Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	960,653	T→C	M293T (ATG→ACG)	ycaI →	ComEC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	960,653	0	T	C	100.0%	119.1 / NA	34	M293T (ATG→ACG)	ycaI	ComEC family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)

CCTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAATGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTC  >  NZ_CP009273/960526‑960777                                                                                                                                |                                                                                                                             ccTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCttaagtt                                                                                                                     >  2:763132/1‑137 (MQ=255)         cTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCAGGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGa                                                                                                           <  1:181362/139‑1 (MQ=255)                cACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtgg                                                                                                     <  1:290612/138‑1 (MQ=255)                cACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtgg                                                                                                     <  2:815005/138‑1 (MQ=255)                 aCTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTggg                                                                                                              <  1:144033/128‑1 (MQ=255)                 aCTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTggg                                                                                                              >  2:144033/1‑128 (MQ=255)                 aCTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                   >  1:322997/1‑139 (MQ=255)                 aCTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                   <  2:691490/139‑1 (MQ=255)                 aCTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                   <  1:739758/139‑1 (MQ=255)                     gCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGc                                                                                               <  2:848504/139‑1 (MQ=255)                         aTACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTggg                                                                                           <  2:85015/139‑1 (MQ=255)                         aTACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTggg                                                                                           <  2:309484/139‑1 (MQ=255)                          tACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGa                                                                                          >  2:790193/1‑139 (MQ=255)                              aTTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTa                                                                                      <  2:710069/139‑1 (MQ=255)                               ttAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCt                                                                                                                           <  1:168339/101‑1 (MQ=255)                               ttAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCt                                                                                                                           >  2:168339/1‑101 (MQ=255)                               ttAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                   >  2:546824/1‑125 (MQ=255)                               ttAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                   <  1:546824/125‑1 (MQ=255)                                   ttGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGat                                                                                 <  2:52721/139‑1 (MQ=255)                                                        ctTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAAtttt                                                            >  2:219606/1‑139 (MQ=255)                                                               tGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGAt                                                      <  1:444543/138‑1 (MQ=255)                                                                  cTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGAt                                                  >  1:540565/1‑139 (MQ=255)                                                                  cTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGAt                                                  >  1:345107/1‑139 (MQ=255)                                                                     gTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCt                                               >  1:588896/1‑139 (MQ=255)                                                                          cGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGc                                          >  1:213499/1‑139 (MQ=255)                                                                                      ctcctGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCa                              <  2:213499/139‑1 (MQ=255)                                                                                          tGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGt                          >  2:586480/1‑139 (MQ=255)                                                                                                 cGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTggg                                                                                                              >  1:789926/1‑48 (MQ=255)                                                                                                 cGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTggg                                                                                                              <  2:789926/48‑1 (MQ=255)                                                                                                 cGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCa                              >  1:58249/1‑128 (MQ=255)                                                                                                 cGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCa                              <  2:58249/128‑1 (MQ=255)                                                                                                      cATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGctctct              >  1:679243/1‑139 (MQ=255)                                                                                                      cATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGctctct              >  1:698517/1‑139 (MQ=255)                                                                                                                  tGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTc  >  1:804923/1‑139 (MQ=255)                                                                                                                                |                                                                                                                             CCTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAATGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTC  >  NZ_CP009273/960526‑960777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑