Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	634,477	A→G	E26G (GAA→GGA)	ahpC →	alkyl hydroperoxide reductase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	634,477	0	A	G	100.0%	230.8 / NA	63	E26G (GAA→GGA)	ahpC	alkyl hydroperoxide reductase subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/34);  total (29/34)

AATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCA  >  NZ_CP009273/634347‑634610                                                                                                                                   |                                                                                                                                      aaTCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATa                                                                                                                               >  1:633629/1‑139 (MQ=255)       cAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAg                                                                                                                         >  1:506069/1‑139 (MQ=255)                gTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGa                                                                                                                <  2:352018/139‑1 (MQ=255)                               aTCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCACAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGtcttcttcttc                                                                                                  >  2:650529/1‑138 (MQ=255)                               aTCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCg                                                                                                             <  1:62991/127‑1 (MQ=255)                               aTCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCg                                                                                                             >  2:62991/1‑127 (MQ=255)                               aTCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGtcttcttcttc                                                                                                  >  1:168178/1‑138 (MQ=255)                               aTCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGtcttcttcttc                                                                                                  >  2:402009/1‑138 (MQ=255)                                      acacGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGc                                                                                          >  2:791360/1‑139 (MQ=255)                                              gaAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGacttta                                                                                  <  2:184098/139‑1 (MQ=255)                                                  tataGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCGCCGGAAAAGATACCGAAGGCCGCTGGAGCg                                                                                                             <  1:56171/108‑1 (MQ=255)                                                  tacaGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCg                                                                                                             >  2:56171/4‑108 (MQ=255)                                                         gTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGaa                                                                                                                          <  1:406487/88‑1 (MQ=255)                                                         gTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGaa                                                                                                                          >  2:406487/1‑88 (MQ=255)                                                         gTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTa                                                                                                <  1:40350/114‑1 (MQ=255)                                                         gTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTa                                                                                                >  2:40350/1‑114 (MQ=255)                                                         gTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATg                                                                        <  1:371808/138‑1 (MQ=255)                                                         gTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATg                                                                        >  2:371808/1‑138 (MQ=255)                                                          tCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCgg                                                                                           <  2:683900/118‑1 (MQ=255)                                                          tCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCgg                                                                                           >  1:683900/1‑118 (MQ=255)                                                           ccTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATg                                                                        <  1:791669/136‑1 (MQ=255)                                                           ccTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATg                                                                        >  2:791669/1‑136 (MQ=255)                                                           ccTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  1:602266/1‑139 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  2:209080/1‑138 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  2:864558/1‑138 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  2:97140/1‑138 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  2:140272/1‑138 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  2:118402/1‑138 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  1:526828/1‑138 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGccc                                                                     >  1:189868/1‑138 (MQ=255)                                                            cTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCCGACTTTACTTTCGTATGccc                                                                     >  2:184452/1‑138 (MQ=255)                                                               gATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTACTTCTTCTACCCGGCTGACTTTACTTTCGTATGCccgac                                                                  <  2:307519/138‑1 (MQ=255)                                                                   aaCACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGaa                                                              <  2:602266/138‑1 (MQ=255)                                                                       ccAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTggg                                                         <  2:506069/139‑1 (MQ=255)                                                                       ccAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTggg                                                         <  1:402009/139‑1 (MQ=255)                                                                        cAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGaa                                                                                                                          >  1:298922/1‑73 (MQ=255)                                                                        cAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGaa                                                                                                                          <  2:298922/73‑1 (MQ=255)                                                                            aTTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCCGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACg                                                    <  1:184452/139‑1 (MQ=255)                                                                                aaCCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTg                                                 <  2:633629/138‑1 (MQ=255)                                                                                     tttAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGAc                                            >  1:611715/1‑138 (MQ=255)                                                                                      ttAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGAc                                            <  1:118402/137‑1 (MQ=255)                                                                                      ttAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGAc                                            <  1:785905/137‑1 (MQ=255)                                                                                      ttAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGAc                                            <  1:436379/137‑1 (MQ=255)                                                                                      ttAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGAc                                            <  2:420801/137‑1 (MQ=255)                                                                                           aaCCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACg                                     <  1:140272/139‑1 (MQ=255)                                                                                             ccAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGAc                                            >  1:523786/1‑130 (MQ=255)                                                                                             ccAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGAc                                            <  2:523786/130‑1 (MQ=255)                                                                                             ccAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACg                                     >  2:460676/1‑137 (MQ=255)                                                                                              cAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACgaag                                  <  2:168178/139‑1 (MQ=255)                                                                                               aGGCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACgaaga                                 <  1:460676/139‑1 (MQ=255)                                                                                                ggCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGaa                                                              >  2:452614/1‑109 (MQ=255)                                                                                                ggCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGaa                                                              <  1:452614/109‑1 (MQ=255)                                                                                                ggCATTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACgaagaa                                >  1:124842/1‑139 (MQ=255)                                                                                                   aTTCAAAAACGGCGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTg                             <  1:864558/139‑1 (MQ=255)                                                                                                               cGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGa                                                      >  2:132914/1‑102 (MQ=255)                                                                                                               cGAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGa                                                      <  1:132914/102‑1 (MQ=255)                                                                                                                gAATTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCg                <  2:866616/139‑1 (MQ=255)                                                                                                                  aTTCATCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTa              <  2:404252/139‑1 (MQ=255)                                                                                                                       tCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGt         <  1:404108/139‑1 (MQ=255)                                                                                                                       tCGAAATCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGt         <  1:770651/139‑1 (MQ=255)                                                                                                                            aTCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACg    <  2:241301/139‑1 (MQ=255)                                                                                                                            aTCACCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACg    <  1:97140/139‑1 (MQ=255)                                                                                                                               aCCGGAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCa  <  1:791360/138‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      AATCGGCAAAAATTGGTTACCTTACATCTCATCGAAAACACGGAGGAAGTATAGATGTCCTTGATTAACACCAAAATTAAACCTTTTAAAAACCAGGCATTCAAAAACGGCGAATTCATCGAAATCACCGAAAAAGATACCGAAGGCCGCTGGAGCGTCTTCTTCTTCTACCCGGCTGACTTTACTTTCGTATGCCCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCA  >  NZ_CP009273/634347‑634610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑