| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 800,612 | A→C | T552P (ACC→CCC) | ybhJ → | hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 800,612 | 0 | A | C | 84.6% | 100.9 / 10.8 | 39 | T552P (ACC→CCC) | ybhJ | hydratase |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (17/16); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01 | |||||||||||
AGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAAT > NZ_CP009273/800478‑800743 | aGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCcg < 2:198715/138‑1 (MQ=255) tgtgAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACt > 1:147116/1‑138 (MQ=255) tgtgAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACt > 1:491774/1‑138 (MQ=255) ggggCAACTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGAttcctt > 1:364278/1‑139 (MQ=255) aaCTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGt > 2:186546/1‑139 (MQ=255) aaCTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGt < 2:215255/139‑1 (MQ=255) cTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTg > 1:605150/1‑138 (MQ=255) aGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGTTTCCTTCCGGTGAAAc < 1:124902/139‑1 (MQ=255) aGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAAc > 1:75073/1‑139 (MQ=255) cAACCGCTGATTTACGGGCCGAACAATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAAcc > 2:56131/1‑139 (MQ=255) cAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACct > 1:523711/1‑139 (MQ=255) cTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCtcttct > 2:604001/1‑138 (MQ=255) tACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGt < 2:75073/138‑1 (MQ=255) ggCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCaaa < 2:764026/139‑1 (MQ=255) cGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATcc > 2:822644/1‑139 (MQ=255) aC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGAt < 1:822644/139‑1 (MQ=255) aC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGAt < 2:666290/139‑1 (MQ=255) aaaGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGt < 2:605150/138‑1 (MQ=255) gACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTgg < 1:56131/139‑1 (MQ=255) aCTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGc < 2:794837/139‑1 (MQ=255) cTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCg > 2:317436/1‑139 (MQ=255) ggCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCcga < 1:807646/79‑1 (MQ=255) ggCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCcga > 2:807646/1‑79 (MQ=255) ggCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCg > 1:558440/1‑137 (MQ=255) ggCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCg > 2:585864/1‑137 (MQ=255) ggCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCg > 1:639252/1‑137 (MQ=255) ccGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACTTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGt < 2:251714/139‑1 (MQ=255) gcTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGaaa > 1:546329/1‑86 (MQ=255) gcTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGaaa < 2:546329/86‑1 (MQ=255) tCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAATGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTa > 2:243203/1‑139 (MQ=255) gTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGa < 1:753295/55‑1 (MQ=255) gTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGa > 2:753295/1‑55 (MQ=255) gTCCTGAAAGTGTGCTCGAAGAGCCTCGACGAAGTGACCCCCACCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTAt < 2:109063/138‑1 (MQ=255) aaaGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCa < 1:562666/139‑1 (MQ=255) aaaGTGTGCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCa < 1:24019/139‑1 (MQ=255) tgCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGt > 2:24026/1‑138 (MQ=255) gCTCGAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTa > 1:246128/1‑138 (MQ=255) gAAGATCCTCGACGAAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAg < 1:367897/137‑1 (MQ=255) gacgaAGTGACCACCCCCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGc < 1:740128/139‑1 (MQ=255) accacccccGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAAt < 2:523711/138‑1 (MQ=255) accacccccGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAAt > 2:333008/1‑138 (MQ=255) | AGGGCTTTGTGAAAGGGGCAACTCAGCAACCGCTGATTTACGGGCCGAAC‑ATTAAAGACTGGCCGGAATTGGGTGCGCTGACTGACAATATCGTCCTGAAAGTGTGCTCGAAGATCCTCGACGAAGTGACCACCACCGACGAACTGATTCCTTCCGGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAAT > NZ_CP009273/800478‑800743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |