Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,161,212	T→C	intergenic (+71/‑329)	ycfP → / → ndh	alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,161,212	0	T	C	100.0%	157.8 / NA	46	intergenic (+71/‑329)	ycfP/ndh	alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/29);  total (17/29)

CACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTC  >  NZ_CP009273/1161079‑1161340                                                                                                                                      |                                                                                                                                 cacaAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCAcg                                                                                                                             <  1:514997/139‑1 (MQ=255)           aGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGa                                                                                                                   <  1:101497/139‑1 (MQ=255)           aGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCGCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGa                                                                                                                   <  1:137791/139‑1 (MQ=255)                               cAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCt                                                                                                >  2:839086/1‑138 (MQ=255)                                  cgcATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATg                                                                                            <  1:809698/139‑1 (MQ=255)                                  cgcATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATg                                                                                            <  1:232839/139‑1 (MQ=255)                                    cATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCAcg                                                                                                                             >  1:593410/1‑104 (MQ=255)                                    cATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCAcg                                                                                                                             <  2:593410/104‑1 (MQ=255)                                      ttAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGct                                                                                        <  2:27222/139‑1 (MQ=255)                                      ttAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGct                                                                                        <  1:11967/139‑1 (MQ=255)                                                        tCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCg                                                                       >  1:243265/1‑138 (MQ=255)                                                         cGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGaa                                                                     >  2:108231/1‑139 (MQ=255)                                                             tAACTGCCCTCGCCGCATCAGGTACCCTTACCGGTCCCTGATTCGCCCCGAATTCTGTGGGTCGGCTAAAACGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAAAGcc                                                                 <  2:255814/139‑1 (MQ=255)                                                             tAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc                                                                 <  2:243265/139‑1 (MQ=255)                                                             tAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc                                                                 <  1:529948/139‑1 (MQ=255)                                                               aaTGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGccc                                                                <  2:614429/138‑1 (MQ=255)                                                               aaTGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCt                                                               >  2:37398/1‑139 (MQ=255)                                                                    ccTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATa                                                          <  1:349195/139‑1 (MQ=255)                                                                     cTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATac                                                         <  1:477323/139‑1 (MQ=255)                                                                     cTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATac                                                         <  1:377562/139‑1 (MQ=255)                                                                      tcgtcgCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATaca                                                        >  2:819544/1‑139 (MQ=255)                                                                           gCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACAcccc                                                    <  1:735520/138‑1 (MQ=255)                                                                           gCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                   <  1:688130/139‑1 (MQ=255)                                                                             aTCAGGTAACCTTGCCGTTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCa                                                 <  2:316157/139‑1 (MQ=255)                                                                             aTCAGGTAACCTTGCCGGTTCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCa                                                 <  2:438908/139‑1 (MQ=255)                                                                             aTCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCa                                                 <  1:108231/139‑1 (MQ=255)                                                                                aGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCAct                                               <  2:617228/138‑1 (MQ=255)                                                                                       cTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTAt                                                                                  <  2:486770/96‑1 (MQ=255)                                                                                       cTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTAt                                                                                  >  1:486770/1‑96 (MQ=255)                                                                                       cTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCa                                       >  2:369537/1‑139 (MQ=255)                                                                                       cTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCa                                       >  2:566052/1‑139 (MQ=255)                                                                                         tGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                   <  1:862706/125‑1 (MQ=255)                                                                                         tGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                   >  2:862706/1‑125 (MQ=255)                                                                                          gCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActc                                    >  2:72037/1‑139 (MQ=255)                                                                                           ccGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAAc                                                                    <  1:677619/106‑1 (MQ=255)                                                                                           ccGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAAc                                                                    >  2:677619/1‑106 (MQ=255)                                                                                           ccGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct                                   >  2:508577/1‑139 (MQ=255)                                                                                            cGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc                                                                 <  1:63667/108‑1 (MQ=255)                                                                                            cGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc                                                                 >  2:63667/1‑108 (MQ=255)                                                                                                        cgcTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTc                      <  1:294252/139‑1 (MQ=255)                                                                                                          cTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCAct                                               <  1:594965/112‑1 (MQ=255)                                                                                                          cTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCAct                                               >  2:594965/1‑112 (MQ=255)                                                                                                                     gtgGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaacaa         >  2:636033/1‑139 (MQ=255)                                                                                                                            ggATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAActc  <  1:839086/139‑1 (MQ=255)                                                                                                                              aTAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                   <  1:88373/88‑1 (MQ=255)                                                                                                                              aTAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                   >  2:88373/1‑88 (MQ=255)                                                                                                                                      |                                                                                                                                 CACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTC  >  NZ_CP009273/1161079‑1161340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑