 2:458485/138‑1 (MQ=255) tttttttCTTATTTTCCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTa < 2:628698/139‑1 (MQ=255) | ACATTACATAAACTATATGGCAATACCAGAAAATGATGGAGTTTTTACATGGCTCCCAGATTTTTTTCCGCACGTAGCGGTGGATATATC‑AATATACACAAATGTAGAAGATGATTATTTTTTTCTTATTTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTA > NZ_CP009273/524218‑524473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |