Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,235,908	T→G	A540A (GCA→GCC)	cvrA ←	potassium/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,235,908	0	T	G	100.0%	125.0 / NA	37	A540A (GCA→GCC)	cvrA	potassium/proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/19);  total (18/19)

TAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCAC  >  NZ_CP009273/1235774‑1236038                                                                                                                                       |                                                                                                                                   tAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCcg                                                                                                                                <  1:149311/139‑1 (MQ=255)        ttCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTaa                                                                                                                         >  1:265523/1‑139 (MQ=255)          cATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTaac                                                                                                                        <  1:24786/138‑1 (MQ=255)                    aaGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGa                                                                                                              <  2:112782/138‑1 (MQ=255)                         tCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCcg                                                                                                                                >  1:677879/1‑115 (MQ=255)                         tCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCcg                                                                                                                                <  2:677879/115‑1 (MQ=255)                                            cccGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGa                                                                                                              <  2:749936/114‑1 (MQ=255)                                            cccGTACGCCAATCTTCAACACTTCATTATCTACTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGa                                                                                                              >  1:749936/1‑114 (MQ=255)                                                tACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCa                                                                                 >  2:611480/1‑139 (MQ=255)                                                tACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCa                                                                                 <  1:357176/139‑1 (MQ=255)                                                       aTCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATAtt                                                                          >  1:384594/1‑139 (MQ=255)                                                               cacTTCATTATCTTCTTTCTCGGCCACCGGCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCTCCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCg                                                                  <  1:649336/139‑1 (MQ=255)                                                                     aTTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTc                                                             <  2:451081/138‑1 (MQ=255)                                                                                   cGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCcg                                                                                                                                <  2:61410/57‑1 (MQ=255)                                                                                   cGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCcg                                                                                                                                >  1:61410/1‑57 (MQ=255)                                                                                    ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg                                                         <  1:482551/127‑1 (MQ=255)                                                                                    ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg                                                         >  2:482551/1‑127 (MQ=255)                                                                                    ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATc                                                <  1:611480/136‑1 (MQ=255)                                                                                      ccACCGTCCAGATCATCCCGGCAAACTCTACCTTGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcgc                                           <  2:429146/139‑1 (MQ=255)                                                                                      ccACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcgc                                           <  1:627192/139‑1 (MQ=255)                                                                                      ccACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcgc                                           <  2:265523/139‑1 (MQ=255)                                                                                                    atcCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATAtt                             >  1:527056/1‑139 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCcg                                                                                                                                >  1:17337/1‑38 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCcg                                                                                                                                <  2:17337/38‑1 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa                                                           <  2:508988/107‑1 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa                                                           >  1:508988/1‑107 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat                                                 >  2:522026/1‑117 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat                                                 <  1:522026/117‑1 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg                           >  1:78134/1‑139 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg                           >  2:616794/1‑139 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg                           >  2:708065/1‑139 (MQ=255)                                                                                                      cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg                           >  1:447771/1‑139 (MQ=255)                                                                                                         ggCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGgcg                        <  2:384594/139‑1 (MQ=255)                                                                                                                  tACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCtt                 <  1:616794/137‑1 (MQ=255)                                                                                                                       ggTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAAt          >  2:147862/1‑139 (MQ=255)                                                                                                                          cACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATg         >  2:632632/1‑137 (MQ=255)                                                                                                                               aCAACCGGGGCCGCGCCTAACAACTGCTGGACACTTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGACTGAAGTCAc  >  2:686196/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                   TAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCAC  >  NZ_CP009273/1235774‑1236038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑