Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 757,924 T→C L316P (CTG→CCG)  odhB → 2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273757,9240TC100.0% 131.8 / NA 39L316P (CTG→CCG) odhB2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/22);  total (17/22)

TTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGT  >  NZ_CP009273/757795‑758057
                                                                                                                                 |                                                                                                                                     
tttCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCggggg                                                                                                                              >  2:483073/1‑139 (MQ=255)
         cgcgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtcac                                                                                                                     >  1:533098/1‑139 (MQ=255)
          gcgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtcacc                                                                                                                    <  1:4275/139‑1 (MQ=255)
           cgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGa                                                                                                                                     >  1:739120/1‑121 (MQ=255)
           cgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGa                                                                                                                                     <  2:739120/121‑1 (MQ=255)
           cgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtca                                                                                                                      >  2:606252/1‑136 (MQ=255)
           cgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtca                                                                                                                      <  1:606252/136‑1 (MQ=255)
             cgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtcaccatc                                                                                                                 <  1:472051/139‑1 (MQ=255)
                          ccGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtca                                                                                                                      <  2:632714/121‑1 (MQ=255)
                          ccGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtca                                                                                                                      >  1:632714/1‑121 (MQ=255)
                          ccGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGgtggtg                                                                                                    >  1:79752/1‑139 (MQ=255)
                                   cGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCgg                                                                                             <  2:95327/137‑1 (MQ=255)
                                   cGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCg                                                                                              <  2:315634/136‑1 (MQ=255)
                                       aTGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCt                                                                                       <  2:79752/139‑1 (MQ=255)
                                        tGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCt                                                                                       >  1:360367/1‑138 (MQ=255)
                                                   tCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGcc                                                                           >  1:378319/1‑139 (MQ=255)
                                                    cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACg                                                                             >  2:786018/1‑136 (MQ=255)
                                                    cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGcc                                                                           >  2:721604/1‑138 (MQ=255)
                                                     ggCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGa                                                                         >  1:750947/1‑139 (MQ=255)
                                                           tcAGGCAGCGAGAAGAAAAGCAAAGAGCTGGCAGACAAAGGCCGTGACGGCAAGCTGACCGTGGAAGATCCGCCCGGTGGTAACTTCACCATCACCAACGGTGGGGTGTTCGGTCCCCTGATGTCTCGGCCGatcacaa                                                                   <  2:616265/138‑4 (MQ=255)
                                                           gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTa                                                                               >  2:709020/1‑127 (MQ=255)
                                                           gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTa                                                                               >  2:806048/1‑127 (MQ=255)
                                                           gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTa                                                                               <  1:806048/127‑1 (MQ=255)
                                                           gCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTa                                                                               <  1:709020/127‑1 (MQ=255)
                                                            cAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCaa                                                                  >  1:29431/1‑139 (MQ=255)
                                                                         gaaAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAgc                                                     <  1:211252/139‑1 (MQ=255)
                                                                                aaaGAGCGGGCAGTCAAAGGCGGTGACGGCAAGCTGACGGGTGAAGTCCCGACCGGTGGTAACTCCACCATCACCAACGGGGTTGTTTTGGGTTCCCTGATGTTTACGCCGATCATCACCCCGCCGCAGAGCGCAATTc                                              <  1:387929/139‑1 (MQ=255)
                                                                                          cAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCa                                    <  2:360367/139‑1 (MQ=255)
                                                                                            gtaaaaGGCCGTGACGGCAAGCTGCCCGTTGAAGACCCGCCCGGTGGTAACTTCCCCATCACCACCGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACg                                  <  2:378319/136‑1 (MQ=255)
                                                                                            gTCAAAGGCCGTGACGGCAAGCTGCCCGTTGAAGATCCGACCGGTGGTACCTTCCCCATCACCAACGGTGGTGTGTTCGTTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACg                                  <  2:204392/139‑1 (MQ=255)
                                                                                            gTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACg                                  <  1:721604/139‑1 (MQ=255)
                                                                                            gTCAAAGGCCGTGACGCCAAGCTGACCGTTGAATATCCGCCCGGTGGTAACTTCACCATCACCAACGGTGGTGTTTTCGTTTCCCTGATGTCTACCCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACg                                  <  1:459107/139‑1 (MQ=255)
                                                                                                       tGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCt                                                                                       >  1:798715/1‑75 (MQ=255)
                                                                                                       tGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCt                                                                                       <  2:798715/75‑1 (MQ=255)
                                                                                                               aGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCg                                                                          <  2:477794/80‑1 (MQ=255)
                                                                                                               aGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCg                                                                          >  1:477794/1‑80 (MQ=255)
                                                                                                                    aCCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCgg          >  1:852660/1‑139 (MQ=255)
                                                                                                                        ttGAAGATCCGCCCGGTGGTAACTTAACAATCACCAAGGGTGGGGTGTGCGGTTCCCGGATGTCTACGCCGATCATAAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGaa      <  2:182633/139‑1 (MQ=255)
                                                                                                                            aGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGt  <  1:370616/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                     
TTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGT  >  NZ_CP009273/757795‑758057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑