Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,220,495	T→A	D108V (GAT→GTT)	minD ←	septum site‑determining protein MinD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,220,495	0	T	A	100.0%	112.2 / NA	28	D108V (GAT→GTT)	minD	septum site‑determining protein MinD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/15);  total (13/15)

AGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCC  >  NZ_CP009273/1220373‑1220622                                                                                                                           |                                                                                                                                 aggagACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGc                                                                                                                  >  2:130863/1‑139 (MQ=255)   gagaCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGa                                                                                                                >  2:203479/1‑139 (MQ=255)   gagaCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGa                                                                                                                >  1:658009/1‑139 (MQ=255)      aCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAccc                                                                                                             <  1:472674/139‑1 (MQ=255)           cGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCa                                                                                                        >  1:702213/1‑139 (MQ=255)                 ggtggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGt                                                                                                  <  1:130863/138‑1 (MQ=255)                   tggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAg                                                                                               <  2:231952/139‑1 (MQ=255)                     gtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAggg                                                                                             <  1:353543/139‑1 (MQ=255)                            tGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCtt                                                                                       >  1:113358/1‑138 (MQ=255)                                    tCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCgtg                                                                              <  1:511824/139‑1 (MQ=255)                                                  tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAc                                                                                                               <  1:686465/93‑1 (MQ=255)                                                  tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAc                                                                                                               >  2:686465/1‑93 (MQ=255)                                                  tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACCGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCgg                                                                 >  1:792338/1‑139 (MQ=255)                                                  tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATcg                                                                                  >  2:638296/1‑121 (MQ=255)                                                  tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATcg                                                                                  <  1:638296/121‑1 (MQ=255)                                                    ccATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACCGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCa                                                               <  2:792338/139‑1 (MQ=255)                                                    ccATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCa                                                               <  2:113358/138‑1 (MQ=255)                                                               ccGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAt                                                   <  2:573745/139‑1 (MQ=255)                                                                           ccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCt                                       <  2:112236/139‑1 (MQ=255)                                                                               ccGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATc                                   >  2:785492/1‑139 (MQ=255)                                                                                 ggggAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCtt                                 <  1:72066/139‑1 (MQ=255)                                                                                              cGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCt                     >  2:593193/1‑138 (MQ=255)                                                                                                         aaaTCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTg                                                                                                 >  1:742422/1‑51 (MQ=255)                                                                                                         aaaTCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTg                                                                                                 <  2:742422/51‑1 (MQ=255)                                                                                                           aTCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCa       <  2:30033/139‑1 (MQ=255)                                                                                                               aTCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGc   >  2:510801/1‑139 (MQ=255)                                                                                                               aTCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGc   >  1:239785/1‑139 (MQ=255)                                                                                                                tCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGcc  <  1:130518/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                 AGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCA‑CGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCC  >  NZ_CP009273/1220373‑1220622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑