BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	834,220	A→G	D148D (GAT→GAC)	ybiX ←	PKHD‑type hydroxylase YbiX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	834,220	0	A	G	100.0%	119.6 / NA	35	D148D (GAT→GAC)	ybiX	PKHD‑type hydroxylase YbiX
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/17); total (18/17)

TTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTG  >  NZ_CP009273/834086‑834350

ttgtCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCg                                                                                                                                 <  2:302535/138‑1 (MQ=255)

   tCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTg                                                                                                                             >  1:2243/1‑139 (MQ=255)

        ttCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCgg                                                                                                                         >  1:728576/1‑138 (MQ=255)

        ttCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCgg                                                                                                                         >  1:64507/1‑138 (MQ=255)

            aaCAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGtt                                                                                                                    <  2:2243/139‑1 (MQ=255)

                   tGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTAccc                                                                                                              >  1:75293/1‑138 (MQ=255)

                      cgcgcTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtg                                                                                                          <  2:781976/139‑1 (MQ=255)

                      cgcgcTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtg                                                                                                          >  2:307581/1‑139 (MQ=255)

                                 tcatcGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAg                                                                                                <  2:147339/138‑1 (MQ=255)

                                   atcGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGgt                                                                                              <  1:694581/138‑1 (MQ=255)

                                     cgcgGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGgtgt                                                                                            >  1:116662/1‑138 (MQ=255)

                                               cGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGAc                                                                                  >  2:393770/1‑138 (MQ=255)

                                                    ggATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTc                                                                            >  1:294716/1‑139 (MQ=255)

                                                     gATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcg                                                                           >  1:301812/1‑139 (MQ=255)

                                                       tCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgcc                                                                         <  2:274668/139‑1 (MQ=255)

                                                         cacaTAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgccg                                                                        <  2:339189/138‑1 (MQ=255)

                                                               aaTGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCcgtcg                                                                  >  2:364503/1‑138 (MQ=255)

                                                                atgatgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                                <  2:294716/139‑1 (MQ=255)

                                                                     gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtg                                                                                                          <  1:266019/92‑1 (MQ=255)

                                                                     gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtg                                                                                                          >  2:266019/1‑92 (MQ=255)

                                                                     gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCGGCCGTCGTAGCttt                                                           >  1:433562/1‑139 (MQ=255)

                                                                     gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCt                                                             >  1:375706/1‑137 (MQ=255)

                                                                     gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCt                                                             <  2:375706/137‑1 (MQ=255)

                                                                         cTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtgtg                                                        >  1:670713/1‑138 (MQ=255)

                                                                          tCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGa                                                      >  2:565370/1‑139 (MQ=255)

                                                                                   gcgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaa                                              >  2:499894/1‑138 (MQ=255)

                                                                                    cgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaa                                             >  1:602078/1‑138 (MQ=255)

                                                                                           cGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCg                                      <  1:448069/138‑1 (MQ=255)

                                                                                           cGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCg                                      <  2:64507/138‑1 (MQ=255)

                                                                                           cGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCg                                      <  2:728576/138‑1 (MQ=255)

                                                                                                 tCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGa                               <  2:670713/139‑1 (MQ=255)

                                                                                                      cAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGt                          <  1:41740/139‑1 (MQ=255)

                                                                                                                  ctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATc               >  1:844877/1‑138 (MQ=255)

                                                                                                                      aGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGcc          <  1:364503/139‑1 (MQ=255)

                                                                                                                              aacaCGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTtgtg  <  1:499894/139‑1 (MQ=255)

                                                                                                                                   gaggTCGCCTGCCGGGAGTTTTACCCGATGTTGACCGAAGGTGTCATTAACGAc                                                                                  >  1:425249/1‑54 (MQ=255)

                                                                                                                                   gaggTCGCCTGCCGGGAGTTTTACCCGATGTTGACCGAAGGTGTCATTAACGAc                                                                                  <  2:425249/54‑1 (MQ=255)

TTGTCCAGTTCAAACAGCATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTG  >  NZ_CP009273/834086‑834350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`