Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,223,250 T→C V27V (GTT→GTC)  BW25113_RS06140 → YcgL domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,223,2500TC93.9% 108.7 / ‑0.4 33V27V (GTT→GTC) BW25113_RS06140YcgL domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (18/13);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAG  >  NZ_CP009273/1223116‑1223385
                                                                                                                                      |                                                                                                                                       
gggTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTg                                                                                                                                     <  2:48972/139‑1 (MQ=255)
            tGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACtgatg                                                                                                                          >  1:340023/1‑138 (MQ=255)
            tGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACtgatg                                                                                                                          >  1:35917/1‑138 (MQ=255)
              ttAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGAGTTTTCGCGTGTCCCTGAGGAACTGATGaa                                                                                                                        <  2:595609/138‑1 (MQ=255)
                     aTGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTg                                                                                                                >  2:66219/1‑139 (MQ=255)
                      tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTgg                                                                                                               >  2:143595/1‑139 (MQ=255)
                       gCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                              >  1:714669/1‑139 (MQ=255)
                        ccAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                              <  2:210625/138‑1 (MQ=255)
                           aaaCCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGc                                                                                                          >  2:860484/1‑139 (MQ=255)
                            aaCCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGcc                                                                                                         >  1:211996/1‑139 (MQ=255)
                                 ggCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGt                                                                                                    >  1:372847/1‑139 (MQ=255)
                                                  aaGTCTGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCg                                                                                   <  1:489611/139‑1 (MQ=255)
                                                              tgtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCg                                                                        >  2:553504/1‑138 (MQ=255)
                                                                tgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGga                                                                      <  1:814356/138‑1 (MQ=255)
                                                                 gATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCgg                                                                       >  2:482858/1‑136 (MQ=255)
                                                                     tATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGaaa                                                                 <  1:860484/138‑1 (MQ=255)
                                                                     tATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGaaa                                                                 <  2:799562/138‑1 (MQ=255)
                                                                        cGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTgg                                                             >  2:68566/1‑139 (MQ=255)
                                                                                  aGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGa                                                   <  1:553504/139‑1 (MQ=255)
                                                                                         ccAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGaa                                            <  2:211996/139‑1 (MQ=255)
                                                                                             aCCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAgg                                        >  1:145085/1‑139 (MQ=255)
                                                                                             aCCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACCGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAgg                                        >  2:252258/1‑139 (MQ=255)
                                                                                                  tttATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTaaa                                   <  1:34869/139‑1 (MQ=255)
                                                                                                       atGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAgg                               >  2:168484/1‑138 (MQ=255)
                                                                                                        tGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCa                             >  2:318358/1‑139 (MQ=255)
                                                                                                         gTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCAt                            >  2:498619/1‑139 (MQ=255)
                                                                                                           gaaaaaaaaaGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCAtt                           >  2:125538/3‑138 (MQ=255)
                                                                                                                 aaaaGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAg                    <  1:482858/139‑1 (MQ=255)
                                                                                                                  aaaGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGc                   <  2:35917/139‑1 (MQ=255)
                                                                                                                   aaGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGc                   >  1:60729/1‑138 (MQ=255)
                                                                                                                      aCGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAgg               <  2:714669/139‑1 (MQ=255)
                                                                                                                        gATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGtt             <  1:76472/139‑1 (MQ=255)
                                                                                                                                   tgtCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAg  >  1:795467/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTTACTATTTGCAG  >  NZ_CP009273/1223116‑1223385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑