Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 781,531 T→C I148T (ATC→ACC)  aroG → 3‑deoxy‑7‑phosphoheptulonate synthase AroG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273781,5310TC100.0% 150.5 / NA 42I148T (ATC→ACC) aroG3‑deoxy‑7‑phosphoheptulonate synthase AroG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

GGCTGGAAAGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGATCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGG  >  NZ_CP009273/781395‑781660
                                                                                                                                        |                                                                                                                                 
ggCTGGAAAGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCa                                                                                                                                 <  1:229323/139‑1 (MQ=255)
ggCTGGAAAGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCa                                                                                                                                 >  1:383439/1‑139 (MQ=255)
 gCTGGAAAGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAc                                                                                                                                >  1:229153/1‑139 (MQ=255)
     gAAAGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGaccacc                                                                                                                               >  2:56806/1‑136 (MQ=255)
     gAAAGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGaccacc                                                                                                                               <  1:56806/136‑1 (MQ=255)
        aGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCa                                                                                                                                 <  2:90830/131‑1 (MQ=255)
               aTTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCg                                                                                                                  >  1:475006/1‑139 (MQ=255)
                         cGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtgat                                                                                                        >  1:370542/1‑139 (MQ=255)
                                   taataGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGgc                                                                                              >  1:519685/1‑139 (MQ=255)
                                     ataGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCca                                                                                                                            <  2:863872/107‑1 (MQ=255)
                                     ataGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCca                                                                                                                            >  1:863872/1‑107 (MQ=255)
                                     ataGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGgcg                                                                                             <  1:156638/138‑1 (MQ=255)
                                      taGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGgcgc                                                                                            >  2:701923/1‑138 (MQ=255)
                                         cTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAAtt                                                                                        >  1:101239/1‑139 (MQ=255)
                                         cTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAAtt                                                                                        >  1:683075/1‑139 (MQ=255)
                                         cTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAAtt                                                                                        >  1:597388/1‑139 (MQ=255)
                                         cTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAAtt                                                                                        <  2:229153/139‑1 (MQ=255)
                                           tCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTgg                                                                                      >  1:406432/1‑139 (MQ=255)
                                            ccAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCa                                                                                                                                 >  2:309083/1‑95 (MQ=255)
                                            ccAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCa                                                                                                                                 <  1:309083/95‑1 (MQ=255)
                                                   aaCGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTa                                                                              >  2:82274/1‑139 (MQ=255)
                                                       acgGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTaccac                                                                          <  1:82274/139‑1 (MQ=255)
                                                                   taGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGt                                                              <  2:275875/139‑1 (MQ=255)
                                                                    atcccGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTg                                                             >  2:461240/3‑139 (MQ=255)
                                                                    aGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTg                                                             >  1:205301/1‑139 (MQ=255)
                                                                     gCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGAcc                                                                                                            >  1:458007/1‑91 (MQ=255)
                                                                     gCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGAcc                                                                                                            <  2:458007/91‑1 (MQ=255)
                                                                         gTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACcg                                                        >  1:525160/1‑139 (MQ=255)
                                                                             aTTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGaa                                                    <  1:108483/139‑1 (MQ=255)
                                                                                 ctgctTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTgg                                                >  1:531069/1‑139 (MQ=255)
                                                                                    ctTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCa                                              <  2:490813/138‑1 (MQ=255)
                                                                                    ctTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCAt                                             <  2:591049/139‑1 (MQ=255)
                                                                                         tatTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAggg                                        <  2:200584/139‑1 (MQ=255)
                                                                                              aCGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTc                                   >  1:638234/1‑139 (MQ=255)
                                                                                                   aGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAata                                                                                                                       >  1:832309/1‑50 (MQ=255)
                                                                                                   aGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAata                                                                                                                       <  2:832309/50‑1 (MQ=255)
                                                                                                   aGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGgcg                                                                                             <  2:238706/76‑1 (MQ=255)
                                                                                                   aGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGgcg                                                                                             >  1:238706/1‑76 (MQ=255)
                                                                                                   aGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTc                              >  1:179387/1‑139 (MQ=255)
                                                                                                   aGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTc                              >  1:796546/1‑139 (MQ=255)
                                                                                                        tCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTc                         >  2:196694/1‑139 (MQ=255)
                                                                                                                      gTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATgg           <  2:101239/139‑1 (MQ=255)
                                                                                                                                 gATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACgg  <  2:745962/137‑1 (MQ=255)
                                                                                                                                 gATATGACCACCCCACAAT‑TCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACgg  <  2:361641/136‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GGCTGGAAAGGGCTGATTAACGATCCGCATATGGATAATAGCTTCCAGATCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGATCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTGGCATCAGGGCTTTCTTGTCCGGTCGGCTTCAAAAATGGCACCGACGG  >  NZ_CP009273/781395‑781660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑