| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,079,189 | T→C | S120P (TCC→CCC) | efeB → | iron uptake transporter deferrochelatase/peroxidase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,079,189 | 0 | T | C | 92.9% | 79.8 / 0.1 | 28 | S120P (TCC→CCC) | efeB | iron uptake transporter deferrochelatase/peroxidase subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (15/11); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GTGGCGTTTGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGACGCG > NZ_CP009273/1079054‑1079316 | gtggCGTTTGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCg > 1:269147/1‑139 (MQ=255) gTTTGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCAtt > 1:859984/1‑139 (MQ=255) tttGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTc > 2:234342/1‑139 (MQ=255) cGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTgcg > 2:152060/1‑139 (MQ=255) aaGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCg < 2:809711/138‑1 (MQ=255) aGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGAt < 2:518844/139‑1 (MQ=255) aTCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTgg > 2:166180/1‑115 (MQ=255) aTCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTgg < 1:166180/115‑1 (MQ=255) aTCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAAtct > 2:219682/1‑139 (MQ=255) aGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCAt < 2:452455/139‑1 (MQ=255) gttgttTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACg < 1:234342/139‑1 (MQ=255) ttgttTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGt > 1:286805/1‑139 (MQ=255) gttTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTa > 1:793152/1‑139 (MQ=255) aGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAATCGCCAAATCCGCGCCTGCCACCTCTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTCTCGGTGGGTCACTCAt < 2:706692/138‑1 (MQ=255) aGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCAtt > 1:52079/1‑139 (MQ=255) cTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTtgatg < 2:461134/138‑1 (MQ=255) cAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGcc > 2:519349/1‑139 (MQ=255) agcagcACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCca > 2:719875/1‑139 (MQ=255) caCCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGAt > 2:309751/1‑139 (MQ=255) caCCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGAt > 1:550604/1‑139 (MQ=255) gAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTc > 2:190321/1‑98 (MQ=255) gAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTc < 1:190321/98‑1 (MQ=255) gAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCa > 1:513491/1‑138 (MQ=255) aaaCGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCaaa < 2:286805/139‑1 (MQ=255) gCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGAGGCTAAATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAg > 2:345619/1‑139 (MQ=255) aaaTCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTg < 1:236809/139‑1 (MQ=255) tCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCag < 1:845572/139‑1 (MQ=255) caccacTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCCCTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGAcgcg < 1:152060/139‑1 (MQ=255) | GTGGCGTTTGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGACGCG > NZ_CP009273/1079054‑1079316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |