Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 861,316 T→C I169V (ATT→GTT)  moeA ← molybdopterin molybdotransferase MoeA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273861,3160TC90.3% 85.5 / 3.0 31I169V (ATT→GTT) moeAmolybdopterin molybdotransferase MoeA
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (15/13);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCA  >  NZ_CP009273/861184‑861450
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aCATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAAcccc                                                                                                                                     <  1:705217/136‑1 (MQ=255)
aCATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAg                                                                                                                                  >  2:528030/1‑139 (MQ=255)
     aGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg                                                                                                                             >  2:600752/1‑139 (MQ=255)
     aGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg                                                                                                                             >  1:41485/1‑139 (MQ=255)
     aGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg                                                                                                                             >  1:810035/1‑139 (MQ=255)
               cGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTgg                                                                                                                   <  1:67411/139‑1 (MQ=255)
                 agaCGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTgg                                                                                                                   >  2:843076/1‑137 (MQ=255)
                  gaCGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAg                                                                                                                >  1:233948/1‑139 (MQ=255)
                  gaCGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAg                                                                                                                <  1:600752/139‑1 (MQ=255)
                             tCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAg                                                                                                     <  1:743966/139‑1 (MQ=255)
                               gTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGt                                                                                                                                 <  1:255455/109‑1 (MQ=255)
                               gTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGt                                                                                                                                 >  2:255455/1‑109 (MQ=255)
                                    tttGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGc                                                                                               >  2:695101/1‑138 (MQ=255)
                                          cGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTcc                                                                                        >  2:174679/1‑139 (MQ=255)
                                                   gCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGaa                                                                               <  1:174679/139‑1 (MQ=255)
                                                      gCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCg                                                                                                         <  2:250246/110‑1 (MQ=255)
                                                      gCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCg                                                                                                         >  1:250246/1‑110 (MQ=255)
                                                                 aaCTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTg                                                                 <  2:389591/139‑1 (MQ=255)
                                                                 aaCTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTg                                                                 <  2:715828/139‑1 (MQ=255)
                                                                      gAGTTCATCACCGGTAGAAAAAGGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCaga                                                             <  2:205727/138‑1 (MQ=255)
                                                                         ttCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGata                                                         >  1:500191/1‑139 (MQ=255)
                                                                              caCCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTTGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCtt                                                     >  2:101477/1‑138 (MQ=255)
                                                                                  ggTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACcg                                                >  2:362551/1‑139 (MQ=255)
                                                                                    tAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACcgcg                                              <  1:695101/139‑1 (MQ=255)
                                                                                              cgcTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAg                                                                                                                >  1:783785/1‑63 (MQ=255)
                                                                                              cgcTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAg                                                                                                                <  2:783785/63‑1 (MQ=255)
                                                                                                 tACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATAttt                                 >  1:140145/1‑139 (MQ=255)
                                                                                                    acGCATTTGTCGAATACCCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGTGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGc                              <  2:864225/139‑1 (MQ=255)
                                                                                                                    aCCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTcag              <  1:362551/139‑1 (MQ=255)
                                                                                                                              cGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAacg    >  2:857865/1‑139 (MQ=255)
                                                                                                                                gCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAacgca  >  1:662134/1‑139 (MQ=255)
                                                                                                                                gCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAacgca  <  1:742453/139‑1 (MQ=255)
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ACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCA  >  NZ_CP009273/861184‑861450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑