Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	675,373	A→G	E137G (GAA→GGA)	ybeU →	DUF1266 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	675,373	0	A	G	100.0%	180.4 / NA	50	E137G (GAA→GGA)	ybeU	DUF1266 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/23);  total (27/23)

GGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAATCTTCTCAGTGCGAAGACAA  >  NZ_CP009273/675247‑675502                                                                                                                               |                                                                                                                                  ggTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGtt                                                                                                                        >  2:300093/1‑138 (MQ=255)                aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATcgcg                                                                                                        <  1:781586/139‑1 (MQ=255)                aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATcgcg                                                                                                        >  2:403751/1‑139 (MQ=255)                                        gCTGTGGGGAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTCCGAGCCCACCCTTa                                                                                >  2:39618/1‑139 (MQ=255)                                         cTGTGGGGAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTa                                                                                <  2:345872/138‑1 (MQ=255)                                          tgtgGGGAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                                                                          <  1:283772/127‑1 (MQ=255)                                          tgtgGGGAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                                                                          >  2:283772/1‑127 (MQ=255)                                            tgGGGAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTAt                                                                            >  1:767023/1‑139 (MQ=255)                                            tgGGGAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTAt                                                                            >  1:761572/1‑139 (MQ=255)                                             ggggAAGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTAt                                                                            >  1:49617/1‑138 (MQ=255)                                                 aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATcgcg                                                                                                        <  1:186138/106‑1 (MQ=255)                                                 aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATcgcg                                                                                                        >  2:186138/1‑106 (MQ=255)                                                 aaGGGGGAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAg                                                                       >  1:698303/1‑139 (MQ=255)                                                    ggggAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCaa                                                                                                             <  2:122452/98‑1 (MQ=255)                                                    ggggAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCaa                                                                                                             >  1:122452/1‑98 (MQ=255)                                                      ggAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGa                                                                  >  1:683159/1‑139 (MQ=255)                                                      ggAACCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGa                                                                  <  1:39618/139‑1 (MQ=255)                                                        aaccAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                                                                >  2:524454/4‑139 (MQ=255)                                                        aaccAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                                                                <  2:468195/136‑1 (MQ=255)                                                          ccAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCa                                                              <  2:767023/138‑1 (MQ=255)                                                              aGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGttt                                                                                                                       >  2:460691/1‑78 (MQ=255)                                                              aGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGttt                                                                                                                       <  1:460691/78‑1 (MQ=255)                                                                   gggATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                                                     <  1:463722/91‑1 (MQ=255)                                                                   gggATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                                                     >  2:463722/1‑91 (MQ=255)                                                                    ggATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTcc                                                    >  2:386723/1‑139 (MQ=255)                                                                    ggATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTcc                                                    <  2:606143/139‑1 (MQ=255)                                                                          tGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTa                                               <  2:683159/138‑1 (MQ=255)                                                                          tGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTa                                               <  1:754966/138‑1 (MQ=255)                                                                                 aTGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGTATGCACTATTTTTCCGCCTATTCGTTAg                                       <  1:524454/139‑1 (MQ=255)                                                                                 aTGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAg                                       >  1:754594/1‑139 (MQ=255)                                                                                 aTGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAg                                       <  1:687687/139‑1 (MQ=255)                                                                                 aTGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAg                                       <  1:118771/139‑1 (MQ=255)                                                                                             tCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTAc                            <  2:648648/138‑1 (MQ=255)                                                                                             tCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTAc                            <  1:138486/138‑1 (MQ=255)                                                                                               aCGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTg                          <  2:49617/138‑1 (MQ=255)                                                                                                cGGGTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTgg                         >  1:100124/1‑138 (MQ=255)                                                                                                   gTCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAAt                     >  1:705815/1‑139 (MQ=255)                                                                                                    tCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAAtc                    >  1:728299/1‑139 (MQ=255)                                                                                                    tCGGTGTGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAAtc                    >  2:596445/1‑139 (MQ=255)                                                                                                       gtgtGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                                                                >  1:792232/1‑92 (MQ=255)                                                                                                       gtgtGCTCAACAAGTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                                                                <  2:792232/92‑1 (MQ=255)                                                                                                                    gTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAATCTTCTCAGTGCGAAGa     >  2:679371/1‑138 (MQ=255)                                                                                                                    gTGGCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAATCTTCTCAGTGCGAAGa     >  1:347001/1‑138 (MQ=255)                                                                                                                       gCTGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAATCTTCTCAGTGCGAAGacaa  >  2:795267/1‑138 (MQ=255)                                                                                                                         tGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                                                     >  2:101315/1‑37 (MQ=255)                                                                                                                         tGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                                                     <  1:101315/37‑1 (MQ=255)                                                                                                                         tGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAATCTTCTCAGTGc          <  2:6166/128‑1 (MQ=255)                                                                                                                         tGACGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAATCTTCTCAGTGc          >  1:6166/1‑128 (MQ=255)                                                                                                                           aCGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTCCTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTg                          <  2:461635/110‑1 (MQ=255)                                                                                                                           aCGGGAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTg                          >  1:461635/1‑110 (MQ=255)                                                                                                                               |                                                                                                                                  GGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAGGACGTCTCTACTGGCAATCTTCTCAGTGCGAAGACAA  >  NZ_CP009273/675247‑675502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑