Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 928,200 T→C R50R (CGT→CGC)  lrp → leucine‑responsive transcriptional regulator Lrp

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273928,2000TC100.0% 140.7 / NA 41R50R (CGT→CGC) lrpleucine‑responsive transcriptional regulator Lrp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/22);  total (19/22)

GCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATAC  >  NZ_CP009273/928070‑928334
                                                                                                                                  |                                                                                                                                      
gcCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGa                                                                                                                                <  2:378984/139‑1 (MQ=255)
  ccTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCACCGCCGTGCCTTGAGCGTGTGCGCCGGCTGGa                                                                                                                                >  2:743798/1‑137 (MQ=255)
           gATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                     <  2:369800/139‑1 (MQ=255)
           gATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                     <  1:630789/139‑1 (MQ=255)
           gATCTCGACCGCATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                     <  1:252376/139‑1 (MQ=255)
            aTCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGtt                                                                                                                    >  1:133680/1‑139 (MQ=255)
                cGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                     >  2:585563/1‑134 (MQ=255)
                cGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                     <  1:585563/134‑1 (MQ=255)
                 gACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTAtt                                                                                                                <  1:294281/138‑1 (MQ=255)
                 gACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTAtt                                                                                                                <  2:691907/138‑1 (MQ=255)
                     gTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAggg                                                                                                           <  2:133680/139‑1 (MQ=255)
                      tATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                                                                               <  1:78065/134‑1 (MQ=255)
                      tATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTc                                                                                                               >  2:78065/1‑134 (MQ=255)
                          gatcgTAACATACTTAATGAGTTGCAAAAGGATGGGCGGATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCtata                                                                                                      <  1:383558/139‑1 (MQ=255)
                                      cTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCtt                                                                                           >  1:104146/1‑138 (MQ=255)
                                      cTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCtt                                                                                           >  2:196483/1‑138 (MQ=255)
                                      cTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCtt                                                                                           >  1:283187/1‑138 (MQ=255)
                                         aaTGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTaa                                                                                         <  2:104146/137‑1 (MQ=255)
                                                            ggCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCAt                                                                     >  2:640713/1‑138 (MQ=255)
                                                                              aGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGtt                                                    >  2:219615/1‑137 (MQ=255)
                                                                                cTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTgaga                                                <  2:283187/139‑1 (MQ=255)
                                                                                       aaCGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTAct                                           <  1:219615/137‑1 (MQ=255)
                                                                                             tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGa                                                                          <  2:249944/100‑1 (MQ=255)
                                                                                             tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGa                                                                          >  1:249944/1‑100 (MQ=255)
                                                                                             tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTgaga                                                >  1:516228/1‑126 (MQ=255)
                                                                                             tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTgaga                                                <  2:516228/126‑1 (MQ=255)
                                                                                             tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCg                                   >  2:59973/1‑139 (MQ=255)
                                                                                                gACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTgg                                <  1:681168/139‑1 (MQ=255)
                                                                                                gACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTgg                                <  1:732807/139‑1 (MQ=255)
                                                                                                                 tGCCTTGAGCGTGTGCGCCGGCTGGACAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGa                                                                          >  2:488850/1‑80 (MQ=255)
                                                                                                                 tGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGa                                                                          <  1:488850/80‑1 (MQ=255)
                                                                                                                 tGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGttt                >  2:790652/1‑138 (MQ=255)
                                                                                                                   ccTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGaa             <  1:749578/139‑1 (MQ=255)
                                                                                                                      tGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACaa          >  1:644382/1‑139 (MQ=255)
                                                                                                                           gtgtGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcg                                                                                                 >  2:554520/1‑47 (MQ=255)
                                                                                                                           gtgtGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcg                                                                                                 <  1:554520/47‑1 (MQ=255)
                                                                                                                             gtgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcg                                                                                                 >  2:127806/1‑45 (MQ=255)
                                                                                                                             gtgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcg                                                                                                 <  1:127806/45‑1 (MQ=255)
                                                                                                                             gtgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTgg                                >  1:703468/1‑110 (MQ=255)
                                                                                                                             gtgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTgg                                <  2:703468/110‑1 (MQ=255)
                                                                                                                              tgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATAc  >  2:42444/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                      
GCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATAC  >  NZ_CP009273/928070‑928334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑