Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	716,126	A→G	intergenic (‑210/+48)	speF ← / ← BW25113_RS26090	ornithine decarboxylase SpeF/leader peptide SpeFL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	716,126	0	A	G	100.0%	128.9 / NA	34	intergenic (‑210/+48)	speF/BW25113_RS26090	ornithine decarboxylase SpeF/leader peptide SpeFL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

GCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATACAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGCCTTATATGGGG  >  NZ_CP009273/715990‑716254                                                                                                                                         |                                                                                                                                 gCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCa                                                                                                                                <  2:138352/139‑1 (MQ=255) gCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCa                                                                                                                                <  1:36735/139‑1 (MQ=255) gCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCa                                                                                                                                <  1:794653/139‑1 (MQ=255) gCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCa                                                                                                                                <  1:772323/139‑1 (MQ=255)            ccGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTT‑ACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGaa                                                                                                                    <  2:577484/139‑1 (MQ=255)                       taaatCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACg                                                                                                          <  1:3861/139‑1 (MQ=255)                                    ttGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCtt                                                                                             <  1:766097/139‑1 (MQ=255)                                             tCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTa                                                                                     <  1:145289/138‑1 (MQ=255)                                                 aaTGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGAc                                                                                                                 >  1:533265/1‑106 (MQ=255)                                                 aaTGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGAc                                                                                                                 <  2:533265/106‑1 (MQ=255)                                                              ttACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTg                                                                      >  2:18108/1‑136 (MQ=255)                                                                     tCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAg                                                                                                    >  1:4630/1‑99 (MQ=255)                                                                     tCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAg                                                                                                    <  2:4630/99‑1 (MQ=255)                                                                      cAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGCGCATTGAAGAAGTGa                                                             >  2:460184/1‑137 (MQ=255)                                                                              tGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGc                                                   >  1:531180/1‑139 (MQ=255)                                                                                              aaCAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTgaa                                                                    >  1:680021/1‑106 (MQ=255)                                                                                              aaCAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTgaa                                                                    <  2:680021/106‑1 (MQ=255)                                                                                              aaCAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCaaa                                   >  2:728625/1‑139 (MQ=255)                                                                                                   agaATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCa                              >  2:593587/1‑139 (MQ=255)                                                                                                    gaATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAg                                       <  1:608754/129‑1 (MQ=255)                                                                                                    gaATGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAg                                       >  2:608754/1‑129 (MQ=255)                                                                                                     aaTGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCat                             >  2:754040/1‑138 (MQ=255)                                                                                                     aaTGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCat                             >  2:338974/1‑138 (MQ=255)                                                                                                     aaTGAGCACTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCat                             >  1:22330/1‑138 (MQ=255)                                                                                                             cTGCGGAATACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGt                    >  2:420541/1‑139 (MQ=255)                                                                                                                 gtaaTACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGCCCTGTACGCTGAAGGGCCCTTAGTCGTTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTc                <  2:703481/137‑1 (MQ=255)                                                                                                                     tACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGcc            <  2:385918/139‑1 (MQ=255)                                                                                                                     tACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGcc            <  2:22330/139‑1 (MQ=255)                                                                                                                     tACAGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGcc            <  1:618559/139‑1 (MQ=255)                                                                                                                        aGGCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGCCTta         >  1:103234/1‑139 (MQ=255)                                                                                                                          gCATATGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGCCTtata       <  1:754040/139‑1 (MQ=255)                                                                                                                               tGCCTGTAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGCCTTATATgggg  >  1:752281/1‑139 (MQ=255)                                                                                                                                     tAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTc                >  2:575538/1‑119 (MQ=255)                                                                                                                                     tAATGCAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTc                <  1:575538/119‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 GCAAAGTTACCCCGCTGATAAATAAATCAGGTAGATTGCTTTTTTCAGAATGCAGAAACCCTTACCAGTCAGGTGGCTGATAATACAAGGGTTAACAGAGAATGAGCACTGCGGAATACAGGCATATGCCTGTAATACAGAGAGTCAGGAAGACCTGTACGCTGAAGTGCCCTTAGTCGGTAGACTAACGGGCATTGAAGAAGTGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGAGTTGTCCGCCTTATATGGGG  >  NZ_CP009273/715990‑716254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑