Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,124,635	T→C	R447R (CGT→CGC)	murJ →	murein biosynthesis integral membrane protein MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,124,635	0	T	C	100.0%	154.1 / NA	45	R447R (CGT→CGC)	murJ	murein biosynthesis integral membrane protein MurJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/23);  total (22/23)

TTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCC  >  NZ_CP009273/1124499‑1124764                                                                                                                                         |                                                                                                                                  ttGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCt                                                                                                                                 >  2:846473/1‑139 (MQ=255)    gTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggt                                                                                                                              >  2:242470/1‑139 (MQ=255)         ttGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggtggtgg                                                                                                                         <  1:2655/139‑1 (MQ=255)         ttGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggtggtgg                                                                                                                         <  1:579653/139‑1 (MQ=255)              aCATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTa                                                                                                                    <  2:100675/139‑1 (MQ=255)                                 tCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGc                                                                                                     >  1:852283/1‑135 (MQ=255)                                 tCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGc                                                                                                     <  2:852283/135‑1 (MQ=255)                                     ttGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCtttt                                                                                             <  1:525748/139‑1 (MQ=255)                                     ttGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCttt                                                                                              >  2:525748/1‑138 (MQ=255)                                         tCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAgg                                                                                          >  1:164859/1‑138 (MQ=255)                                         tCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGt                                                                                         <  1:466544/139‑1 (MQ=255)                                             gcggcgTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGt                                                                                     >  2:544127/1‑139 (MQ=255)                                              cggcgTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTCAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGtt                                                                                    <  2:761796/139‑1 (MQ=255)                                                  gtgtCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACa                                                                                 >  2:69219/1‑138 (MQ=255)                                                   tgtCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGCTGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTTGAGTGCTTTTAGGCATGTTACata                                                                               >  1:128382/1‑139 (MQ=255)                                                   tgtCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTTCata                                                                               >  2:356954/1‑139 (MQ=255)                                                   tgtCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACata                                                                               >  1:677163/1‑139 (MQ=255)                                                         aaTGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATg                                                                          <  1:846473/138‑1 (MQ=255)                                                             cTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCg                                                                       >  1:521212/1‑137 (MQ=255)                                                             cTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGa                                                                     <  1:453349/139‑1 (MQ=255)                                                              ttCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAg                                                                    >  1:98030/1‑139 (MQ=255)                                                               tCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg                                                                                                    <  1:283387/106‑1 (MQ=255)                                                               tCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg                                                                                                    >  2:283387/1‑106 (MQ=255)                                                                cGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTg                                                                  >  2:533730/1‑139 (MQ=255)                                                                 gctgctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTgg                                                                 <  2:169714/139‑1 (MQ=255)                                                                     ctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATTTCATGCCGGAGTGGTCAt                                                             >  2:88758/1‑139 (MQ=255)                                                                     ctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCAt                                                             >  1:1919/1‑139 (MQ=255)                                                                        tACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCAtt                                                            <  1:544127/137‑1 (MQ=255)                                                                        tACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCAtt                                                            <  1:489057/137‑1 (MQ=255)                                                                        tACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTg                                                           <  2:376070/138‑1 (MQ=255)                                                                             gCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTAcc                                                     <  2:174904/139‑1 (MQ=255)                                                                               aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACata                                                                               <  2:649162/111‑1 (MQ=255)                                                                               aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACata                                                                               >  1:649162/1‑111 (MQ=255)                                                                                   gCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGcc                                                <  2:98030/138‑1 (MQ=255)                                                                                   gCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGcc                                                <  2:1919/138‑1 (MQ=255)                                                                                            gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACata                                                                               >  2:690632/1‑98 (MQ=255)                                                                                            gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACata                                                                               <  1:690632/98‑1 (MQ=255)                                                                                                     tACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg                                                                                                    <  2:471160/68‑1 (MQ=255)                                                                                                     tACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg                                                                                                    >  1:471160/1‑68 (MQ=255)                                                                                                          cGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGc                        >  1:697083/1‑139 (MQ=255)                                                                                                            cAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCgg                      >  2:707294/1‑139 (MQ=255)                                                                                                                 cGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTg                 <  1:707294/139‑1 (MQ=255)                                                                                                                   gCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCt               >  1:214747/1‑139 (MQ=255)                                                                                                                       gATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGc            <  1:242470/138‑1 (MQ=255)                                                                                                                                 cTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGcc  <  2:164859/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  TTGGTCCGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCC  >  NZ_CP009273/1124499‑1124764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑