Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	60,340	T→C	M3V (ATG→GTG)	rluA ←	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	60,340	0	T	C	100.0%	183.4 / NA	51	M3V (ATG→GTG)	rluA	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/20);  total (31/20)

CACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAA  >  NZ_CP009273/60209‑60473                                                                                                                                    |                                                                                                                                      caCGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCAtt                                                                                                                                >  2:118391/1‑139 (MQ=255)        tCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCgg                                                                                                                           <  2:87552/137‑1 (MQ=255)        tCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCgg                                                                                                                           <  1:415111/137‑1 (MQ=255)        tCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCt                                                                                                                         >  2:848936/1‑139 (MQ=255)            tgtgCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTc                                                                                                                        <  1:658034/136‑1 (MQ=255)                  ctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGa                                                                                                               >  1:344692/1‑139 (MQ=255)                  ctTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGa                                                                                                               >  1:426043/1‑139 (MQ=255)                   ttCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTg                                                                                                                <  1:861641/137‑1 (MQ=255)                             cccGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAAc                                                                                                    >  1:133775/1‑139 (MQ=255)                                       acaacaAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACg                                                                                           >  2:122992/1‑138 (MQ=255)                                                 gCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATg                                                                                                             >  2:451755/1‑110 (MQ=255)                                                 gCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATg                                                                                                             <  1:451755/110‑1 (MQ=255)                                                 gCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATc                                                                                  >  2:677986/1‑137 (MQ=255)                                                  cTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAGCCAACCAGGGTACCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGACTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAg                                                                               <  2:222632/139‑1 (MQ=255)                                                   tCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGa                                                                              >  2:287734/1‑139 (MQ=255)                                                    cGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGAc                                                                             >  2:836285/1‑139 (MQ=255)                                                          gttgACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAc                                                                       >  1:250379/1‑139 (MQ=255)                                                           ttgACCACCAGAATATTGTCATCCTGATCCACGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCATACGCCAAc                                                                       <  2:143443/138‑1 (MQ=255)                                                              accaccATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcctgc                                                                   <  1:118391/139‑1 (MQ=255)                                                               ccaccaTAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcctgcc                                                                  <  1:469016/139‑1 (MQ=255)                                                                 accaTAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcctgcct                                                                 >  2:557673/1‑138 (MQ=255)                                                                  ccaTAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGa                                                               >  2:738824/1‑139 (MQ=255)                                                                        tatGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAgg                                                         >  2:540494/1‑139 (MQ=255)                                                                        tatGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAgg                                                         <  2:250379/139‑1 (MQ=255)                                                                            gTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCttt                                                     >  1:102546/1‑139 (MQ=255)                                                                             tCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGa                                                                                                               <  1:658450/80‑1 (MQ=255)                                                                             tCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGa                                                                                                               >  2:658450/1‑80 (MQ=255)                                                                             tCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATTCAGACGCCAACCTGCCTGATCCAGGCTTTc                                                    >  1:750823/1‑139 (MQ=255)                                                                             tCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATc                                                             >  2:260683/1‑130 (MQ=255)                                                                             tCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATc                                                             <  1:260683/130‑1 (MQ=255)                                                                             tCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTc                                                    >  1:782402/1‑139 (MQ=255)                                                                             tCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTc                                                    >  1:298264/1‑139 (MQ=255)                                                                                tCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCAt                                                 >  1:67817/1‑139 (MQ=255)                                                                                     aTACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTAc                                              <  1:170141/137‑1 (MQ=255)                                                                                        cAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACctgct                                         <  1:677986/139‑1 (MQ=255)                                                                                           gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcctgc                                                                   >  1:452259/1‑110 (MQ=255)                                                                                           gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAAcctgc                                                                   <  2:452259/110‑1 (MQ=255)                                                                                           gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGAc                                      >  1:173349/1‑139 (MQ=255)                                                                                           gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGAc                                      >  2:461761/1‑139 (MQ=255)                                                                                               accaaccaGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGt                                   <  1:848936/138‑1 (MQ=255)                                                                                                     caGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACgg                                    >  2:70202/1‑131 (MQ=255)                                                                                                     caGGGTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACgg                                    <  1:70202/131‑1 (MQ=255)                                                                                                        ggTTCCTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAAt                         >  1:716025/1‑139 (MQ=255)                                                                                                             cTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATcc                                                                                 >  2:672810/1‑78 (MQ=255)                                                                                                             cTGCGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATcc                                                                                 <  1:672810/78‑1 (MQ=255)                                                                                                                cGGTGGATTGTAGTTTTCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGt                   <  2:102546/137‑1 (MQ=255)                                                                                                                               ttCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAgg                                                         >  2:333241/1‑84 (MQ=255)                                                                                                                               ttCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAgg                                                         <  1:333241/84‑1 (MQ=255)                                                                                                                               ttCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGaa  >  2:390460/1‑139 (MQ=255)                                                                                                                               ttCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGaa  >  2:652994/1‑139 (MQ=255)                                                                                                                               ttCCACCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGaa  >  1:106295/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      CACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCGCTCGGCTTGTTGACCACCATAATATGGTCATCCTGATACAGGATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAA  >  NZ_CP009273/60209‑60473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑