| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 120,914 | A→C | T471P (ACC→CCC) | aceE → | pyruvate dehydrogenase (acetyl‑transferring), homodimeric type |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 120,914 | 0 | A | C | 75.4% | 126.1 / 42.1 | 61 | T471P (ACC→CCC) | aceE | pyruvate dehydrogenase (acetyl‑transferring), homodimeric type |
| Reads supporting (aligned to +/- strand): ref base A (8/7); new base C (22/24); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
TGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGA > NZ_CP009273/120789‑121044 | tgtCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCtt > 1:735889/1‑137 (MQ=255) tGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTg > 2:765345/1‑139 (MQ=255) aTGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGcc > 1:241640/1‑139 (MQ=255) aTGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGcc > 2:766354/1‑139 (MQ=255) tGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGcc > 1:584358/1‑138 (MQ=255) atatCGAAAAACTGCCGTACATCACCTTCCCGGCAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGACAAGCCGTCAGCCGCACTTCACCGAGAAGCTTGAGCTGCCGAGCCt > 2:40238/1‑139 (MQ=255) atatCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCt > 1:202809/1‑139 (MQ=255) atatCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCt > 2:42751/1‑139 (MQ=255) tatCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTg < 2:735889/139‑1 (MQ=255) cgtccgtCACCTTCCCGGAAGGTTCTGAAGAGCATCCCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgc < 2:207038/133‑1 (MQ=255) cGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgc < 2:378150/139‑1 (MQ=255) gTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTACCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgcg < 1:2946/139‑1 (MQ=255) gTACAGCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTGCGgcgcg < 2:26371/139‑1 (MQ=255) cACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTg > 2:351158/1‑139 (MQ=255) aCCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTgg < 1:766354/139‑1 (MQ=255) ccTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCt < 1:841595/133‑1 (MQ=255) ccTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCt > 2:841595/1‑133 (MQ=255) tCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgcg > 1:418160/1‑128 (MQ=255) tCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgcg < 2:418160/128‑1 (MQ=255) cccGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagag > 1:493400/1‑139 (MQ=255) ccGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagagc < 1:510212/139‑1 (MQ=255) ccGGAAGGTTCTGAAGAGCATACCTACCGTCACGCTCAGCGTCAGAAACTGCACGGTTATCCGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagagc < 2:219508/139‑1 (MQ=255) tCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAgagcagagca > 2:733540/1‑136 (MQ=255) tCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagagc > 2:452650/1‑130 (MQ=255) tCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagagc < 1:452650/130‑1 (MQ=255) aaGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGAt < 1:493257/139‑1 (MQ=255) aaGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAagag < 2:584358/137‑1 (MQ=255) aaGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAagag < 1:493246/137‑1 (MQ=255) aaGAGCATACCTATCTGCACGCGCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAagag < 2:22752/137‑1 (MQ=255) cATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgc < 2:531498/107‑1 (MQ=255) cATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgc > 1:531498/1‑107 (MQ=255) cATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGt > 1:82890/1‑112 (MQ=255) cATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGt < 2:82890/112‑1 (MQ=255) cATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTa > 2:344129/1‑139 (MQ=255) cATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTa > 2:460631/1‑139 (MQ=255) cATACCTATCTGCACGCCCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTa > 2:821088/1‑139 (MQ=255) tACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTAc > 2:324659/1‑138 (MQ=255) tCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAagag < 1:91107/124‑1 (MQ=255) tCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAagag > 2:91107/1‑124 (MQ=255) tCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATc > 2:10016/1‑139 (MQ=255) tCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATc > 2:530557/1‑139 (MQ=255) tCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATc > 2:358600/1‑139 (MQ=255) tGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGc > 1:117486/1‑139 (MQ=255) aGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAaga > 1:133306/1‑112 (MQ=255) aGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAaga < 2:133306/112‑1 (MQ=255) aGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGctct < 1:593892/139‑1 (MQ=255) cTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACg < 1:460631/139‑1 (MQ=255) cACGGTTATCTGCCAAGCCGTCAGCCGAACTACCCCGAGAAGCTTGAGCTGCCGAGCCTg < 2:699930/60‑1 (MQ=255) cACGGTTATCTGCCAAGCCGTCAGCCGAACTACCCCGAGAAGCTTGAGCTGCCGAGCCTg > 1:699930/1‑60 (MQ=255) gTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCt < 2:148599/139‑1 (MQ=255) ccAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTgaagaa < 1:765345/137‑1 (MQ=255) aaGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAg < 1:324659/139‑1 (MQ=255) aGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAg < 1:196530/138‑1 (MQ=255) aGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAg > 2:196530/1‑138 (MQ=255) aGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGt < 2:117486/139‑1 (MQ=255) aGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGt < 1:54972/139‑1 (MQ=255) aGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGt > 2:614786/1‑139 (MQ=255) gCCGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCaaa < 2:371155/139‑1 (MQ=255) cGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGa < 1:344129/139‑1 (MQ=255) cGAACTTCCCCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGa < 1:153450/139‑1 (MQ=255) aaCTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCa > 2:414989/1‑133 (MQ=255) | TGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCAAAGAGATCTCTACCACTATCGCTTTCGTTCGTGCTCTGAACGTGATGCTGAAGAACAAGTCGATCAAAGA > NZ_CP009273/120789‑121044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |